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A study on antigen recognition by onchocerciasis patients with different clinical forms of disease

Published online by Cambridge University Press:  06 April 2009

R. Lucius ,
D. W. Büttner ,
Christa Kirsten  and
H. J. Diesfeld
R. Lucius
Affiliation:
Institut für Tropenhygiene und öffentliches Gesundheitswesen derUniversität Heidelberg, Im Neuenheimer Feld 324, D-69 Heidelberg, FRG
D. W. Büttner
Affiliation:
Bernhard-Nocht-Institutfür Schiffs- und Tropenkrankheiten, Bernhard-Nocht-Str. 74, D-2000 Hamburg 4, FRG
Christa Kirsten
Affiliation:
Institut für Tropenhygiene und öffentliches Gesundheitswesen derUniversität Heidelberg, Im Neuenheimer Feld 324, D-69 Heidelberg, FRG
H. J. Diesfeld
Affiliation:
Institut für Tropenhygiene und öffentliches Gesundheitswesen derUniversität Heidelberg, Im Neuenheimer Feld 324, D-69 Heidelberg, FRG
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Summary

Sera from 40 onchocerciasis patients from the Yemen Arab Republic with either mild localized forms of onchocerciasis, intermediate or severe localized forms of the disease or generalized forms of infection were studies with rspect to their IgG and IgM response against Onchocerca volvulus antigens. Immunoblotting, performed with SDS–PAGE-separated proteins of female O. volvulus and quantified by densitometric scanning, revealed IgG and IgM antibodies against worm components in sera of all patients. Persons with intermediate or severe localized forms of onchocerciasis had a stronger IgG response against more proteins than individuals of the other group. However, some antigens (Mr 21, 23, 30, 33 kDa) induced comparable quantities of IgG in all groups. The IgM response of patients with mild localized forms of onchocerciasis was more intensive and directed against more antigensthan in the other groups. No antigens were detected that were recognized only by individuals with low levels of microfilaridermia. In all groups, varying concentrations of antibodies against cuticle, muscle/hypodermis layer and/or uterus of female O. volvulus were detected by the indirect immunofluorescence test using frozen worm sections as antigen. The highest mean antibody titres were found in patients with intermediate and severe localized forms of disease.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 3 , June 1986 , pp. 569 - 580
Copyright
Copyright © Cambridge University Press 1986

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References

REFERENCES

Anderson, J., Fuglsang, H. & AlZubaidy, A. (1973). Onchocerciasis in Yemen with special reference to sowda. Transactions of the Royal Society of Tropical Medicine and Hygiene 67, 30–1.CrossRefGoogle ScholarPubMed
Bartlett, A., Turk, J., Ngu, J., Mackenzie, C. D., Fuglsang, H. & Anderson, J. (1978). Variation in delayed hypersensitivity in onchocerciasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 372–7.Google Scholar
Boto, W. O., Powers, K. G. & Levy, D. A. (1984). Antigens of Dirofilaria immitis which are immunogenic in the canine host. Detection by immuno-staining of protein blots with the antibodies of occult dogs. Journal of Immunology 133, 975–80.CrossRefGoogle ScholarPubMed
Burnette, W. N. (1981). ‘Western Blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate – polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112, 195–203.CrossRefGoogle ScholarPubMed
Büttner, D. W. V., Laer, G., Mannweiler, E. & Büttner, M. (1982). Clinical, parasitological and serological studies on onchocerciasis in the Yemen Arab republic. Tropenmedizin und Parasitologie 33, 201–12.Google ScholarPubMed
Connor, D. H., Gibson, D. W., Neafie, R. C., Merighi, B. & Buck, A. A. (1983). Sowda–onchocerciasis in North Yemen: a clinicopathological study of 18 patients. American Journal of Tropical Medicine and Hygiene 32, 123–37.CrossRefGoogle ScholarPubMed
Diesfeld, H. J., Kirsten, C. & Stappert, U. (1981). Vergleichende Immunofiuoreszenz-histologische Untersuchungen an 7 Nematoden-Spezies in bezug auf ihre Antigengemeinschaften zur Differenzierung von Nematodeninfektionen. Tropenmedizin und Parasitologie 32, 253–8.Google Scholar
Felgner, P. (1978). Stepless antibody determination with stick-ELISA technique. Results expressed as multiple of normal activity. Zentralblatt für Bakteriologie und Hygiene, I. Abteilung Originale A242, 100–5.Google Scholar
Fletcher, C. & Birch, D. W. (1985). A search for Brugia pahangi antigens relevant to host-protective immunity in infected cats. Parasitology 91, 410.Google Scholar
Gasparini, G. (1962). ‘Sowda’, nuova malattia o forma inedita di onchocercosi? Archivio Italiano di Scienze Mediche Tropicali e di Parassitologia 43, 635–46.Google Scholar
Gasparini, G. (1964). Problems of onchocerciasis in new suspected areas: Sowda and onchocerciasis. Archivio Italiano di Scienze Mediche Tropicale e di Parassitologia 45, 243–60.Google ScholarPubMed
Greene, B. M. (1980). Cell mediated killing of microfilariae of Onchocerca volvulus. Clinical Research 38, 370.Google Scholar
Hawkes, R., Niday, E. & Gordon, J. (1982). Dot immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119, 142–7.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Lammie, P. J. & Katz, S. P. (1983). Immunoregulation in experimental filariasis: II. Responses to parasite and to nonparasite antigens in jirds infected with Brugia pahangi. Journal of Immunology 130, 1386–9.Google Scholar
Lienert, G. A. (1973). Verteilungsfreie Methoden in der Biostatistik. Meisenheim am Glan: Verlag Anton Hain.Google Scholar
Lucius, R., Rauterberg, E. W. & Diesfeld, H. J. (1983). Identification of immunogenic proteins of Dipetalonema viteae (Filarioidea) by the ‘Western Blotting’ technique. Tropenmedizin und Parasitologie 34, 133–6.Google ScholarPubMed
Lucius, R., Well, B. & Diesfeld, H. J. (1984). The antibody response to Onchocerca volvulus antigens shows individual differences. Zentralblatt für Bakteriologie und Hygiene A258, 419.Google Scholar
Mackenzie, C. D. (1980). Eosinophil leucocytes in filarial infections. Transactions of the Royal Society of Tropical Medicine and Hygiene 74, 51–8.Google Scholar
Ottesen, E. A., Weller, P. F. & Heck, L. (1977). Specific cellular immune unresponsiveness in human filariasis. Immunology 33, 413–21.Google Scholar
Phillip, M., Gomez-Priego, A., Parkhouse, R. M. E., Davies, M. W., Clark, N. W. T., Ogilvie, B. M. & Beltran-Hernandez, F. (1984). Identification of an antigen of Onchocerca volvulus of possible diagnostic use. Parasitology 89, 295–309.CrossRefGoogle Scholar
Piessens, W. F. & Mackenzie, C. D. (1982). Immunology of lymphatic filariasis and onchocerciasis. In Immunology of Parasitic Infections, 2nd Edn (ed. Cohen, S and Warren, K. S.), Oxford: Blackwell Scientific Publications.Google Scholar
Piessens, W. F., Partono, F., Hoffmann, S. L., Ratiwayanto, S., Piessens, P. W., Palmieri, J. R., Koiman, I., Dennis, D. T. & Carney, W. P. (1982). Antigen-specificsuppressorT-lymphocytes in human lymphatic filariasis. New England Journal of Medicine 307, 144–8.CrossRefGoogle ScholarPubMed
Piessens, W. F., Ratiwayanto, S., Tuti, S., Palmieri, J. H., Piessens, P. W., Koiman, I. & Dennis, D. T. (1980). Antigen-specific suppressor cells and suppressor factors in human filariasis with Brugia malayi. New England Journal of Medicine 302, 833–7.Google Scholar
Prod'Hon, J., Lucius, R., Kern, A., Hebrard, G. & Diesfeld, H. J. (1985). Studies on the protein and antigen composition of individual female Onchocerca volvulus after collagenase digestion. Tropical Medicine and Parasitology 36, 238–40.Google Scholar
Schulz-Key, H., Albiez, E. J. & Büttner, D. W. (1977). Isolation of living adult Onchocerca volvulus from nodules. Tropenmedizin und Parasitologie 28, 428–30.Google Scholar
Tamashiro, W. K., Powers, K. G., Levy, D. A. & Scott, A. L. (1985). Quantitative and qualitative changes in the humoral response of dogs through the course of infection with Dirofilaria immitis. American Journal of Tropical Medicine and Hygiene 34, 292–301.Google Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle ScholarPubMed
Weiss, N., Gualzata, M., Wyss, T. & Betschart, B. (1982). Detection of IgE-binding Onchocerca volvulus antigens after electrophoretic transfer and immuno-enzyme reaction. Acta Tropica 39, 373–7.Google ScholarPubMed
Zacharius, R. M., Zell, T. E., Morrison, J. H. & Woodlock, J. J. (1969). Glycoprotein staining following electrophoresis on acrylamide gels. Analytical Biochemistry 30, 148–51.CrossRefGoogle ScholarPubMed