Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-04-30T19:51:30.825Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of French bean germplasm from Garhwal Himalayas for resistance to angular leaf spot

Published online by Cambridge University Press:  08 March 2023

Navneeti Chamoli ,
Deepti Prabha Open the ORCID record for Deepti Prabha [Opens in a new window] ,
Yogesh Kumar Negi  and
Jai Singh Chauhan
Navneeti Chamoli
Affiliation:
Department of Seed Science & Technology, School of Agriculture and Allied Sciences, HNB Garhwal University, Srinagar, Uttarakhand, India
Deepti Prabha*
Affiliation:
Department of Seed Science & Technology, School of Agriculture and Allied Sciences, HNB Garhwal University, Srinagar, Uttarakhand, India
Yogesh Kumar Negi
Affiliation:
Department of Basic Sciences, College of Forestry, (VCSG UUHF), Ranichauri, Tehri Garhwal, Uttarakhand, India
Jai Singh Chauhan
Affiliation:
Department of Seed Science & Technology, School of Agriculture and Allied Sciences, HNB Garhwal University, Srinagar, Uttarakhand, India
*
Author for correspondence: Deepti Prabha, E-mail: deepti_prabha@rediffmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Angular leaf spot (ALS) caused by Pseudocercospora griseola is a major disease of french bean (Phaseolus vulgaris L.) worldwide. A good diversity of French bean is present in the Garhwal Himalayas of Uttarakhand, India, which is unexplored. The purpose of this study was to identify ALS-resistant accessions among local landraces of French bean in this region. One hundred seventy-six local accessions were collected from different villages of Garhwal, Uttarakhand. All the accessions were screened by four SCAR primers SN02 (Phg-2), SAA19, SM02, SBA16 (Phg-3), one STS primer TGA1.1 (Phg-1) and one SSR primer Pv-at006 (Phg-5). All the accessions were also screened for ALS resistance under field condition in the years 2019 and 2020. The disease-resistant score was recorded on 1–9 scale. After field screening, 48 accessions (19 resistant, 24 moderately resistant and five susceptible) were selected for in-vitro screening under screen house condition. These 46 accessions were artificially inoculated by two different isolates of P. griseola P5 and P9, which are the most virulent pathotype characterized by microbiology lab, College of Forestry, Tehri, Uttarakhand. After in-vitro screening, seven accessions (GFB-25, GFB-26, GFB-30, GFB-32, GFB-93, GFB-97 and GFB-136) were found resistant to both the isolates P5 and P9. The P. griseola-resistant accessions may further be used in future breeding programmes to develop new and more resistant varieties of French bean against ALS.

Keywords

ALSdisease reactionFrench beanmolecular markerPhg genes
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 4 , August 2022 , pp. 255 - 262
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Almeida, CP, de Carvalho, PJF, Bonfante, GFJ, Perseguini, JMKC, Santos, IL, Gonçalves, JGR, Patricio, FRA, Taniguti, CH, Gesteira, G, Garcia, AAF, Song, Q, Carbonell, SAM, Chiorato, AF and Benchimol-Reis, LL (2021) Angular leaf spot resistance loci associated with different plant growth stages in common bean. Frontiers in Plant Sciences 12, 647043.CrossRefGoogle ScholarPubMed
Balardin, RS, Jarosz, AM and Kelly, JD (1997) Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central and North America. Phytopathology 87, 1184–1191.CrossRefGoogle ScholarPubMed
Bassi, D, Brinez, B, Rosa, JS, Oblessuc, PR, Almeida, CP and Nucci, SM (2017) Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans. Genetics and Molecular Biology 40, 109–122.CrossRefGoogle ScholarPubMed
Broughton, WJ, Hernandez, G, Blair, M, Beebe, S, Gepts, P and Vanderleyden, J (2003) Beans (Phaseolus spp) – model food legumes. Plant and Soil 252, 55–128.CrossRefGoogle Scholar
Busogoro, JP, Duterme, O and Lepoivre, P (2002) Development of microsatellite markers for the characterisation of Phaeoisariopsis griseola (bean angular leaf spot agent) populations in central America. Plant Protection Science 38, 35–37.CrossRefGoogle Scholar
Caixeta, EF, Borem, A, de Morais Silvia, NG, Rocha, RC, de Barros, EG and Moreira, MA (2002) Teste de alelismo para genes do feijoeiro que conferem resistencia ao fungo Phaeoisariopsis griseola. VII Congresso Nacional de Pesquisa de Jeijiao. Vicosa MG, Brazil: Universidade Federal de Vicosa.Google Scholar
Caixeta, ET, Borem, A, Alzate-Marin, AL, Fagundes, SDA, Silva, MGDME, De Barros, EG and Moreira, MA (2005) Allelic relationships for genes that confer resistance to angular leaf spot in common bean. Euphytica 145, 237–245.CrossRefGoogle Scholar
Carvalho, GA, Paula Junior, TJ, Alzate-Marin, AL, Nietsche, S, Barros, EG and Moreira, MA (1998) Inheritance of resistance of the Andean bean line AND-277 to race 63-23 of Phaeoisariopsis griseola and identification of a RAPD marker linked to the resistance gene. Fitopatologia Brasileira 23, 482–485.Google Scholar
Correa, RX, Good-God, PIV, Oliveira, MLP, Nietsche, S, Moreira, MA and Barros, EGDE (2001) Inheritance of resistance to the common bean angular leaf spot and identification of molecular markers flanking the resistance locus. Fitopatologia Brasileira 26, 27–32.Google Scholar
Crous, PW, Liebenberg, MM, Braun, U and Groenewald, JZ (2006) Re-evaluating the taxonomic status of Phaeoisariopsis griseola the causal agent of angular leaf spot of bean. Journal of Studies in Mycology 55, 163–173.CrossRefGoogle ScholarPubMed
Ddamulira, G, Mukankusi, C, Ochwo, M, Edema, R, Sseruwagi, P and Gepts, P (2015) Gene pyramiding improved resistance to angular leaf spot in common bean. American Journal of Experimental Agriculture 9, 1–12.CrossRefGoogle Scholar
del Rio, LE, Lamppa, RS, Gross, PL, Brolley, B and Prischmann, J (2003) Identification of Colletotrichum lindemuthianum race 73 in Manitoba, Canada. Canadian Journal of Phytopathology 25, 104–107.Google Scholar
Devi, KD, Punyarani, K, Sing, NS and Devi, HS (2013) An efficient protocol for total DNA extraction from the members of order Zingiberales – suitable for diverse PCR based downstream applications. Springer Plus 2, 669.CrossRefGoogle ScholarPubMed
Gil, J, Solarte, D, Lobaton, JD, Mayor, V, Barrera, S and Jara, C (2019) Fine-mapping of angular leaf spot resistance gene Phg-2 in common bean and development of molecular breeding tools. Theoretical and Applied Genetics 132, 2003–2016.CrossRefGoogle ScholarPubMed
Gonçalves-Vidigal, MC, Cruz, AS, Garcia, A, Kami, J, Vidigal Filho, PS and Sousa, LL (2011) Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND 277. Theoretical and Applied Genetics 122, 893–903.CrossRefGoogle Scholar
Gonçalves-Vidigal, MC, Cruz, AS, Lacanallo, GF, Vidigal, PS, Sousa, LL and Pacheco, CMNA (2013) Cosegregation analysis and mapping of the Anthracnose Co-10 and angular leaf spot Phg-ON disease-resistance genes in the common bean cultivar Ouro Negro. Theoretical and Applied Genetics 126, 2245–2255.CrossRefGoogle ScholarPubMed
Gulsum, P, Goksel, O, Mehmet, ZY, Vahdettin, C and Harun, B (2021) Resistance sources and reactions of common bean (Phaseolus vulgaris L.) cultivars in Turkey to anthracnose disease. Genetic Resources and Crop Evolution 68. doi: 10.1007/s10722-021-01195-4(0123456789)Google Scholar
Guzman, PRL, Gilbertson, RO, Nodari, WCJ, Temple, SR and Mandala, D (1995) Characterization of variability in the fungus Phaeoisariopsis griseola suggests coevolution with the common bean (Phaseolus vulgaris). Phytopathology 85, 600–607.CrossRefGoogle Scholar
Ibarra-Perez, FJ, Ehdaie, B and Waines, JG (1997) Estimation of outcrossing rate in common bean. Crop Science 37, 60–65.CrossRefGoogle Scholar
Keller, B, Manzanares, C, Jara, C, Lobaton, JD, Studer, B and Raatz, B (2015) Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 128, 813–826.CrossRefGoogle Scholar
Kelly, JD, Afanador, L and Cameron, LS (1994) New races of Colletotrichum lindemuthianum in Michigan and implications in dry bean resistance breeding. Plant Disease 78, 892–894.CrossRefGoogle Scholar
Librelon, SS, de-Pádua, PF, de-Fátima, BAA, Ramalho, MAP and de-Souza, EA (2020) Increasing the efficiency of recurrent selection for angular leaf spot resistance in common bean. Crop Science 60, 751–758.CrossRefGoogle Scholar
Lopez, CE, Acosta, IF, Jara, C, Pedraza, F, Gaitan-Solis, E and Gallego, G (2003) Identifying resistance gene analogs associated with resistances to different pathogens in common bean. Phytopathology 93, 88–95.CrossRefGoogle ScholarPubMed
Mahuku, GS, Jara, C, Cuasquer, JB and Castellanos, G (2002) Genetic variability within Phaeoisariopsis griseola from Central America and its implications for resistance breeding of common bean. Plant Pathology 51, 594–604.CrossRefGoogle Scholar
Mahuku, GS, Montoya, C, Henriquez, MA, Jara, C, Teran, H and Beebe, S (2004) Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene. Crop Science 44, 1817–1824.CrossRefGoogle Scholar
Marin, AL, Costa, MR, Arruda, KM, Barros, EG and Moreira, MA (2003) Characterization of the anthracnose resistance gene present in Ouro Negro (Honduras 35) common bean cultivar. Euphytica 133, 165–169.CrossRefGoogle Scholar
Miklas, PN, Pastor-Corrales, MA, Jung, G, Coyne, DP, Kelly, JD, McClean, PE and Gepts, P (2002) Comprehensive linkage map of bean rust resistance genes. Annual Report of the Bean Improvement Cooperative 45, 125–129.Google Scholar
Nay, MM, Souza, TLPO, Raatz, B, Mukankusi, CM, Gonçalves-Vidigal, MC, Abreu, AFB, Melo, LC and Pastor-Corrales, AM (2019) A review of angular leaf spot resistance in common bean. Crop science 59, 1376–1391.CrossRefGoogle ScholarPubMed
Nietsche, S, Borem, A, Carvalho, GA, Rocha, RC, Paula-Junior, TJ, Barros, EG and Moreira, MA (2000) RAPD and SCAR markers linked to a gene conferring resistance to angular leaf spot in common bean. Phytopathology 148, 117–121.CrossRefGoogle Scholar
Oblessuc, P, Baroni, RM, Garcia, AAF, Chioratto, AF, Carbonell, SAM, Camargo, LEA and Benchimol, LL (2012) Mapping of angular leaf spot resistance QTL in common bean (Phaseolus vulgaris L.) under different environments. BMC Genetics 13, 50.CrossRefGoogle ScholarPubMed
Oblessuc, PR, Perseguini, JMKC, Baroni, RM, Chiorato, AF, Carbonell, SAM and Mondego, JMC (2013) Increasing the density of markers around a major QTL controlling resistance to angular leaf spot in common bean. Theoretical and Applied Genetics 126, 2451–2465.CrossRefGoogle Scholar
Pastor-Corrales, MA and Jara, CE (1995) The evolution of Phaeoisariopsis griseola with the common bean in Latin America. Fitopatologia Colombiana 1, 15–24.Google Scholar
Pastor-Corrales, MA, Jara, C and Singh, SP (1998) Pathogenic variation in, sources of, and breeding for resistance to Phaeoisariopsis griseola causing angular leaf spot in common bean. Euphytica 103, 161–171.CrossRefGoogle Scholar
Pastor-Corrales, MA, Rayapati, J, Osorno, JM, Kelly, JD, Wright, EM and Brick, MAG (2010) Reaction of common bean cultivars to two new races of the rust pathogen from Michigan and North Dakota. Annual Report of the Bean Improvement Cooperative 53, 64–65.Google Scholar
Paula-Junior, TJ and Zambolim, L (1998) Doencas. In Vieria, C, Paula-Junior, TJ and Borem, A (eds), Feijao: aspectosgerais e cultura no Estado de Minas. Vicosa: Editora UFV, pp. 375–433.Google Scholar
Pedro, W, Merion, C, Liebenberg, M, Braun, U and Groenewald, JZ (2006) Re-evaluating the taxonomic status of Phaeoisariopsis griseola, the causal agent of angular leaf spot of bean. Studies in Mycology 55, 163–173.Google Scholar
Pereira, R, Abreu, FB, Nalin, ARS and Souza, EA (2019) Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance. Scientia Agricola 76, 415–423.CrossRefGoogle Scholar
Perseguini, JMKC, Oblessuc, PR, Rosa, JRBF, Gomes, KA, Chiorato, AF and Carbonell, SAM (2016) Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). PLoS ONE 11, e0150506.CrossRefGoogle ScholarPubMed
Prabha, D, Chamoli, N, Negi, YK and Chauhan, JS (2021) Multiple genes confer anthracnose resistance in French bean (Phaseolus vulgaris L.) accessions of Garhwal Himalayas. Genetic Resources and Crop Evolution 69, 809–821. https://doi.org/10.1007/s10722-021-01266-6CrossRefGoogle Scholar
Queiroz, VT, Sousa, CS, Costa, MR, Sanglad, DA, Arruda, KMA and Souza, TLPO (2004) Development of SCAR markers linked to common bean angular leaf spot resistance genes. Annual Report of the Bean Improvement Cooperative 47, 237–238.Google Scholar
Ramalho, MAP and Abreu, AFB (2006) Cultivares. In Vieira, C, Paula Junior, TJ and Borem, A (eds), Feijão, 2nd Edn. Viçosa: UFV, pp. 415–436.Google Scholar
Sanglard, DA, Ribeiro, CAG, Balbi, BP, Arruda, KMA, De-Barros, EG and Moreira, MA (2013) Characterization of the angular leaf spot resistance gene present in common bean cultivar Ouro Negro. Journal of Agricultural Science 5, 19–23.CrossRefGoogle Scholar
Sartorato, A, Nietsche, S, Barros, EG and Moreira, MA (1999 a) SCAR marker linked to angular leaf spot resistance gene in common bean. Annual Report of the Bean Improvement Cooperative 42, 23–24.Google Scholar
Sartorato, A, Nietsche, S, Barros, EG and Moreira, MA (1999 b) Inheritance of angular leaf spot resistance and RAPD markers linked to disease resistance gene in common beans. Annual Report of the Bean Improvement Cooperative 42, 21–22.Google Scholar
Souza, TLPO, Dessaune, SN, Sanglard, DA, Moreira, MA and de Barros, EG (2011) Characterization of the rust resistance gene present in the common bean cultivar Ouro Negro, the main rust resistance source used in Brazil. Plant Pathology 60, 839–845.CrossRefGoogle Scholar
Tryphone, GM, Chilagane, LA, Nchimbi-Msolla, S and Kusolwa, PM (2015) Genetic characterization of angular leaf spot resistance in selected common bean landraces from Tanzania. African Journal of Biotechnology 14, 2943–2948.Google Scholar
Supplementary material: File

Chamoli et al. supplementary material

Table S1

Download Chamoli et al. supplementary material(File)
File 37.2 KB