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A dipeptidylpeptidase secreted by Fasciola hepatica

Published online by Cambridge University Press:  06 April 2009

C. Carmona ,
S. McGonigle ,
A. J. Dowd ,
A. M. Smith ,
S. Coughlan ,
E. McGowran  and
J. P. Dalton
C. Carmona
Affiliation:
Unidad de Biologia Parasitaria, Instituto de Higiene, Universidad de la República, Montevideo, Uruguay
S. McGonigle
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
A. J. Dowd
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
A. M. Smith
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
S. Coughlan
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
E. McGowran
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
J. P. Dalton
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
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Summary

A dipeptidylpeptidase (DPP) was isolated from Fasciola hepatica by gel-filtration and ion-exchange chromatography. The exoproteinase is secreted by newly excysted juveniles, immature and mature flukes. The liver fluke DPP is a serine proteinase of molecular weight > 200 kDa and differs from previously characterized mammalian DPPs in its substrate preference and susceptibility to inactivation by inhibitors. The parasite DPP may function in the latter stages of the proteolytic digestion of host macromolecules. In this manner, the enzyme may be important in providing the parasite with dipeptides that could be absorbed through the intestine as nutrient.

Keywords

liver flukeFasciola hepaticaTrematodadipeptidylpeptidaseexopeptidase
Type
Research Article
Information
Parasitology , Volume 109 , Issue 1 , July 1994 , pp. 113 - 118
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Bogitsh, B. J. & Dresden, M. H. (1983). Fluorescent histochemistry of acid proteases in adult Schistosoma mansoni and Schistosoma japonicum. Journal of Parasitology 69, 106–10.CrossRefGoogle ScholarPubMed
Carmona, C., Dowd, A., Smith, A. & Dalton, J. P. (1993). Fasciola hepatica: cathepsin L proteinase secreted in vitro prevents antibody-mediated eosinophil attachment to newly excysted juveniles. Molecular and Biochemical Parasitology 62, 9–18.CrossRefGoogle ScholarPubMed
Chapman, C. B. & Mitchell, C. F. (1982). Proteolytic cleavage of immunoglobulin by enzymes released by Fasciola hepatica. Veterinary Parasitology 11, 165–78.CrossRefGoogle ScholarPubMed
Dalton, J. P. & Heffernan, M. (1989). Thiol proteases released in vitro by Fasciola hepatica. Molecular and Biochemical Parasitology 35, 161–6.CrossRefGoogle ScholarPubMed
Davies, D. A., Bogitsh, J. B. & Nunnally, D. A. (1969). Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. III. Nonspecific esterases in Haemalotoechus medioptexus. Experimental Parasitology 24, 121–9.CrossRefGoogle Scholar
McGinty, A., Moore, M., Halton, D. W. & Walker, B. (1993). Characterization of the cysteine proteinases of the common liver fluke Fasciola hepatica using novel, active-site directed affinity labels. Parasitology 106, 487–93.CrossRefGoogle ScholarPubMed
Neurath, H. (1989). The diversity of proteolytic enzymes. In Proteolytic Enzymes (ed. Beynon, R. J. & Bond, J. S.). Oxford, New York and Tokyo: IRL Press, Oxford University Press.Google Scholar
Redinbaugh, M. G. & Turley, R. B. (1986). Adaptation of the bicinchoninic acid protein assay for use with microtitre plates and sucrose gradient fractions. Analytical Biochemistry 153, 267–71.CrossRefGoogle ScholarPubMed
Rege, A. A., Herrera, P. R., Lopez, M. & Dresden, M. H. (1989). Isolation and characterization of a cysteine proteinase from Fasciola hepatica adult worms. Molecular and Biochemical Parasitology 35, 89–95.CrossRefGoogle ScholarPubMed
Sea-Hui, K. (1988). A novel dipeptidyl aminopeptidase in rat brain. Journal of Biological Chemistry 263, 6613–18.Google Scholar
Senft, A. W. (1976). Observations on the physiology of the gut of Schistosoma mansoni. In Biochemistry of Parasites and Host–Parasite Relationships (ed. Van den Bossche, H.), pp. 335–342. New York: North-Holland Publishing Co.Google Scholar
Smith, A. M., Dowd, A. J., Heffernan, M., Robertson, C. D. & Dalton, J. P. (1993 a). Fasciola hepatica: a secreted cathepsin L-like proteinase cleaves host immunoglobulin. International Journal for Parasitology 23, 977–83.CrossRefGoogle ScholarPubMed
Smith, A. M., Dowd, A. J., McGonigle, A., Keegan, P. S., Brennan, G., Trudgett, A. & Dalton, J. P. (1993 b). Purification of a cathepsin L-like proteinase secreted by adult Fasciola hepatica. Molecular and Biochemical Parasitology 62, 1–8.CrossRefGoogle ScholarPubMed