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Study of symptoms and gene expression in four Pinus species after pinewood nematode infection

Published online by Cambridge University Press:  15 June 2011

Albina R. Franco ,
Carla Santos ,
Mariana Roriz ,
Rui Rodrigues ,
Marta R. M. Lima  and
Marta W. Vasconcelos
Albina R. Franco
Affiliation:
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072Porto, Portugal
Carla Santos
Affiliation:
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072Porto, Portugal
Mariana Roriz
Affiliation:
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072Porto, Portugal
Rui Rodrigues
Affiliation:
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072Porto, Portugal
Marta R. M. Lima
Affiliation:
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072Porto, Portugal
Marta W. Vasconcelos*
Affiliation:
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072Porto, Portugal
*
*Corresponding author. E-mail: mwvasconcelos@esb.ucp.pt
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Abstract

Pine wilt disease, caused by the pinewood nematode Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle, is originating severe infections in pine trees. The disease is detected when external symptoms appear (e.g. needle chlorosis), but trees could remain asymptomatic for long periods and serve as a long-term host. The primary goal of this study was to assess the effect of inoculation with an avirulent isolate of B. xylophilus (C14-5) on different Pinus spp. seedlings (P. sylvestris, P. nigra, P. pinea and P. pinaster). At the same time, seedlings were also inoculated with a virulent strain, HF, in order to compare the phenotypic and genomic results of the two types of inoculations. The effect of inoculation was determined in terms of expression of various Pinus genes potentially involved in the response to the disease.The results suggest that P. pinea and P. nigra are more resistant to infection by the nematode than P. sylvestris and P. pinaster. The phenotypic and genetic differences were more marked among P. pinea and P. pinaster.

Keywords

Bursaphelenchus xylophilusgenetic expressionPinus spp
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 272 - 275
Copyright
Copyright © NIAB 2011

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References

Asai, E and Futai, K (2002) Promotion of the population growth of pinewood nematode in 4-month-old. Journal of Forestry Research 7: 113–116.CrossRefGoogle Scholar
OEPP/EPPO (2001) Bursaphelenchus xylophilus. PM 7/4 (1) OEPP/EPPO Bulletin 31: 61–69.Google Scholar
Fogain, R and Gowen, SR (1996) Investigations on possible mechanisms of resistance to nematodes in Musa. Euphytica 92: 375–381.CrossRefGoogle Scholar
Fukuda, K (1997) Physiological process of the symptom development and resistance mechanism in pine wilt disease. Journal of Forest Research 2: 171–181.CrossRefGoogle Scholar
Jones, J, Moens, M, Mota, M, Li, H and Kikuchi, I (2008) Bursaphelenchus xylophilus: opportunities in comparative genomics and molecular host–parasite interactions. Molecular Plant Pathology 9: 357–368.CrossRefGoogle ScholarPubMed
Kawaguchi, E (2006) Relationship between the anatomical characteristics of cortical resin canals and migration of Bursaphelenchus xylophilus in stem cuttings of Pinus thunbergii seedlings. Journal of the Japanese Forest Society 88: 240–244.CrossRefGoogle Scholar
Kosaka, H, Aikawa, T, Ogura, N, Tabata, K and Kiyohara, T (2001) Pine wilt disease caused by the pine wood nematode: the induced resistance of pine trees by the avirulent isolates of nematode. European Journal of Plant Pathology 107: 667–675.CrossRefGoogle Scholar
Kuroda, K (2004) Inhibiting factors of symptom development in several Japanese red pine (Pinus densiflora) families selected as resistant to one wilt. Journal of Forestry Research 9: 217–224.CrossRefGoogle Scholar
Le Provost, G, Herrera, R, Paiva, J, Chaumeil, P, Salin, FF and Plomion, C (2007) A micromethod for high throughput RNA extraction in forest trees. Biological Research 40: 291–297.CrossRefGoogle ScholarPubMed
Meins, F and Ahl, F (1989) Induction of chitinase and [beta]-1,3-glucanase in tobacoo plants infected with Pseudomonas tabaci and Phytophythora parasitica var. nicotianae. Plant Physiology 61: 155–161.Google Scholar
Miller, B, Madilao, L, Ralph, S and Bohlmann, J (2005) Insect-induced conifer defense, White pine weevil and methyl jasmonate induce traumatic resinosis, de novo formed volatile emissions, and accumulation of terpenoid synthase and putative octadecanoid pathway transcripts in Sitka spruce. Plant Physiology 137: 369–382.CrossRefGoogle ScholarPubMed
Mir, G, Demenech, G, Huguet, G, Guo, W, Goldsbrough, P, Atrian, S, Molinas, M (2004) A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress. Journal of Experimental Botany 55: 2483–2493.CrossRefGoogle ScholarPubMed
Mota, MM, Braasch, H, Bravo, MA, Penas, AC, Burgermeister, W, Metge, K and Sousa, E (1999) First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1: 727–734.CrossRefGoogle Scholar
Osmond, R, Hrmova, J, Fontaine, F, Imberty, A and Fincher, G (2001) Binding interactions between barley thaumatin-like proteins and (1,3)-β-d-glucans. European Journal of Biochemistry 268: 4190–4199.CrossRefGoogle Scholar
Shin, H, Lee, H, Woo, K-S, Noh, E-W, Koo, Y-B and Lee, K-J (2009) Identification of genes upregualted by pinewood nematode inoculation in Japanese red pine. Tree Physiology 29: 411–421.CrossRefGoogle Scholar
Takehushi, Y, Kanzaki, N and Futai, K (2006) How different is induced resistance against the pine wood nematode, Bursaphelenchus xylophilus, by two avirulent microbes? Nematology 8: 435–442.Google Scholar