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Genetic diversity and phylogeny analysis of 3-hydroxy 3-methylglutaryl-CoA reductase gene (SmHMGR) in Danshen (Salvia miltiorrhiza Bunge)

Published online by Cambridge University Press:  18 May 2022

Ruihua Ren
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Fang Liao
Affiliation:
Animal, Plant and Foodstuff Inspection Center, Tianjin Customs, Tianjin 300461, China
Deying Kong
Affiliation:
Technology Center, Chongqing Customs, Chongqing, 401147, China
Yanyan Yin
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Wei Liu
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Shaona Teng
Affiliation:
Technology Center, Chongqing Customs, Chongqing, 401147, China
Jie Feng
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Guanrong Li*
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
*
Author for correspondence: Guanrong Li, E-mail: grli1963@126.com

Abstract

HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase, is a major rate-limiting enzyme in mevalonate (MVA) pathway for isoprenoids and subsequent tanshinone biosynthesis in the Chinese traditional bulk herbal medicine Danshen, Salvia miltiorrhiza, mainly for cardiovascular disorders. In this paper, the genomic SmHMGR genes of 38 cultivated populations of S. miltiorrhiza collected in China were for the first time sequenced to reveal the genetic diversity and phylogeny. The SmHMGR gene was shown to be intron-free, 1650~1659 bp in complete CDS with the majority being 1656 bp, and two unique populations (W-FJLY-V-1 and W-SCHY-W-1) being 1659 and 1650 bp respectively. A total of 103 SNP variation sites were detected with a variation rate of 6.22%, most of which occurred in S. miltiorrhiza f. alba population W-SCHY-W-1; a total of 25 amino acid variation sites were found, of which 19 was in W-SCHY-W-1. The same four populations, W-SCHY-W-1, V-HBAG-V-1, V-JLCC-V-1 and S-NM-V-1 could be discriminated from the remaining 34 by both the SNP fingerprints and the deduced amino acid variation sites. Other or composite DNA markers are needed for better identification. The SmHMGR gene of white flower S. miltiorrhiza f. alba population W-SCHY-W-1 is especially rich in variations and worthy of further studies. Phylogenetic trees based on both the gene and the deduced amino acid sequences showed a very similar two-clade topological structure. This research enriched the content and the genetic means for the molecular identification, genetic diversity and phylogenetic studies of the cultivated S. miltiorrhiza populations, and laid a solid foundation for further related and in-depth investigations.

Type
Research Article
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

Equal contributing authors.

References

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Genetic diversity and phylogeny analysis of 3-hydroxy 3-methylglutaryl-CoA reductase gene (SmHMGR) in Danshen (Salvia miltiorrhiza Bunge)
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