Hostname: page-component-7479d7b7d-qs9v7 Total loading time: 0 Render date: 2024-07-12T15:57:13.489Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of stable sources of resistance to mungbean yellow mosaic virus (MYMV) disease in mungbean [Vigna radiata (L.) Wilczek]

Published online by Cambridge University Press:  27 May 2019

Nagaraj ,
S Basavaraj ,
A.S. Padmaja ,
N Nagaraju  and
S Ramesh Open the ORCID record for S Ramesh [Opens in a new window]
Nagaraj
Affiliation:
Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra, Bengaluru, India
S Basavaraj
Affiliation:
Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra, Bengaluru, India
A.S. Padmaja
Affiliation:
Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra, Bengaluru, India
N Nagaraju*
Affiliation:
Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra, Bengaluru, India
S Ramesh
Affiliation:
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru, India
*
*Corresponding author. E-mail: nagaraju63kgere@yahoo.co.in
Get access Rights & Permissions [Opens in a new window]

Abstract

Yellow mosaic disease (YMD) caused by mungbean yellow mosaic virus (MYMV) is one of the most destructive biotic production constraints in mungbean. Development and introduction of resistant cultivars are considered as the most economical and eco-friendly option to manage YMD, for which availability of stable sources of resistance is a pre-requisite. A set of 14 mungbean genotypes including a susceptible check were evaluated for responses to YMD under natural infection across three seasons and under challenged inoculation in glasshouse for one season. None of the genotypes were immune to YMD and produced different degrees of response to MYMV in terms of yellow mosaic symptoms (YMS). Based on the delayed appearance of initial YMS, and lower estimates of per cent disease index and area under disease progressive curve (AUDPC) in response to natural infection and challenged inoculation, five genotypes namely AVMU 1698, AVMU 1699, AVMU 16100, AVMU 16101 and KPS 2 were identified as resistant to YMD. Failure of detection of MYMV through polymerase chain reaction (PCR) using MYMV coat protein gene-specific primer and successful detection of the same through rolling circle amplification-PCR suggested latent infection of MYMV in resistant genotypes. The resistance response of the five genotypes could be attributed to enhanced activities of enzymes such as peroxidase, polyphenol oxidase and phenylalanine ammonia lyase and increased concentration of total phenols. These results are discussed in relation to strategies to breed mungbean for resistance to YMD.

Keywords

mungbean yellow mosaic virusPCRresistanceyellow mosaic symptoms
Type
Research Article
Information
Plant Genetic Resources , Volume 17 , Issue 4 , August 2019 , pp. 362 - 370
Copyright
Copyright © NIAB 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akhtar, KP, Kitsanachandee, R, Srinives, P, Abbas, G, Asghar, MJ, Shah, TM, Atta, BM, Chatchawankanphanich, O, Sarwar, G, Ahmad, M and Sarwar, N (2009) Field evaluation of mungbean recombinant inbred lines against mungbean yellow mosaic disease using new disease scale in Thailand. Journal of Plant Pathology 25: 422–428.10.5423/PPJ.2009.25.4.422Google Scholar
Akhtar, KP, Sarwar, G, Abbas, G, Asghar, MJ, Sarwar, N and Shah, TM (2011) Screening of mungbean germplasm against mungbean yellow mosaic India virus and its vector Bemisia tabaci. Crop Protection 30: 1202–1209.10.1016/j.cropro.2011.05.012Google Scholar
Ammavasai, S, Phogat, DS and Solanki, IS (2004) Inheritance of resistance to mungbean yellow mosaic virus (MYMV) in green gram (Vigna radiata L. Wilczek). Indian Journal of Genetics 64: 145–146.Google Scholar
Ashwathnarayana, DS, Shankarappa, KS, Prameela, HA, Raghavendra, N, Keshava murthy, KV and Rangaswamy, KT (2005) New hosts of begomoviruses in Karnataka. In: Second Global Conference: Plant health, Global wealth, Udaipur, pp. 14.Google Scholar
Bora, A, Gogoi, HK and Kalita, MC (2016) Use of RCA-PCR assay for detection of Begomovirus infection in Bhut jolokia (Capsicum assamicum) in Tezpur region of Assam, India. South Asian Journal of Experimental Biology 6: 64–69.Google Scholar
Bowles, DJ (1990) Defense-related proteins in higher plants. Annual Review of Biochemistry 59: 873–907.10.1146/annurev.bi.59.070190.004301Google Scholar
Brown, JK (2007) The Bemisia tabaci complex: genetic and phenotypic variability drives begomovirus spread and virus diversification. doi: 10.1094/APSnetFeature-2007-2010.Google Scholar
Campbell, CL and Madden, LV (1990) Introduction to Plant Disease Epidemiology. New York: Wiley Interscience, p. 532.Google Scholar
Deepa, H, Govindappa, MR, Kulkarni, SA, Kenganal, M and Biradar, SA (2017) Molecular detection of yellow mosaic disease of green gram through polymerase chain reaction. Bulletin of Environment, Pharmacology and Life Sciences 6: 507–509.Google Scholar
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19: 11–15.Google Scholar
Duffy, S and Holmes, EC (2008) Phylogenetic evidence for rapid rates of molecular evolution in the single stranded DNA begomovirus tomato leaf curl virus. Journal of Virology 82: 957–965.10.1128/JVI.01929-07Google Scholar
Farooq, M, Naila, I, Muhammad, NK, Muhammad, S, Rahila, F, Shabir, A, Muhammad, B and Nabeela, I (2018) Evaluation of resistance in mungbean (Vigna radiata (L.) R. Wilczek) germplasm against Mungbean yellow mosaic virus (MYMV) with reference to epidemiological studies. International Journal of Fauna Biological Studies 5: 47–56.Google Scholar
Greathead, AH (1986) Host Plants. In: Bemisia tabaci a literature survey on the cotton whitefly with an annotated 90 Journal of Food Legumes 29(2), 2016 bibliography (Ed. M.J.W. Cock). CAB International Institute of Biological Control, Ascot, UK. pp. 7–25.Google Scholar
Gupta, O and Mishra, M (2014) Field resistance in mungbean and urdbean genotypes against yellow mosaic disease. Journal of Food Legumes 27: 80–81.Google Scholar
Hartee, EF (1955) Haematin compounds. In: Peach, K and Tracey, MN (eds) Modern Methods of Plant Analysis. Springer-Verlag, New York, pp. 197–245.Google Scholar
Jeske, H, Gotthardt, D and Kober, S (2010) In planta cloning of geminiviral DNA: the true Sida micrantha mosaic virus. Journal of Virological Methods 163: 301–308.10.1016/j.jviromet.2009.10.014Google Scholar
Kang, BC, Yeam, I and Jahn, MM (2005) Genetics of plant virus resistance. Annual Review of Phytopathology 43: 581621.10.1146/annurev.phyto.43.011205.141140Google Scholar
Karthikeyan, A, Shobhana, VG, Sudha, M, Raveendran, M, Senthil, N, Pandiyan, M and Nagarajan, P (2014) Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia. International Journal of Pest Management 60: 314–324.10.1080/09670874.2014.982230Google Scholar
Kumar, M, Singh, JK, Dalal, PK and Kumar, A (2017) Biochemical basis of resistance against potato apical leaf curl virus disease in Potato. Ecology, Environment and Conservation 23: 417–421.Google Scholar
Lima, ATM, Sobrinho, RR, Gonza'lez, AJ, Rocha, CS, Silva, SJC, Xavier, CAD, Silva, FN, Duffy, S and Zerbini, FM (2012) Synonymous site variation due to recombination explain higher variability in begomovirus populations infecting non- cultivated hosts. Journal of General Virology 94: 418–431.10.1099/vir.0.047241-0Google Scholar
Lovely, B, Radhadevi, DS and Umamaheswaran, K (2017) Change in biochemical activities in yard long bean [Vignaunguiculata ssp. sesquipedalis (L.) verdc] infected with cowpea mosaic virus and their implication in disease resistance. International Journal of Agricultural Science and Research 7: 89–96.10.24247/ijasroct201713Google Scholar
Maheshwari, R, Panigrahi, G and Angappan, K (2014) Molecular characterization of distinct YMV (yellow mosaic virus) isolates affecting pulses in India with the aid of coat protein gene as a marker for identification. Molecular Biology Report 41: 2635–2644.10.1007/s11033-014-3122-9Google Scholar
Manjunatha, N, Noorulla, H, Anjaneya reddy, B, Archana, S and Manjunath, SH (2015) Detection and characterization of virus causing yellow mosaic disease of redgram (Cajanus cajan L. Millsp) in Karnataka. International Journal of Pure and Applied Bioscience 3: 258–264.Google Scholar
Mayer, AM, Harel, E and Shaul, RB (1965) Assay of catechol oxidase, a critical comparison of methods. Phytochemistry 5: 783–789.10.1016/S0031-9422(00)83660-2Google Scholar
Nair, RM, Götz, M, Winter, S, Giri, RR, Boddepalli, VN, Sirari, A, Bains, TS, Taggar, GK, Dikshit, HK, Aski, M and Boopathi, M (2017) Identification of mungbean lines with tolerance or resistance to yellow mosaic in fields in India where different begomovirus species and different Bemisia tabaci cryptic species predominate. European Journal of Plant Pathology 149: 349–365.10.1007/s10658-017-1187-8Google Scholar
Nariani, TK (1960) Yellow mosaic of mung (Phaseolus aureus L.). Indian Phytopathology 13: 24–29.Google Scholar
Niranjanraj, S, Sarosh, BR and Shetty, HS (2006) Induction and accumulation of polyphenol oxidase activities as implicated in the development of résistance against pearl millet downy mildew disease. Functional Plant Biology 33: 563–571.Google Scholar
Obaiah, S, Bhaskara Reddy, BV, Eswara Reddy, NP and Siva Prasad, Y (2014) Molecular detection of yellow mosaic virus infecting blackgram (Vigna mungo (l.) Hepper) in Andhra Pradesh. International Journal of Plant, Animal and Environmental Science 4: 2231–2240.Google Scholar
Pico, B, Diez, M and Nuez, F (1998) Evaluation of whitefly-mediated inoculation techniques to screen Lycopersicon esculentum and wild relatives for resistance to ToLCV. Euphytica 101: 259–277.10.1023/A:1018353806051Google Scholar
Prasanna, HC, Kanakala, S, Archana, K, Jyothsna, P, Varma, RK and Malathi, VG (2015) Cryptic species composition and genetic diversity within Bemisia tabaci complex in soybean in India revealed by mtCOI DNA sequence. Journal of Integrative Agriculture 14: 1786–1795.10.1016/S2095-3119(14)60931-XGoogle Scholar
Reddy, KR and Singh, DP (1995) Inheritance of resistance to mungbean yellow mosaic virus. Madras Agricultural Journal 88: 199–201.Google Scholar
Richert-Pöggeler, KR and Mináróvits, J (2014) Diversity of latent plant-virus interactions and their impact on the virosphere. In: Plant Virus-Host Interaction: Molecular Approaches and Viral Evolution. Elsevier Inc., pp. 263–275.10.1016/B978-0-12-411584-2.00014-7Google Scholar
Ross, and Senderoff, (1992) Induction of defense enzymes and phenolic content. Ph.D Thesis, Tamil Nadu Agriculture University, Coimbatore, India, pp. 192.Google Scholar
Sadasivam, S and Manickam, A (1996) In: Biochemical Methods. New Age International (P) Limited, New Delhi, Vol. 2, pp. 124–126.Google Scholar
Saleem, M, Haris, WAA and Malik, A (1998) Inheritance of yellow mosaic virus in mungbean (Vigna radiata L. Wilczek). Pakistan Journal of Phytopathology 10: 30–32.Google Scholar
Sandhu, TS, Brar, JS, Sandhu, SS and Verma, MM (1985) Inheritance of resistance to mungbean yellow mosaic virus in greengram. Journal of Research Punjab Agricultural University 22: 607–611.Google Scholar
Shahakar, S, Renuka, and Pater, A (2018) Molecular characterization of virus strain causing yellow mosaic disease (YMD) in mungbean (Vigna radiata L. Wilczek). International Journal of Current Microbiology and Applied Sciences 7: 3727–3744.Google Scholar
Shukla, GP and Pandya, BP (1985) Resistance to yellow mosaic in greengram. SABRAO Journal 17: 165–171.Google Scholar
Sudha, M, Karthikeyan, A, Shoana, VG and Nagarajan, P (2015) Search for Vigna species conferring resistance to mungbean yellow mosaic virus in mungbean. Plant Genetic Resources: Characterization and Utilization 13: 162–167.10.1017/S1479262114000859Google Scholar
Umesha, S (2006) Phenylalanine ammonia lyase activity in tomato seedlings and its relationship to bacterial canker disease resistance. Phytoparasitica 34: 68–71.Google Scholar
Verma, RPS and Singh, DP (1988) Inheritance of resistance to mungbean yellow mosaic virus in greengram. Annual Agriculture Research 9: 98–100.Google Scholar
Vidaysky, F and Czosnek, H (1998) Tomato breeding lines resistant and tolerant to Tomato yellow leaf curl virus issued from Lycopersicon esculentum. Phytopathology 88: 910–914.10.1094/PHYTO.1998.88.9.910Google Scholar
Zeeshan, S, Akhtar, KP, Hameed, A, Sarwar, N, Imran-Ul-Haq, and Khan, SA (2014) Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by cotton leaf curl Burewala virus. Journal of Plant Interactions 9: 702–711.Google Scholar
Zeier, J, Delledonne, M, Mishina, T, Severi, E, Sonoda, M and Lamb, C (2004) Genetic elucidation of nitric oxide signalling in incompatible plat-pathogen interactions. Plant Physiology 136: 2875–2886.Google Scholar
Supplementary material: File

Nagaraj et al. supplementary material

Figure S1

Download Nagaraj et al. supplementary material(File)
File 759 KB
Supplementary material: File

Nagaraj et al. supplementary material

Table S1

Download Nagaraj et al. supplementary material(File)
File 12.5 KB