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An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal samples

Published online by Cambridge University Press:  02 June 2009

E. N. VIANNA ,
J. O. COSTA ,
C. K. S. SANTOS ,
M. C. CURY ,
E. F. SILVA ,
A. O. COSTA  and
M. A. GOMES
E. N. VIANNA
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627-31270-901, Belo Horizonte, Minas Gerais, Brazil
J. O. COSTA
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627-31270-901, Belo Horizonte, Minas Gerais, Brazil
C. K. S. SANTOS
Affiliation:
Laboratório de Parasitologia, ICB, Universidade Federal de Uberlândia, Minas Gerais, Brazil
M. C. CURY
Affiliation:
Laboratório de Parasitologia, ICB, Universidade Federal de Uberlândia, Minas Gerais, Brazil
E. F. SILVA
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627-31270-901, Belo Horizonte, Minas Gerais, Brazil
A. O. COSTA
Affiliation:
Departamento de Patologia Básica, SCB, Universidade Federal do Paraná, Curitiba, Paraná, Brazil
M. A. GOMES*
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627-31270-901, Belo Horizonte, Minas Gerais, Brazil
*
*Corresponding author: Departamento de Parasitologia, ICB-UFMG, Av. Antonio Carlos, 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil. Tel: +55 31 34092846. Fax: +55 31 34092970. E-mail: magomes@icb.ufmg.br
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Summary

Since it is known that Entamoeba dispar is non-pathogenic and morphologically similar to E. histolytica, there are many targets used in PCR for differentiating these species. However, obtaining high quality DNA from fecal samples is fundamental for PCR. Most methods are laborious or use kits that make diagnosis expensive. In the present work, a new simple, fast and cheap technique of DNA extraction from fecal samples was combined with a PCR for an episomal target in order to identify E. histolytica and E. dispar in feces.

Keywords

Entamoeba histolyticaEntamoeba dispardifferential diagnostic
Type
Research Article
Information
Parasitology , Volume 136 , Issue 7 , June 2009 , pp. 765 - 769
Copyright
Copyright © Cambridge University Press 2009

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