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Identification and quantification of rodent malaria strains and species using gene probes

Published online by Cambridge University Press:  06 April 2009

G. Snounou
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
T. Bourne
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
W. Jarra
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
S. Viriyakosol
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
K. N. Brown
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA

Extract

A DNA probe PCsv4 and a subclone thereof PCsv4.1, hybridize specifically to rodent malaria DNA. DNA purified from a small volume (10 μ1) of infected mouse blood was used to determine the composition of the parasite population present. The hybridization signal following PCsv4 probing of slot-blotted DNA correlated directly with parasitaemia. The hybridization pattern and Intensity, resulting from probing restriction enzyme digested and Southern-blotted genomic DNA, determined the identity of the infecting parasite line(s), and provided a semi-quantitative measure of parasite burden. Fifteen parasite lines representative of all four Plasmodium species infecting rodents can be differentiated in this way.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1992

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