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Unique genetic structure of the human tapeworm Dibothriocephalus latus from the Alpine lakes region – a successful adaptation?

Published online by Cambridge University Press:  16 May 2022

Alžbeta Radačovská Open the ORCID record for Alžbeta Radačovská [Opens in a new window] ,
Eva Čisovská Bazsalovicsová Open the ORCID record for Eva Čisovská Bazsalovicsová [Opens in a new window] ,
Katarína Šoltys Open the ORCID record for Katarína Šoltys [Opens in a new window] ,
Jan Štefka Open the ORCID record for Jan Štefka [Opens in a new window] ,
Gabriel Minárik ,
Andrea Gustinelli ,
Julia K. Chugunova Open the ORCID record for Julia K. Chugunova [Opens in a new window]  and
Ivica Králová-Hromadová Open the ORCID record for Ivica Králová-Hromadová [Opens in a new window]
Alžbeta Radačovská
Affiliation:
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia
Eva Čisovská Bazsalovicsová
Affiliation:
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia
Katarína Šoltys
Affiliation:
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 84215 Bratislava, Slovakia
Jan Štefka
Affiliation:
Biology Centre CAS, Institute of Parasitology, Branišovská 31, 37005 České Budějovice, Czech Republic Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech Republic
Gabriel Minárik
Affiliation:
Medirex, a.s., Galvaniho 17/C, P.O. Box 143, 82016 Bratislava, Slovakia
Andrea Gustinelli
Affiliation:
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, BO, Italy
Julia K. Chugunova
Affiliation:
Krasnoyarsk Branch of the Russian Federal Research Institute of Fisheries and Oceanography ‘VNIRO’, Parizhskoi Kommuny, 33, 660097 Krasnoyarsk, Russia
Ivica Králová-Hromadová*
Affiliation:
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia
*
Author for correspondence: Ivica Králová-Hromadová, E-mail: hromadova@saske.sk
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Abstract

Dibothriocephalus latus is the most frequent causative agent of fish-borne zoonosis (diphyllobothriosis) in Europe, where it is currently circulating mainly in the Alpine lakes region (ALR) and Russia. Three mitochondrial genes (cox1, cob and nad3) and 6 microsatellite loci were analysed to determine how is the recently detected triploidy/parthenogenesis in tapeworms from ALR displayed at the DNA level. A geographically distant population from the Krasnoyarsk Reservoir in Russia (RU-KR) was analysed as a comparative population. One or 2 alleles of each microsatellite locus was detected in plerocercoids from RU-KR, corresponding to the microsatellite pattern of a diploid organism. In contrast, 1–3 alleles were observed in tapeworms from ALR, in accordance with their triploidy. The high diversity of mitochondrial haplotypes in D. latus from RU-KR implied an original and relatively stable population, but the identical structure of mitochondrial genes of tapeworms from ALR was probably a consequence of a bottleneck typical of introduced populations. These results indicated that the diploid/sexually reproducing population from RU-KR was ancestral, located within the centre of the distribution of the species, and the triploid/parthenogenetically reproducing subalpine population was at the margin of the distribution. The current study revealed the allelic structure of the microsatellite loci in the triploid tapeworm for the first time.

Keywords

Asexual populationDiphyllobothriosisfish-borne zoonosishaplotypesmicrosatellitesmitochondrial DNAparthenogenesistriploid tapeworms
Type
Research Article
Information
Parasitology , Volume 149 , Issue 8 , July 2022 , pp. 1106 - 1118
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Copyright © The Author(s), 2022. Published by Cambridge University Press

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Footnotes

*

Equal contribution of both authors.

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