Hostname: page-component-848d4c4894-tn8tq Total loading time: 0 Render date: 2024-06-23T00:36:34.107Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of drought stress tolerance in wild species germplasm of rice based on leaf and root morphology

Published online by Cambridge University Press:  30 October 2017

Kumari Neelam ,
Gurpreet K. Sahi ,
Kishor Kumar  and
Kuldeep Singh
Kumari Neelam*
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, India
Gurpreet K. Sahi
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, India
Kishor Kumar
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, India
Kuldeep Singh
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, India ICAR – National Bureau of Plant Genetic Resources, New Delhi, 110073, India
*
*Corresponding author. E-mail: kneelam@pau.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

Drought is the major abiotic constraint to the rice production in the rain-fed areas across Asia and sub-Saharan Africa. Wild species of Oryza offer a wide spectrum of adaptive traits and can serve as potential donors of biotic and abiotic stress tolerance. At the Punjab Agricultural University, we are maintaining an active collection of 1630 accessions of wild species germplasm (AA, CC, BBCC and CCDD) of rice. These accessions were screened to assess genetic variation for drought tolerance under field conditions. Severe water stress was imposed at the late vegetative stage by withholding water initially for 25 d and then extended further to 35 d during kharif season in the years 2013–14 and 2015–16. The tolerance score for drought stress was based on the extent of leaf rolling and leaf drying. Based on the 2 years’ data, seven accessions from Oryza rufipogon, four from Oryza longistaminata and one each from Oryza officinalis and Oryza latifolia were found tolerant to drought stress. These selected accessions were further phenotype for root morphology. The average root length among the selected accessions ranges between 36 and 80 cm and the number of primary roots vary from 30 to 87 cm. The O. rufipogon accession IRGC 106433, O. longistaminata accession IRGC 92656A, O. officinalis accession IRGC 101152 and O. latifolia accession IRGC 80769 showed approximately 2–2.5 times longer root length and number than the indica rice elite cultivar PR121. The results indicated potentiality of selected wild species germplasm for conferring drought tolerance to the elite cultivars.

Keywords

abiotic stressdrought toleranceleaf rollingroot morphologywild species germplasm of rice
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 4 , August 2018 , pp. 289 - 295
Copyright
Copyright © NIAB 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Biswal, AK and Kohli, A (2013) Cereal flag leaf adaptations for grain yield under drought: knowledge status and gaps. Molecular Breeding 31: 749–766.Google Scholar
Blum, A (1988) Plant Breeding for Stress Environments. Florida, USA: CRC. Inc.Google Scholar
Brar, DS and Khush, GS (1997) Alien introgression in rice. Plant Molecular Biology 35: 35–47.Google Scholar
Brar, DS and Singh, K (2011) Oryza. In: Kole, C (ed.) Wild Crop Relatives: Genomic and Breeding Resources, Cereals. Berlin: Springer, pp. 321–365.Google Scholar
Cerqueira, FB, Erasmo, EAL, Silva, JIC, Nunes, TV, Carvalho, GP and Silva, AA (2013) Competition between drought-tolerant upland rice cultivars and weeds under water stress condition. Planta Daninha 31: 291–302.Google Scholar
Datta, SK, Malabuyoc, JA and Aragon, EL (1988) A field screening technique for evaluating rice germplasm for drought tolerance during vegetative stage. Field Crops Research 19: 123–124.Google Scholar
Ding, XP, Li, XK and Xiong, LZ (2011) Evaluation of near-isogenic lines for drought resistance QTL and fine mapping of a locus affecting flag leaf width, spikelet number, and root volume in rice. Theoretical Applied Genetics 123: 815–826.Google Scholar
Farooq, M, Wahid, A and Lee, DJ (2009) Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiologia Plantarum 31: 937–945.Google Scholar
Feng, F, Xu, X, Du, X, Tong, H, Luo, L and Mei, H (2012) Assessment of drought resistance among wild rice accessions using a protocol based on single tiller propagation and PVC-tube cultivation. Australian Journal of Crop Science 6: 1204–1211.Google Scholar
Kumar, S, Dwivedi, SK, Singh, SS, Bhatt, BP, Mehta, P, Elanchezhian, R, Singh, VP and Singh, ON (2014) Morphophysiological traits associated with reproductive stage drought tolerance of rice (Oryza sativa L.) genotypes under rain-fed condition of eastern Indo-Gangetic Plain. Indian Journal of Plant Physiology 19: 87–93.Google Scholar
Liu, L, Lafitte, R and Guan, D (2004) Wild Oryza species as potential sources of drought adaptive traits. Euphytica 138: 149–161.Google Scholar
Loresto, GC and Chang, TT (1981). Decimal scoring system for drought reactions and recovery ability in screening nurseries of rice. International Rice Research Newsletter, 6(2): 9–10.Google Scholar
Nguyen, TTT, Klueva, N, Chamareck, V, Aarti, A, Magpantaya, , Millena, ACM and Pathan, MS (2004) Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Molecular Genetics Genomics 272: 35–46.Google Scholar
Salunkhe, AS, Poornima, R, Prince, KS, Kanagaraj, P, Sheeba, JA, Amudha, K, Suji, KK, Senthil, A and Babu, RC (2011) Fine mapping QTL for drought resistance traits in rice (Oryza sativa L.) using bulk segregant analysis. Molecular Biotechnology 49: 90–95.Google Scholar
Samson, BK, Hasan, H and Wade, LJ (2002) Penetration of hardpans by rice lines in the rainfed lowlands. Field Crop Research 76: 175–188.Google Scholar
Singh, A, Sengar, K and Sengar, RS (2013) Gene regulation and biotechnology of drought tolerance in rice. International Journal of Biotechnology and Bioengineering Research 4: 547–552.Google Scholar
Singh, K, Neelam, K, Kaur, A and Kaur, K (2016a) Rice. In: Singh, M, Kumar, S (eds) Broadening the Genetic Base of Grain Cereals. Berlin: Springer, pp. 27–65.Google Scholar
Singh, R, Singh, Y, Xalaxo, S, Verulkar, S, Yadav, N, Singh, S, Singh, N, Prasad, KSN, Kondayya, K, Ramana Rao, PV, Girija Rani, M, Anuradha, T, Suraynarayana, Y, Sharma, PC, Krishnamurthy, SL, Sharma, SK, Dwivedi, JL, Singh, AK, Singh, PK, Nilanjay, , Singh, NK, Kumar, R, Chetia, SK, Ahmad, T, Rai, M, Perraju, P, Pande, A, Singh, DN, Mandal, NP, Reddy, JN, Singh, ON, Katara, JL, Marandi, B, Swain, P, Sarkar, RK, Singh, DP, Mohapatra, T, Padmawathi, G, Ram, T, Kathiresan, RM, Paramsivam, K, Nadarajan, S, Thirumeni, S, Nagarajan, M, Singh, AK, Vikram, P, Kumar, A, Septiningshih, E, Singh, US, Ismail, AM, Mackill, D and Singh, NK (2016b) From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network. Plant Science 242: 278–287.Google Scholar
Sokoto, MB and Muhammad, A (2014) Response of rice varieties to water stress in Sokoto, Sudan Savannah, Nigeria. Journal of Biosciences and Medicines 2: 68–74.Google Scholar
Subashri, M, Robin, S, Vinod, KK, Rajeswari, S, Mohanasundaram, K and Raveendran, TS (2009) Trait identification and QTL validation for reproductive stage drought resistance in rice using selective genotyping of near flowering RILs. Euphytica 166: 291–305.Google Scholar
Uga, Y, Sugimoto, K, Ogawa, S, Rane, J, Ishitani, M, Hara, N, Kitomi, Y, Inukai, Y, Ono, K, Kanno, N, Inoue, H, Takehisa, H, Motoyama, R, Nagamura, Y, Wu, J, Matsumoto, T, Takai, T, Okuno, K and Yano, M (2013) Control of root system architecture by DEEPER ROOTING 1 increases rice yield under drought conditions. Nature Genetics 45: 1097–1102.Google Scholar
Wang, H and Yamauchi, A (2006) Growth and function of roots under abiotic stress in soil. In: Huang, BR (ed.) Plant-Environment Interactions, 3rd edn. New York: CRC Press, pp. 271–320.Google Scholar
Wopereis, MCS, Kropff, MJ, Maligaya, AR and Tuong, TP (1996) Drought-stress responses of two lowland rice cultivars to soil water status. Field Crops Research 46: 21–39.Google Scholar
Zhang, JW, Long, Y, Xue, MD, Xiao, XG and Pei, XW (2017) Identification of microRNAs in response to drought in common wild rice (Oryza rufipogon Griff.) shoots and roots. PLoS ONE 12: e0170330. doi: 10.1371/journal.pone.0170330.Google Scholar
Zhang, X, Zhou, S, Fu, Y, Su, Z, Wang, X and Sun, C (2006) Identification of a drought tolerant introgression line derived from Dongxiang common wild rice (O. rufipogon Griff.). Plant Molecular Biology 62: 247–259.Google Scholar
Supplementary material: Image

Neelam et al supplementary material

Figure S1

Download Neelam et al supplementary material(Image)
Image 1.3 MB
Supplementary material: File

Neelam et al supplementary material

Tables S1-S3

Download Neelam et al supplementary material(File)
File 24 KB