Hostname: page-component-848d4c4894-ndmmz Total loading time: 0 Render date: 2024-04-30T18:31:22.085Z Has data issue: false hasContentIssue false
  • English
  • Français

Interferon-γ induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases

Published online by Cambridge University Press:  01 March 2000

M. HAMADIEN ,
M. BAKHIET  and
R. A. HARRIS
M. HAMADIEN
Affiliation:
Division for Infectious Diseases, Huddinge University Hospital, SE-141 86 Huddinge
M. BAKHIET
Affiliation:
Division for Infectious Diseases, Huddinge University Hospital, SE-141 86 Huddinge
R. A. HARRIS
Affiliation:
Neuroimmunology Unit, Centre for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden
Get access Rights & Permissions [Opens in a new window]

Abstract

Bidirectional signalling between Trypanosoma brucei brucei and CD8+ T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-γ. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-γ in a dose–response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-γ-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.

Keywords

Trypanosoma bruceiIFN-γtrypanosome-derived lymphocyte triggering factor (TLTF)
Type
Research Article
Information
Parasitology , Volume 120 , Issue 3 , March 2000 , pp. 281 - 287
Copyright
2000 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)