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Genetic relationships among napiergrass (Pennisetum purpureum Schum.) nursery accessions using AFLP markers

Published online by Cambridge University Press:  22 September 2009

Karen Harris ,
William Anderson  and
Ravindra Malik
Karen Harris*
Affiliation:
Crop Genetics and Breeding Research Unit, USDA–ARS, 115 Coastal Way, Tifton, GA31793, USA
William Anderson
Affiliation:
Crop Genetics and Breeding Research Unit, USDA–ARS, 115 Coastal Way, Tifton, GA31793, USA
Ravindra Malik
Affiliation:
Department of Natural Science, Albany State University, ACAD 316, 504 College Drive, Albany, GA31705, USA
*
* Corresponding author. E-mail: karen.harris@ars.usda.gov
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Abstract

Pennisetum purpureum Schum. (napiergrass) is a perennial grass used for forage especially in South America and Africa. Over the last 30 years, a USDA–ARS nursery containing accessions collected from all over the world has been established in Tifton, Georgia. The study reported here was conducted to assess the molecular genetic variation and genetic relatedness among 89 accessions from the Tifton nursery using amplified fragment length polymorphism markers, morphological data and ploidy level. Using 218 polymorphic markers from eight selective primer combinations, the 89 accessions were clustered into five groups using a principal components analysis and a dendrogram based on Dice similarity estimates and unweighted pair group method with arithmetic average clustering. These five groups include three groups collected from Kenya, a group from Puerto Rico, and accessions derived from the cultivar Merkeron. This research provides the first molecular characterization of the Tifton nursery, displays the relationships between accessions, and provides potential heterotic groups for napiergrass and pearl millet (Pennisetum glaucum (L.) R. Br.) breeding improvement.

Keywords

AFLPDiceGenetic similarityPCAUPGMA
Type
Research Article
Information
Plant Genetic Resources , Volume 8 , Issue 1 , April 2010 , pp. 63 - 70
Copyright
Copyright © NIAB 2009

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