Hostname: page-component-5d59c44645-mrcq8 Total loading time: 0 Render date: 2024-02-28T23:05:40.930Z Has data issue: false hasContentIssue false

High-molecular-weight (HMW) glutenin subunit composition of the Elite-II synthetic hexaploid wheat subset (Triticum turgidum × Aegilops tauschii; 2n = 6x = 42; AABBDD)

Published online by Cambridge University Press:  14 November 2011

Amna Bibi
Affiliation:
Department of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Awais Rasheed*
Affiliation:
Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Alvina Gul Kazi
Affiliation:
National University of Science and Technology (NUST), Islamabad, Pakistan
Tariq Mahmood
Affiliation:
Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Saifullah Ajmal
Affiliation:
Department of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Iftikhar Ahmed
Affiliation:
National Agriculture Research Center (NARC), Islamabad, Pakistan
Abdul Mujeeb-Kazi
Affiliation:
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
*
*Corresponding author. E-mail: awaispbg@gmail.com

Abstract

Characterization of high-molecular-weight (HMW) glutenins is an important criterion for identifying genotypes with good bread-making quality. In synthetic hexaploids (SHs), the D-genome encodes several allelic variants of HMW glutenins that require proper identification prior to their utilization for bread wheat (BW) improvement. In this study, SHs with promising agronomic features were characterized for HMW glutenin composition. Seven different allelic variants were observed at the Glu-Dt1 locus, three of which (1Dx1.5+1Dy10, 1Dx1.5+1Dy12.2 and 1Dx2.1+1Dy10) have not been previously reported in existing BW germplasm. The results also showed a variety of D-genome-encoded subunits along with superior glutenin alleles in the B-genome (1Bx7+1By8, 1Bx6+1By8 and 1Bx13+1By16). About 63% of these SHs encoded favourable allelic variants of HMW glutenins, which make them a good choice for improvement in wheat bread making. Glu-Dt1 encoded favourable allelic variants (1Dx5+1Dy10 and 1Dx1.5+1Dy10) that are frequently observed in SHs can be easily incorporated into BW through recombination breeding.

Type
Short Communication
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Gianibelli, MC, Gupta, RB, Lafiandra, D, Margiotta, B and MacRitchie, F (2001) Polymorphism of high M r glutenins in Triticum tauschii: characterisation by chromatography and electrophoretic methods. Journal of Cereal Science 33: 3952.CrossRefGoogle Scholar
Hsam, SLK, Kieffer, R and Zeller, FJ (2001) Significance of Aegilops tauschii glutenin genes on bread-making properties of wheat. Cereal Chemistry 78: 521525.CrossRefGoogle Scholar
Lagudah, ES, Flood, RG and Halloran, GM (1987) Variation in high molecular weight glutenin subunits in landraces of hexaploids wheat from Afghanistan. Euphytica 36: 39.CrossRefGoogle Scholar
Margiotta, B, Colaprico, G, D'Ovidio, R and Lafiandra, D (1993) Characterization of high M r subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoretic separations and restriction fragment length polymorphism (RFLP) analyses of their encoding genes. Journal of Cereal Science 17: 221236.CrossRefGoogle Scholar
Mujeeb-Kazi, A, Rosas, V and Roldan, S (1996) Conservation of the genetic variation of Triticum tauschii (Coss.) Schmalh. (Aegilops squarrosa auct. Non L.) in synthetic hexaploid wheats (T. turgidum L. s. lat. × T. tauschii; 2n = 6x = 42, AABBDD) and its potential utilization for wheat improvement. Genetic Resources and Crop Evolution 43: 129134.CrossRefGoogle Scholar
Payne, PI and Lawrence, GJ (1983) Catalogue or alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for the high-molecular weight subunit of glutenin whose in hexaploid wheat. Cereal Research Communications 11: 2935.Google Scholar
Peña, RJ, Zarco-Hernandez, J and Mujeeb-Kazi, A (1995) Glutenin subunit compositions and bread making quality characteristics of synthetic hexaploid wheats derived from Triticum turgidum × Triticum tauschii (coss.) Schmal Crosses. Journal of Cereal Science 21: 1523.CrossRefGoogle Scholar
Rehman, A, Evans, N, Gianibelli, MC and Rose, RJ (2008) Allelic variation in high and low molecular weight glutenins at the Glu-D t locus of Aegilops tauschii as a potential source for improving bread wheat quality. Australian Journal of Agricultural Research 59: 399405.CrossRefGoogle Scholar
Saffdar, H, Ashfaq, M, Hameed, S, Ul-Haq, I and Mujeeb-Kazi, A (2009) Molecular analysis of genetic diversity in Elite-II synthetic hexaploid wheat screened against barley yellow dwarf virus. African Journal of Biotechnology 14: 32443250.Google Scholar
Tang, Y, Yang, W, Tian, J, Li, J and Chen, F (2008) Effect of HMW-GS 6+8 and 1.5+10 from synthetic hexaploid wheat on wheat quality trait. Agriculture Sciences in China 7: 11611171.CrossRefGoogle Scholar
Tang, Y, Yang, W, Wu, Y, Li, C, Li, J, Zou, Y, Chen, F and Mares, D (2010) Effect of high molecular weight glutenin allele, Glu-B1d, from synthetic hexaploid wheat on wheat quality parameters and dry, white Chinese noodle-making quality. Australian Journal of Agricultural Research 61: 310320.Google Scholar
Tariq-Khan, M and Ul-Haq, I (2011) Elite-II synthetic hexaploids wheats as a potential source of resistance against yellow rust. Archives of Phytopathology and Plant Protection 44: 11651170.CrossRefGoogle Scholar
William, MDHM, Peña, RJ and Mujeeb-Kazi, A (1993) Seed protein and isozyme variations in Triticum tauschii (Aegilops squarrosa). Theoretical and Applied Genetics 87: 257263.CrossRefGoogle ScholarPubMed
Xu, SS, Khan, K, Klindworth, DL and Nygard, G (2010) Evaluation and characterization of high-molecular weight 1D glutenin subunits from Aegilops tauschii in synthetic hexaploid wheats. Journal of Cereal Science 52: 333336.CrossRefGoogle Scholar