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High-molecular-weight (HMW) glutenin subunit composition of the Elite-II synthetic hexaploid wheat subset (Triticum turgidum × Aegilops tauschii; 2n = 6x = 42; AABBDD)

Published online by Cambridge University Press:  14 November 2011

Amna Bibi
Department of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Awais Rasheed*
Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Alvina Gul Kazi
National University of Science and Technology (NUST), Islamabad, Pakistan
Tariq Mahmood
Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Saifullah Ajmal
Department of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Iftikhar Ahmed
National Agriculture Research Center (NARC), Islamabad, Pakistan
Abdul Mujeeb-Kazi
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
*Corresponding author. E-mail:


Characterization of high-molecular-weight (HMW) glutenins is an important criterion for identifying genotypes with good bread-making quality. In synthetic hexaploids (SHs), the D-genome encodes several allelic variants of HMW glutenins that require proper identification prior to their utilization for bread wheat (BW) improvement. In this study, SHs with promising agronomic features were characterized for HMW glutenin composition. Seven different allelic variants were observed at the Glu-Dt1 locus, three of which (1Dx1.5+1Dy10, 1Dx1.5+1Dy12.2 and 1Dx2.1+1Dy10) have not been previously reported in existing BW germplasm. The results also showed a variety of D-genome-encoded subunits along with superior glutenin alleles in the B-genome (1Bx7+1By8, 1Bx6+1By8 and 1Bx13+1By16). About 63% of these SHs encoded favourable allelic variants of HMW glutenins, which make them a good choice for improvement in wheat bread making. Glu-Dt1 encoded favourable allelic variants (1Dx5+1Dy10 and 1Dx1.5+1Dy10) that are frequently observed in SHs can be easily incorporated into BW through recombination breeding.

Short Communication
Copyright © NIAB 2011

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