Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-22T13:49:46.486Z Has data issue: false hasContentIssue false
  • English
  • Français

Schistosoma mansoni: analysis of the humoral and cellular basis of resistance in guinea-pigs vaccinated with radiation-attenuated cercariae

Published online by Cambridge University Press:  06 April 2009

D. J. McLaren ,
V. S. Delgado ,
J. R. Gordon  and
M. V. Rogers
D. J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
V. S. Delgado
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
J. R. Gordon
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
M. V. Rogers
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Summary

This study addresses the humoral and cellular basis of specific acquired immunity in the guinea-pig irradiated vaccine model of schistosomiasis mansoni. Rodents vaccinated with 500 gamma-irradiated cercariae and then splenectomized 4·5 weeks later showed a 33% reduction in resistance to challenge as compared to vaccinated animals or vaccinated/sham splenectomized controls. Serum harvested from once vaccinated individuals conferred modest levels of resistance upon naive recipients in some experiments, but transfer was not achieved consistently. Serum from vaccinated and thrice boosted rodents (Vbbb) routinely transferred around 45% immunity, however, provided it was given in 4 ml aliquots on day 9 post-challenge; Vbbb serum thus transferred 50% of donor immunity. Interestingly, multiple doses of this protective serum given on and either side of day 9 did not enhance the protection achieved with a single 4 ml aliquot. Neither peripheral lymph node cells nor splenocytes from the polyvaccinated serum donors were able to transfer resistance to recipient guinea-pigs and they failed to augment the protection achieved with Vbbb serum. Food-pad testing revealed no correlation between delayed hypersensitivity responses and immunity to challenge in vaccinated guinea-pigs. Although polyvaccine guinea-pig serum successfully protected homologous recipients, it failed to protect mice when administered either at the time of challenge (the optimal schedule for transfer of polyvaccine mouse serum), or around day 9 (the optimal schedule for guinea-pigs). Similarly, guinea-pigs could not be protected with polyvaccine rat serum that conferred 75% resistance upon naive recipient rats.

Keywords

Schistosoma mansonivaccine immunitypassive transferguinea-pigssplenectomyhypersensitivity reactions
Type
Research Article
Information
Parasitology , Volume 100 , Issue 1 , February 1990 , pp. 35 - 44
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aitken, R., Coulson, S., Dixon, B. & Wilson, A. R. (1987). Radiation-resistant acquired immunity of vaccinated mice to Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 37, 570–7.CrossRefGoogle ScholarPubMed
Delgado, V. S. & McLaren, D. J. (1989). Evidence for enhancement of IgG1 subclass expression in mice polyvaccinated with radiation-attenuated cercariae of Schistosoma mansoni and role of this isotype in serum transferred immunity. Parasite Immunology (in the Press).Google Scholar
Ford, M. J., Bickle, Q. D., Taylor, M. J. & Andrews, B. J. (1984). Passive transfer of resistance and the site of immune dependent elimination of the challenge infection in rats vaccinated with highly irradiated cercariae of Schistosoma mansoni. Parasitology 89, 461–82.CrossRefGoogle ScholarPubMed
Gordon, J. R. & McLaren, D. J. (1987). Immunity to schistosomiasis mansoni in guinea-pigs vaccinated with radiation-attenuated cercariae: I. Humoral responses against skin-stage schistosomula. Clinical and Experimental Immunology 67, 252–61.Google ScholarPubMed
Hudson, L. & Hay, F. C. (1980). Practical Immunology, 2nd edn. Oxford: Blackwell Scientific Publications.Google Scholar
James, S. L. (1986). Induction of protective immunity against Schistosoma mansoni by a non-living vaccine. III. Correlation of resistance with induction of activated larvacidal macrophages. Journal of Immunology 163, 3872–7.CrossRefGoogle Scholar
James, S. L. & Cheever, A. W. (1985). Comparison of immune responses between high and low responder mice in the concomitant immunity and vaccine models of resistance to Schistosoma mansoni. Parasitology 91, 301–15.CrossRefGoogle ScholarPubMed
Mangold, B. L. & Dean, D. A. (1986). Passive transfer with serum and IgG antibodies of irradiated cercariae-induced resistance against Schistosoma mansoni in mice. Journal of Immunology 136, 2644–8.CrossRefGoogle Scholar
McLaren, D. J., Pearce, E. J. & Smithers, S. R. (1985). Site potential for challenge attrition in mice, rats and guinea pigs vaccinated with irradiated cercariae of Schistosoma mansoni. Parasite Immunology 7, 29–44.CrossRefGoogle ScholarPubMed
McLaren, D. J. & Rogers, M. V. (1986). Schistosoma mansoni: liver phase challenge attrition is a stage-dependent phenomenon in guinea-pigs vaccinated with highly irradiated cercariae. Parasite Immunology 8, 307–18.CrossRefGoogle ScholarPubMed
McLaren, D. J. & Smithers, S. R. (1987). The immune response to schistosomes in experimental hosts. In The Biology of Schistosomes (ed. Rollinson, D. & Simpson, A. J. G.), pp. 233–63. London: Academic Press.Google Scholar
McLaren, D. J. & Smithers, S. R. (1988). Serum from CBA/Ca mice vaccinated with irradiated cercariae of Schistosoma mansoni protects naive recipients through the recruitment of cutaneous effector cells. Parasitology 97, 287–302.Google ScholarPubMed
Pearce, E. J. (1983). Studies on immunity to Schistosoma mansoni in the guinea pig. Ph.D. thesis, Brunei University.Google Scholar
Pearce, E. J. & McLaren, D. J. (1983). Schistosoma mansoni: in vivo and in vitro studies of immunity using the guinea-pig model. Parasitology 87, 465–79.CrossRefGoogle ScholarPubMed
Rogers, M. V. & McLaren, D. J. (1987). Analysis and comparison of immune reactivity in guinea-pigs immunized with equivalent numbers of normal or radiation-attenuated cercariae of Schistosoma mansoni. Parasitology 95, 43–59.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Gammage, K. (1980). The recovery of Schistosoma mansoni from the skin, lungs and hepatic portal system of naive mice and mice previously exposed to S. mansoni. Evidence for two phases of parasite attrition in immune mice. Parasitology 80, 289–300.CrossRefGoogle Scholar
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Vignali, D. A. A., Bickle, Q. D. & Taylor, M. G. (1988). Studies on immunity to Schistosoma mansoni in vivo: whole-body irradiation has no effect on vaccine-induced resistance in mice. Parasitology 96, 49–61.CrossRefGoogle ScholarPubMed
Ward, R. E. M. & McLaren, D. J. (1988). Schistosoma mansoni: evidence that eosinophils and/or macrophages contribute to skin-phase challenge attrition in vaccinated CBA/Ca mice. Parasitology 96, 63–84.CrossRefGoogle ScholarPubMed
Ward, R. E. M. & McLaren, D. J. (1989). Schistosoma mansoni: migration and attrition of challenge parasites in naive rats and rats protected with vaccine serum. Parasite Immunology 11, 125–46.CrossRefGoogle ScholarPubMed