Hostname: page-component-7479d7b7d-qs9v7 Total loading time: 0 Render date: 2024-07-13T13:25:48.291Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification and quantification of rodent malaria strains and species using gene probes

Published online by Cambridge University Press:  06 April 2009

G. Snounou ,
T. Bourne ,
W. Jarra ,
S. Viriyakosol  and
K. N. Brown
G. Snounou
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
T. Bourne
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
W. Jarra
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
S. Viriyakosol
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
K. N. Brown
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Extract

A DNA probe PCsv4 and a subclone thereof PCsv4.1, hybridize specifically to rodent malaria DNA. DNA purified from a small volume (10 μ1) of infected mouse blood was used to determine the composition of the parasite population present. The hybridization signal following PCsv4 probing of slot-blotted DNA correlated directly with parasitaemia. The hybridization pattern and Intensity, resulting from probing restriction enzyme digested and Southern-blotted genomic DNA, determined the identity of the infecting parasite line(s), and provided a semi-quantitative measure of parasite burden. Fifteen parasite lines representative of all four Plasmodium species infecting rodents can be differentiated in this way.

Keywords

Plasmodiumrodent malariaDNA probeRFLPdiagnosis.
Type
Research Article
Information
Parasitology , Volume 105 , Issue 1 , August 1992 , pp. 21 - 27
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Carter, R. (1978). Studies on enzyme variation in the murine malaria parasites Plasmodium berghei, P. yoelii, P. vinckei and P. chabaudi by starch gel electrophoresis. Parasitology 76, 241–67.CrossRefGoogle ScholarPubMed
Coombs, G. H. & Gutteridge, W. E. (1975). Growth in vitro and metabolism of Plasmodium vinckei chabaudi. Journal of Protozoology 22, 550–60.CrossRefGoogle ScholarPubMed
Feinberg, A. P. & Vogelstein, B. (1984). Addendum: A technique for labelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137, 266–7.Google ScholarPubMed
James, S. P., Nicol, W. D. & Shute, P. G. (1932). A study of induced malignant tertian malaria. Proceedings of the Royal Society of Medicine 25, 1153–86.CrossRefGoogle ScholarPubMed
Jarra, W. (1982). Studies on the induction and expression of protective immunity in rodent malaria. Ph.D. thesis, Brunel University, U.K.Google Scholar
Jarra, W. & Brown, K. N. (1985). Protective immunity to malaria: studies with cloned lines of Plasmodium chabaudi and P. berghei in CBA/Ca mice. I. The effectiveness and inter- and intra-species specificity of immunity induced by infection. Parasite Immunology 7, 595–606.CrossRefGoogle ScholarPubMed
Jarra, W. & Brown, K. N. (1989). Protective immunity to malaria: studies with cloned lines of rodent malaria in CBA/Ca mice. IV. The specificity of mechanisms resulting in crisis and resolution of the primary acute phase parasitaemia of Plasmodium chabaudi chabaudi and P. yoelii yoelii. Parasite Immunology 11, 1–13.CrossRefGoogle ScholarPubMed
Killick-Kendrick, R. (1978). Taxonomy, zoogeography and evolution. In Rodent Malaria, (ed. Killick-kendrick, R. & Peters, W.) pp. 1–52. London: Academic Press.Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbour, N. Y: Cold Spring Habour Laboratory.Google Scholar
Mulligan, H. W. & Sinton, J. A. (1933 a). Studies in immunity in malaria. Part II. Superinfection with various strains of monkey malaria parasites. Records of the Malaria Survey of India 3, 529–68.Google Scholar
Mulligan, H. W. & Sinton, J. A. (1933 b). Studies in immunity in malaria. Part III. Multiple superinfections with various strains of Plasmodium knowlesi. Records of the Malaria Survey of India 3, 809–39.Google Scholar
Snounou, G., Jarra, W., Viriyakosol, S., Wood, J. C. & Brown, K. N. (1989). Use of a DNA probe to analyse the dynamics of infection with rodent malaria parasites confirms that parasite clearance during crisis is predominantly strain-and species-specific. Molecular Biochemical Parasitology 37, 37–46.CrossRefGoogle ScholarPubMed
Thaithong, S., Beale, G. H., Fenton, B., McBride, J., Rosario, V., Walker, A. & Walliker, D. (1984). Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 242–5.CrossRefGoogle Scholar
Viriyakosol, S., Snounou, G. & Brown, K. N. (1989). The use of DNA probes for the differentiation of rodent malaria stains and species. Molecular Biochemical Parasitology 32, 93–100.CrossRefGoogle Scholar
Walliker, D. (1983). The genetic basis of diversity in malaria parasites. In Advances in Parasitology, Vol. 22, pp. 217–259. London: Academic Press.Google Scholar
Yoeli, M. & Most, H. (1965). Studies on sporozoite-induced infections of rodent malaria. I. The preerythrocytic tissue stage of Plasmodium berghei. American Journal of Tropical Medicine and Hygiene 14, 700–14.CrossRefGoogle ScholarPubMed