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The in vivo and in vitro ananlysis of immunity to Trichinella spiralis in mice and rats

Published online by Cambridge University Press:  06 April 2009

D. D. Despommier ,
W. C. Campbell  and
L. S. Blair
D. D. Despommier
Affiliation:
Division of Tropical Medicine, School of Public Health, Columbia University, 630 West 168th Street, New York 10032
W. C. Campbell
Affiliation:
Merck Institute for Therapeutic Research, Rahway, New Jersey 07065
L. S. Blair
Affiliation:
Merck Institute for Therapeutic Research, Rahway, New Jersey 07065
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Extract

Wistar-Furth strain male rats and CFW strain male mice were immunized against Trichinella spiralis using an antegenic fraction derived from a cell-free homogenate of mature muscle larvae.

In rats, animlas immunized with 250 μg of antigen harboured singnificantly fewer (135000) muscle larvae 30 days after oral challenge than controls (231000). Furhter 7-day-old adult worms derived from immunized rats shed 48% fewer (P < 0·001) newborn larvae over a 24 h period in vitro than adult worms from non-immunized animals.

Mice were injected with either 10 or 100 μg of antigen. In comparison with non-immunized controls, mice immunized with 100μg of antigen harboured significantly fewer adult worms at days 7 and 9 after oral challenge infection, while female worms recovered from immune mice on days 6–10 after challenge shed fewer newborn larvae in vitro. Finally, mice immunized with 100μg of antigen harboured significantly fewer (10391) muscle larvae at 30 days after challenge than did controls (47750).

Immunization of mice with 10μ of antigen did not induce a statistically significant reduction in adult worms at either days 7 or 9 after challenge (P < 0·5). However, adult female worms from mice receiving 10μg of antigen still shed fewer larvae than did adults from control mice (P < 0·05). Mice immunized with 10μg of antigen harboured significantly fewer (13700) recoverable muscle larvae than did controls at 30 days after challenge (39000).

Type
Research Article
Information
Parasitology , Volume 74 , Issue 1 , February 1977 , pp. 109 - 119
Copyright
Copyright © Cambridge University Press 1977

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