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Morphological and molecular characterization of underutilized medicinal wild ginger (Zingiber barbatum Wall.) from Myanmar

Published online by Cambridge University Press:  29 July 2011

Noladhi Wicaksana ,
Syed Abdullah Gilani ,
Dawood Ahmad ,
Akira Kikuchi  and
Kazuo N. Watanabe
Noladhi Wicaksana
Affiliation:
Watanabe Laboratory, Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki305-8572, Japan Plant Breeding Laboratory, Faculty of Agriculture, Padjadjaran University, Bandung40600, Indonesia
Syed Abdullah Gilani*
Affiliation:
Watanabe Laboratory, Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki305-8572, Japan
Dawood Ahmad
Affiliation:
Institute of Bio-Technology and Genetics Engineering, Khyber Pakhtunkhwa Agricultural University, Peshawar, Pakistan
Akira Kikuchi
Affiliation:
Watanabe Laboratory, Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki305-8572, Japan
Kazuo N. Watanabe
Affiliation:
Watanabe Laboratory, Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki305-8572, Japan
*
*Corresponding author. E-mail: gilani_abdullah@yahoo.com
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Abstract

Zingiber barbatum Wall. (family Zingiberaceae), is an underutilized medicinal plant and commonly known as ‘Meik tha-lin’ in Myanmar where it is used in the indigenous system of medicine. In the present study, 19 accessions of Z. barbatum from five provinces in Myanmar have been utilized to characterize and assess genetic diversity. Twenty-nine morphological characters were noted, including growth habit, leaf, pseudo-stem and rhizome characters. Fifteen primer sets of P450-based analogue (PBA) markers were used to reveal molecular characteristics. Of the 29 morphological characters, 22 showed a high degree of variation within wild ginger accessions, whereas 20 of these characters contributed significantly to morphological variation. Eleven amplified primer sets gave a total of 175 bands and exhibited 92.15% polymorphism across Z. barbatum accessions. Based on morphological characters and PBA markers, 19 accessions can be divided into two morphotype groups with comparatively higher genetic diversity. This information can be applied in future crop improvement, proper conservation and better use of this underutilized medicinal species.

Keywords

genetic diversitymorphological and genetic characterizationwild gingerZingiber barbatumZingiberaceae
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 4 , December 2011 , pp. 531 - 542
Copyright
Copyright © NIAB 2011

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