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Trichinella strain, pig race and other parasitic infections as factors in the reliability of ELISA for the detection of swine trichinellosis

Published online by Cambridge University Press:  06 April 2009

F. Serrano
Affiliation:
Parasitology and Parasitic Diseases Section, Department of Animal Health and Medicine, Faculty of Veterinary Sciences, University of Extremadura, 10071 Cáceres, Spain
E. Pérez
Affiliation:
Parasitology and Parasitic Diseases Section, Department of Animal Health and Medicine, Faculty of Veterinary Sciences, University of Extremadura, 10071 Cáceres, Spain
D. Reina
Affiliation:
Parasitology and Parasitic Diseases Section, Department of Animal Health and Medicine, Faculty of Veterinary Sciences, University of Extremadura, 10071 Cáceres, Spain
I. Navarrete
Affiliation:
Parasitology and Parasitic Diseases Section, Department of Animal Health and Medicine, Faculty of Veterinary Sciences, University of Extremadura, 10071 Cáceres, Spain

Extract

An enzyme-linked immunosorbent assay (ELISA) using crude worm extracts (CWE) and mixtures of these as antigens of five Spanish isolates (P, C, B1, B2 and W) was developed for detecting homologous and heterologous experimental infections with these isolates between – 14 and 82 days post-infection (p.i.) in white and Iberian pigs. A total of 243 pigs (Ilberian or cross-bred with this race) with numerous parasitic infections were also screened for the presence of antibodies to a mixture of CWE of C, B1 and B2 isolate. The test showed a specificity of 93·1–98·9% depending on the cut-off values and a maximum sensitivity of 92·8–100% between days 34 and 82 p.i. A low grade of infectivity was shown in the T3 isolates compared to the T1 isolates (P, C, B1 and B2) but high cross-reactions were observed between all the isolates with minor differences between P and W isolates. The highest antibody response was found in P infections and the lowest in pigs infected with the W isolate. A clear association between the presence of several parasitic infections and false positive reactions was not found, but an important relation was shown between high background levels and the Iberian race in experimentally and conventionally raised pigs

Type
Research Article
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Armas Serra, C., Gómez Barrio, A., Bolas Fernández, F. & Martínez Fernández, A. R. (1989). Multifactorial analysis of morpho-biological parameters in Spanish Trichinella isolates. In Trichinellosis (ed. Tanner, C. E., Martinez Fernández, A. R. & Bolas-Fernández, F.), pp. 102–6. Madrid: C.S.I.C. Press.Google Scholar
Arriaga, C., Muñiz, E., Morilla, A. & Ortega-Pierres, M. G. (1989). Evaluation of purified surface stichosomal components of Trichinella spiralis muscle larvae for diagnosis of swine trichinellosis. In Trichinellosis (ed. Tanner, C. E., Martínez Fernández, A. R. & Bolas-Fernández, F.), pp. 182–7. Madrid: C.S.I.C. Press.Google Scholar
Clinard, E. H. (1979). Identification and distribution of swine serum immunoglobulins that react with Trichinella spiralis antigens and may interfere with the enzyme-labeled antibody test for trichinosis. American Journal of Veterinary Research 40, 1558–63.Google Scholar
Čuperlović, K., Movsesijan, M., Ivanoska, D., Sofronić, I. & Jovanović, B. (1987). Detection of antibodies to Trichinella spiralis in farm pigs. Veterinarski Archiv 57, 133–41.Google Scholar
Erber, M. (1977). Möglichkeiten des Nachweises und der Differenzierung von zwei Sarcocystis Arten des Schweines. Berliner und Münchener Tierärztliche Wochenschrift 90, 480–2.Google Scholar
Gamble, H. R., Anderson, W. R., Graham, C. E. & Murreli, K. D. (1983). Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory–secretory antigen. Veterinary Parasitology 13, 349–61.CrossRefGoogle ScholarPubMed
Isenstein, R. S., Webert, D. W., Leighty, J. C., Zimmermann, W. J., Singh, P., Oliver, D. G. & Jang, L. (1989). Development of an industrial immunoassay for trichinellosis. In Trichinellosis (ed. Tanner, C. E., Martínez Fernández, A. R. & Bolas-Fernández, F.), pp. 262–7. Madrid: C.S.I.C. Press.Google Scholar
Martínez Fernández, A. R., Armas Serra, A., Gómez Barrio, A. & Bolas Fernández, F. (1989). Single-pairs cross-hybridization test among Spanish Trichinella isolations. In Trichinellosis (ed. Tanner, C. E., Martínez Fernández, A. R. & Bolas-Fernández, F.), pp. 96101. Madrid: C.S.I.C. Press.Google Scholar
Murreli, K. D., Anderson, W. R., Schad, G. A., Hanbury, R. D., Kazacos, K. R., Gamble, H. R. & Brown, J. (1986). Field evaluation of the enzyme-linked immunosorbent assay for swine trichinosis: efficacy of the excretory–secretory antigen. American Journal of Veterinary Research 47, 1046–9.Google Scholar
Pozio, E., La Rosa, G., Rossi, P. & Murrell, K. D. (1989). New taxonomic contribution to the genus Trichinella (Owen, 1835). I. Biochemical identification of seven clusters by gene-enzyme systems. In Trichinellosis (ed. Tanner, C. E., Martínez Fernández, A. R. & Bolas-Fernández, F.), pp. 7682. Madrid: C.S.I.C. Press.Google Scholar
Ruitenberg, E. J., Steerenberg, P. A., Brosi, B. J. M. & Buys, J. (1975). ELISA (enzyme-linked immunosorbent assay) as a preventive and representative control method for the detection of Trichinella spiralis infections in slaughter pigs. Wiadomosci Parazytologiczne 21, 747–51.Google Scholar
Ruitenberg, E. J., Van Knapen, F. & Vermeulen, C. J. (1978). Enzyme-linked immunosorbent assay (ELISA) in Trichinella spiralis infections in pigs. In Trichinellosis (ed. Kim, C. & Paulowski, Z.), pp. 487–9. Hanover, USA: University of New England Press.Google Scholar
Seawright, G. L., Despommier, D. D., Zimmermann, W. & Isenstein, R. S. (1983). Enzyme immunoassay for swine trichinosis using antigens purified by immunoaffinity chromatography. American Journal of Tropical Medicine and Hygiene 32, 1275–84.CrossRefGoogle ScholarPubMed
Smith, H. J. & Snowdon, K. E. (1987). Detection of Trichinella spiralis nativa antibodies in porcine sera by ELISA using T. spiralis spiralis excretory–secretory antigen. Canadian Journal of Veterinary Research 51, 413–14.Google ScholarPubMed
Smith, H. J. & Snowdon, K. E. (1989). Comparative assessment of a double antibody enzyme immunoassay test kit and a triple antibody enzyme immunoassay for the diagnosis of Trichinella spiralis spiralis and Trichinella spiralis nativa infections in swine. Canadian Journal of Veterinary Research 53, 497–9.Google Scholar
Su, X. & Prestwood, A. K. (1991). A dot-ELISA mimicry western blot test for the detection of swine trichinellosis. Journal of Parasitology 77, 7682.Google Scholar
Van Knapen, F. & Franchimont, J. H. (1984). Further studies with sera from Trichinella spiralis free pigs from 10 European countries and Canada to determine the detection levels for the enzyme-linked immunosorbent assay (ELISA). Report on behalf of members of the EEC working group on trichinellosis, January 1984.Google Scholar
Van Knapen, F., Franchimont, J. H. & Ruitenberg, E. J. (1981). The reliability of the enzyme-linked immunosorbent assay (ELISA) for the detection of swine trichinellosis. In Trichinellosis (ed. Kim, C. W., Ruitenberg, E. J. & Teppema, J. S.), pp. 399404. Chertsey: Reedbooks. Reported on behalf of the members of the EEC working group on trichinellosis (1981).Google Scholar
Van Knapen, F., Franchimont, J. H., Ruitenberg, E. J., André, P., Baldelli, B., Gibson, T. E., Henriksen, S. A., Köhler, G., Roneus, O., Skovgaard, N., Soule, C., Strickland, K. L., Taylor, S. M., Thomsem, D. U. & Wolff, F. (1984 a). Comparison of three methods for detection of prolonged experimental trichinellosis in pigs. Veterinary Parasitology 16, 167–71.CrossRefGoogle ScholarPubMed
Van Knapen, F., Franchimont, J. H., Ruitenberg, E. J., Baldelli, B., Bradley, J., Gibson, T. E., Gottal, C., Henriksen, S. A., Köhler, G., Skovgaard, N., Soule, C. & Taylor, S. M. (1980). Comparison of the enzyme- linked immunosorbent assay (ELISA) with three other methods for the detection of Trichinella spiralis infection in pigs. Veterinary Parasitology 7, 109–21.Google Scholar
Van Knapen, F., Franchimont, J. H., Skovgaard, N., Guildal, J. & Henriksen, S. A. (1984 b). Husbandry, parasitic and other diseases as factors in the reliability of the enzyme-linked immunosorbent assay (ELISA) for detection of trichinellosis in pigs. Veterinary Parasitology 16, 1722.CrossRefGoogle ScholarPubMed
Van Knapen, F. & Ruitenberg, E. J. (1979). Report 1977–1978 concerning Trichinella spiralis studies in The Netherlands. Veterinary Quarterly 1, 166–7.CrossRefGoogle ScholarPubMed