Hostname: page-component-8448b6f56d-m8qmq Total loading time: 0 Render date: 2024-04-24T00:19:58.025Z Has data issue: false hasContentIssue false
  • English
  • Français

New pepper accessions proved to be suitable as a genetic resource for use in breeding nematode-resistant rootstocks

Published online by Cambridge University Press:  03 February 2015

Fulgencio Sánchez-Solana ,
Caridad Ros ,
María del Mar Guerrero ,
Carmen María Lacasa ,
Elena Sánchez-López  and
Alfredo Lacasa
Fulgencio Sánchez-Solana*
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, IMIDA, C/Mayor s/n, 30150La Alberca (Murcia), Spain
Caridad Ros
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, IMIDA, C/Mayor s/n, 30150La Alberca (Murcia), Spain
María del Mar Guerrero
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, IMIDA, C/Mayor s/n, 30150La Alberca (Murcia), Spain
Carmen María Lacasa
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, IMIDA, C/Mayor s/n, 30150La Alberca (Murcia), Spain
Elena Sánchez-López
Affiliation:
Departamento de Hortofruticultura, IMIDA, C/Mayor s/n, 30150La Alberca (Murcia), Spain
Alfredo Lacasa
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, IMIDA, C/Mayor s/n, 30150La Alberca (Murcia), Spain
*
*Corresponding author. E-mail: fsanchezsolana@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Root-knot nematodes (RKNs), Meloidogyne spp., are considered, worldwide, as one of the main pathogens of solanaceous crops, including pepper (Capsicum spp.). Restrictions on the use of standard nematicides have motivated the development and use of resistant cultivars and rootstocks. Three genes in pepper, called Me1, Me3 and N, confer resistance to the three main RKN species (Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria). However, their effectiveness seems to be limited because nematode populations that have overcome the resistance have been found, leading to a search for new sources of resistance and strategies to preserve their effectiveness. In two greenhouses and over a 7-month growing period, we evaluated the resistance to M. incognita and the agronomic behaviour as rootstocks of nine pepper (Capsicum annuum) accessions – HDA330 (Me1 carrier), Serrano Criollo de Morelos, (Me3 carrier), Yolo Wonder (partially resistant) and another six accessions of unknown resistance originating from cultivars well adapted to the local growing conditions. The resistance conferred by the Me1 gene was more robust than that conferred by Me3. Resistance to M. incognita was found in four new accessions: P13, CTL, CT5, and P14. In P13, the level of resistance was similar to that of HDA330. The resistant accessions showed better agronomic behaviour than the susceptible accessions, which was most noticeable towards the final of the growing period. Some accessions constitute a potential resource for use in the genetic breeding of RKN-resistant rootstocks.

Keywords

Capsicum annuumgenetic resistancegraftinggreenhouseMeloidogyne incognita
Type
Research Article
Information
Plant Genetic Resources , Volume 14 , Issue 1 , March 2016 , pp. 28 - 34
Copyright
Copyright © NIAB 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abu Irmaileh, BE, Mansour, AN, Al Banna, LS and Badwan, HO (2013) Comparative tolerances of two Cucumis species to salinity, Rhizoctonia solani and Meloidogyne incognita . Plant Genetic Resources: Characterization and Utilization 12: 178–184.Google Scholar
Barbary, A, Palloix, A, Fazari, A, Marteu, N, Castagnone-Sereno, P and Djian-Caporalino, C (2014) The plant genetic background affects the efficiency of the pepper major nematode resistance genes Me1 and Me3 . Theoretical and Applied Genetics 127: 499–507.CrossRefGoogle ScholarPubMed
Bridge, JS and Page, LJ (1980) Estimation of root-knot nematodes infestation levels on roots using a rating chart. Tropical Pest Management 26: 296–298.Google Scholar
Castagnone-Sereno, P, Bongiovanni, M, Palloix, A and Dalmasso, A (1996) Selection for Meloidogyne incognita virulence against resistance genes from tomato and pepper and specificity of the virulence/resistance determinants. European Journal of Plant Pathology 102: 585–590.CrossRefGoogle Scholar
Colla, P, Gillardi, G and Gullino, ML (2012) A review and critical analysis of the European situation of soilborne disease management in the vegetable sector. Phytoparasitica 40: 515–523.Google Scholar
Di Vito, M, Greco, N and Carrela, A (1985) Population densities of Meloidogyne incognita and yield of Capsicum annuum . Journal of Nematology 17: 45–49.Google Scholar
Djian-Caporalino, C, Pijarowski, L, Januel, A, Lefebvre, V, Daubeze, A, Palloix, A, Dalmasso, A and Abad, P (1999) Spectrum of resistance to root-knot nematodes and inheritance of heat-stable resistance in pepper (Capsicum annuum L.). Theoretical and Applied Genetics 99: 496–502.Google Scholar
Djian-Caporalino, C, Pijarowski, L, Fazari, A, Samson, M, Gaveau, L, O'Byrne, C, Lefebvre, V, Caranta, C, Palloix, A and Abad, P (2001) High-resolution genetic mapping of the pepper (Capsicum annuum L.) resistance loci Me 3 and Me 4 conferring heat-stable resistance to root-knot nematodes (Meloidogyne spp.). Theoretical and Applied Genetics 103: 592–600.Google Scholar
Djian-Caporalino, C, Molinari, S, Palloix, A, Ciancio, A, Fazari, A, Marteu, N, Ris, N and Castagnone-Sereno, P (2011) The reproductive potential of the root-knot nematode Meloidogyne incognita is affected by selection for virulence against major resistance genes from tomato and pepper. European Journal of Plant Pathology 131: 431–440.CrossRefGoogle Scholar
Djian-Caporalino, C, Palloix, A, Fazari, A, Marteu, N, Barbary, A, Abad, P, Sage-Palloix, AM, Mateille, T, Risso, S, Lanza, R, Taussig, C and Castagnone-Sereno, P (2014) Pyramiding, alternating or mixing: comparative performances of deployment strategies of nematode resistance genes to promote plant resistance efficiency and durability. BioMed Central Plant Biology 14: 53.CrossRefGoogle ScholarPubMed
Ehwaeti, ME, Phillips, MS and Trudgill, DL (1998) Dynamics of damage to tomato by Meloidogyne incognita . Fundamental and Applied Nematology 21: 627–635.Google Scholar
Fazari, A, Palloix, A, Wang, LH, Hua, MY, Sage-Palloix, AM, Zhang, BX and Djian-Caporalino, C (2012) The root-knot nematode resistance N-gene co-localizes in the Me-genes cluster on the pepper (Capsicum annuum L.) P9 chromosome. Plant Breeding 131: 665–673.Google Scholar
Guerrero-Díaz, MM, Lacasa-Marínez, CM, Hernández-Piñera, A, Martínez-Alarcón, V and Lacasa-Plasencia, A (2013) Evaluation of repeated biodisinfestation using Brassica carinata pellets to control Meloidogyne incognita in protected pepper crops. Spanish Journal of Agricultural Research 11: 485–493.CrossRefGoogle Scholar
Hare, WW (1956) Resistance in pepper to Meloidogyne incognita acrita . Phytopathology 46: 98–104.Google Scholar
Hendy, H, Pochard, E and Damalso, A (1985) Transmission héréditaire de la résistance aux nématodes Meloidogyne Chitwood (Tylenchida) portée par 2 lignées de Capsicum anuum L.: étude de descendances homozygotes issues d'androgenése. Agronomie 5: 93–100.Google Scholar
Jang, Y, Moon, JH, Lee, JW, Lee, SG, Kim, SY and Chun, C (2013) Effects of different rootstocks on fruit quality of grafted pepper (Capsicum annuum L.). Korean Journal of Horticultural Science and Technology 31: 687–699.Google Scholar
Kamran, M, Anwar, SA, Javed, N, Khan, SA, Abbas, H, Iqbal, MA and Zohaib, A (2013) The influence of Meloidogyne incognita density on susceptible tomato. Pakistan Journal of Zoology 45: 727–732.Google Scholar
Khan, MW and Haider, SH (1991) Comparative damage potential and reproduction efficiency of Meloidogyne javanica and races of Meloidogyne incognita on tomato and eggplant. Nematologica 37: 293–303.Google Scholar
Leal-Fernández, C, Godoy-Hernández, H, Núñez-Colín, CA, Anaya-López, JL, Villalobos-Reyes, S and Castellanos, JZ (2013) Morphological response and fruit yield of sweet pepper (Capsicum annuum L.) grafted onto different commercial rootstocks. Biological Agriculture and Horticulture 29: 1–11.CrossRefGoogle Scholar
López-Marín, J, González, A, Pérez-Alfocea, F, Egea-Gilabert, C and Fernández, JA (2013) Grafting is an efficient alternative to shading screens to alleviate thermal stress in greenhouse-grown sweet pepper. Scientia Horticulturae 149: 39–46.CrossRefGoogle Scholar
Penella, C, Nebauer, SG, López-Galarza, S, SanBautista, A, Gorbe, E and Calatayud, A (2013) Evaluation for salt stress tolerance of pepper genotypes to be used as rootstocks. Journal of Food, Agriculture and Environment 11: 1101–1107.Google Scholar
Robertson, L, López-Pérez, JA, Bello, A, Díez-Rojo, MA, Escuer, M, Piedra-Buena, A, Ros, C and Martínez, C (2006) Characterization of Meloidogyne incognita, M. arenaria and M. hapla populations from Spain and Uruguay parasitizing pepper (Capsicum annuum). Crop Protection 25: 440–445.Google Scholar
Ros, C, Guerrero, MM, Martínez, MA, Barceló, N, Martínez, MC, Rodríguez, I, Guirao, P, Bello, A and Lacasa, A (2005) Resistant sweet pepper rootstocks integrated into the management of soilborne pathogens in greenhouse. Acta Horticulturae 698: 305–310.Google Scholar
Ros, C, Lacasa, CM, Martínez, V, Bielza, P and Lacasa, A (2014) Response of pepper rootstocks to co-infection of Meloidogyne incognita and Phytophthora spp. European Journal of Horticultural Science 79: 22–28.Google Scholar
Ros-Ibáñez, C, Robertson, L, Martínez-Lluch, MC, Cano-García, A and Lacasa-Plasencia, A (2014) Development of virulence to Meloidogyne incognita on resistant pepper rootstocks. Spanish Journal of Agricultural Research 12: 225–232.CrossRefGoogle Scholar
Sánchez, F, Hernández, A, Lacasa, CM, Martínez, V, Guerrero, MM, Sánchez, E, Gomariz, J, Ros, C and Lacasa, A (2013) Pepper rootstocks: agronomic evaluation and behaviour against Meloidogyne incognita and Phytophthora spp. in greenhouses of Murcia (Spain). In: Lanteri, S and Rotino, GL (eds) Breakthroughs in the Genetics and Breeding of Capsicum and Eggplant. Torino: Comitato per l'organizzazione degli eventi (COE) DIFASA, Università degli Studi di Torino, pp. 449–452.Google Scholar
Schwarz, D, Rouphael, Y, Colla, G and Venema, JH (2010) Grafting as a tool to improve tolerance of vegetables to abiotic stresses: thermal stress, water stress and organic pollutants. Scientia Horticulturae 127: 162–171.Google Scholar
Talavera, M, Sayadi, S, Chirrosa-Ríos, M, Salmerón, T, Flor-Peregrín, E and Verdejo-Lucas, S (2012) Perception of the impact of root-knot nematode-induced diseases in horticultural protected crops of south-eastern Spain. Nematology 14: 517–527.Google Scholar
Thies, JA (2011) Virulence of Meloidogyne incognita to expression of N gene in pepper. Journal of Nematology 43: 90–94.Google Scholar
Thies, JA and Fery, RL (2003) Response of bell pepper cultivars near-isogenic for the N gene to Meloidogyne incognita in field trials. HortScience 38: 1394–1396.CrossRefGoogle Scholar