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Genotypic identification and diversity evaluation of a sweet potato (Ipomoea batatas (L). Lam) collection using microsatellites

Published online by Cambridge University Press:  10 October 2008

C. M. Arizio ,
N. Hompanera ,
E. Y. Suarez  and
M. M. Manifesto
C. M. Arizio
Affiliation:
Instituto de Recursos Biológicos, CIRN-INTA, Las Cabañas y Los Reseros s/n. 1712 Castelar, Buenos Aires, Argentina
N. Hompanera
Affiliation:
Instituto de Recursos Biológicos, CIRN-INTA, Las Cabañas y Los Reseros s/n. 1712 Castelar, Buenos Aires, Argentina
E. Y. Suarez
Affiliation:
Instituto de Recursos Biológicos, CIRN-INTA, Las Cabañas y Los Reseros s/n. 1712 Castelar, Buenos Aires, Argentina
M. M. Manifesto*
Affiliation:
Instituto de Recursos Biológicos, CIRN-INTA, Las Cabañas y Los Reseros s/n. 1712 Castelar, Buenos Aires, Argentina
*
*Corresponding author. E-mail: mmanifesto@cnia.inta.gov.ar
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Abstract

Characterization and genotype identification are essential for rational conservation, management and genetic diversity evaluation. In this work, simple sequence repeat (SSR) markers were used to identify 57 accessions of sweet potato kept at the ‘in vitro’ Gene Bank of INTA, Argentina. This collection includes primitive materials from primary zones of dispersion, commercial varieties, breeding clones and foreign materials from different parts of the world (Africa, Asia and USA). Average similarity between the materials evaluated was 0.367. Grouping analysis revealed six clusters and a strong association with skin colour of storage roots. The set of selected SSR markers allowed us to verify identity, detect duplicates and estimate the genetic similarity of the materials analysed. This characterization complements morphological data and provides tools to estimate the variation of diversity between local and foreign germplasm groups.

Keywords

diversitygenotype identificationgermplasmIpomoea batatasmicrosatellites
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 2 , August 2009 , pp. 135 - 138
Copyright
Copyright © NIAB 2008

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References

Bowman, KO, Hutcheson, K, Odum, EP and Shenton, LR (1971) Comments on the distribution of indices of diversity. Statistical Ecology 3: 315–359.Google Scholar
Buteler, MI, Jarret, RL and LaBonte, DR (1999) Sequence characterization of microsatellites in diploid and polyploid Ipomoea. Theoretical and Applied Genetics 99: 123–132.CrossRefGoogle Scholar
Dhillon, NPS and Ishiki, K (1999) Genomic variation and genetic relationships in Ipomoea spp. Plant Breeding 118: 161–165.CrossRefGoogle Scholar
Excoffier, L, Smouse, P and Quattro, J (1992) Analysis of molecular human mitochondrial DNA restriction data. Genetics 131: 479–491.CrossRefGoogle ScholarPubMed
He, GH, Prakash, CS and Jarret, RL (1995) Analysis of genetic diversity in sweet-potato (Ipomoea batatas) germplasm collection using DNA amplification fingerprinting. Genome 38: 938–945.CrossRefGoogle ScholarPubMed
Jarret, RL and Bowen, N (1994) Simple sequence repeats (SSRs) for sweet potato germoplasm characterization. Plant Genetics Resources Newsletter 100: 9–11.Google Scholar
Tseng, YT, Lo, HF and Hwang, SY (2002) Genotyping and assessment of genetic relationships in elite polycross breeding cultivars of sweetpotato in Taiwan based on SAMPL polymorphisms. Botanical Bulletin Academia Sinica 43: 99–105.Google Scholar
Wang, J, He, G, Prakash, CS and Lu, S (1998) Analysis of genetic diversity in Chinese sweetpotato (Ipomoea batatas (L Lam.) germplasm using DNA amplification fingerprinting. Plant Genetic Resources Newsletter 113: 13–16.Google ScholarPubMed
Zhang, DP, Ghislain, M, Huamán, Z, Golmirzaie, A and Hijmans, R (1998) RAPD variation in sweetpotato (Ipomoea batatas (L.) Lam) cultivars from South America and Papua New Guinea. Genetic Resources and Crop Evolution 45: 271–277.CrossRefGoogle Scholar
Zhang, DP, Carbajulca, D, Ojeda, L, Rossel, G, Mill, S, Herrera, C and Ghislain, M (2000) Microsatellite analysis of genetic diversity in sweetpotato varieties from Latin America. CIP Program Report: 1999–2000.Google Scholar