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Newly developed SNP markers related to genes for leaf morphological traits and disease resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Published online by Cambridge University Press:  16 July 2014

Yul-Kyun Ahn*
Affiliation:
Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Republic of Korea
Young-Il Cho
Affiliation:
Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Republic of Korea
Swati Tripathi
Affiliation:
Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Republic of Korea
Do-Sun Kim
Affiliation:
Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Republic of Korea
Jeong-Ho Kim
Affiliation:
Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Republic of Korea
Hye-Eun Lee
Affiliation:
Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Republic of Korea
*
* Corresponding author. E-mail: aykyun@korea.kr

Abstract

Based on the sequences of Brassica rapa ssp. pekinensis, we carried out a single-nucleotide polymorphism (SNP) study for genes controlling disease resistance (clubroot, turnip mosaic virus and soft rot) and leaf traits in B. rapa leafy genotypes ‘VC40’ and ‘SR5’. In total, 7645 SNP markers were obtained based on the annotation of a B. rapa database for disease resistance. Among these 7645 SNP markers, 141 were related to 125 genes linked to leaf traits. From these 141 SNP markers, 63 were screened and High Resolution Melt (HRM) primers were designed for genomic studies. A total of 20 polymorphic SNP primers were finally obtained. Eventually, these markers will be further used for the detection of quantitative trait loci and mapping studies.

Type
Research Article
Copyright
Copyright © NIAB 2014 

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Supplementary material: File

Ahn Supplementary Material

Table S1

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Newly developed SNP markers related to genes for leaf morphological traits and disease resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
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Newly developed SNP markers related to genes for leaf morphological traits and disease resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
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