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Evaluation of iron and zinc in grain and grain fractions of hexaploid wheat and its related species for possible utilization in wheat biofortification

Published online by Cambridge University Press:  16 April 2015

Upendra Kumar
Affiliation:
Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Udham Singh Nagar, Uttarakhand, India
Priyanka Mathpal
Affiliation:
Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Udham Singh Nagar, Uttarakhand, India
Sachin Malik
Affiliation:
Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Udham Singh Nagar, Uttarakhand, India
Naveen Kumar
Affiliation:
Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Udham Singh Nagar, Uttarakhand, India
Satish Kumar
Affiliation:
Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, Uttarakhand, India
Vishal Chugh
Affiliation:
Akal School of Biotechnology, Eternal University, Baru Sahib via Rajgarh, Distt. Sirmour-173101, Himachal Pradesh, India
Imran Sheikh
Affiliation:
Akal School of Biotechnology, Eternal University, Baru Sahib via Rajgarh, Distt. Sirmour-173101, Himachal Pradesh, India
Prachi Sharma
Affiliation:
Akal School of Biotechnology, Eternal University, Baru Sahib via Rajgarh, Distt. Sirmour-173101, Himachal Pradesh, India
Tejveer Singh
Affiliation:
Division of Crop Improvement, Indian Grassland and Fodder Research Institute, Jhansi-284003, Uttar Pradesh, India
H. S. Dhaliwal
Affiliation:
Akal School of Biotechnology, Eternal University, Baru Sahib via Rajgarh, Distt. Sirmour-173101, Himachal Pradesh, India
Sundip Kumar*
Affiliation:
Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Udham Singh Nagar, Uttarakhand, India
*
*Corresponding author. E-mail: malik.sundip@gmail.com

Abstract

Iron (Fe) and zinc (Zn) contents in hexaploid wheat are very low and are further reduced because of the removal of micronutrient-rich bran of wheat grains during milling and processing. Therefore, hexaploid wheat, its wild species and wheat–Aegilops kotschyi substitution lines were evaluated to identify the genome(s) carrying gene(s) for high Fe and Zn concentrations in bran and endosperm fractions of grains. It is reflected from the results that Triticum monococcum (acc. W463) may serve as a promising donor for biofortification of Fe, and Aegilops speltoides (acc. 3804) may serve as a promising donor for biofortification of Zn in the endosperm of cultivated wheat. Further, among the three wheat–Ae. kotschyi substitution lines, the higher concentration of Fe and Zn in endosperm fraction was observed in BC2F4 63-2-13-1. The work on precise transfer of useful gene(s) from 7Uk chromosome of this line is in progress to reduce linkage drag.

Type
Research Article
Copyright
Copyright © NIAB 2015 

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