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Using cDNA SCoT method to identify differentially expressed genes in date palm (Phoenix dactylifera L.)

Published online by Cambridge University Press:  03 April 2023

Soumaya Rhouma Open the ORCID record for Soumaya Rhouma [Opens in a new window] ,
Fatma Saad Open the ORCID record for Fatma Saad [Opens in a new window] ,
Ahlem Ben Saad Open the ORCID record for Ahlem Ben Saad [Opens in a new window] ,
Maha Moussa Open the ORCID record for Maha Moussa [Opens in a new window]  and
Khaled Chatti Open the ORCID record for Khaled Chatti [Opens in a new window]
Soumaya Rhouma*
Affiliation:
Laboratory of Genetics, Biodiversity and Bioresources Valorisation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Fatma Saad
Affiliation:
Laboratory of Genetics, Biodiversity and Bioresources Valorisation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Ahlem Ben Saad
Affiliation:
Laboratory of Genetics, Biodiversity and Bioresources Valorisation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Maha Moussa
Affiliation:
Laboratory of Genetics, Biodiversity and Bioresources Valorisation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Khaled Chatti
Affiliation:
Laboratory of Genetics, Biodiversity and Bioresources Valorisation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
*
Author for correspondence: Soumaya Rhouma, E-mail: rhoumasoumaya@yahoo.fr
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Abstract

Date palm is a relatively salt- and drought-tolerant plant and more recent efforts have been focused on recognizing genes and pathways that confer stress tolerance in this species. The cDNA start codon targeted marker (cDNA–SCoT) technique is a novel, simple, fast and effective method for differential gene expression investigation. In the present study, this technique was exploited to identify differentially expressed genes during several stress treatments in date palm. A total of 12 SCoT primers combined with oligo-dT primers amplified differentially expressed fragments among the stress treatments and control samples. Differentially expressed fragments were highly homologous to known genes or encoded unclassified proteins with unknown functions. The expression patterns of the genes that had direct or indirect relationships with salinity and drought stress response were identified and their possible roles were discussed. This study suggests that the cDNA-SCoT differential display method is a useful tool to serve as an initial step for characterizing transcriptional changes induced by abiotic stresses and provide gene information for further study and application in genetic improvement and breeding in date palm.

Keywords

Abiotic stresscDNA-SCoTPhoenix dactylifera Ltranscript-derived fragmentstranscriptome analysis
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 4 , August 2022 , pp. 297 - 303
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of NIAB

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