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Morphometric and genetic evidence for cryptic diversity in Gyrodactylus (Monogenea) infecting non-native European populations of Ameiurus nebulosus and A. melas

Published online by Cambridge University Press:  30 July 2020

Markéta Ondračková Open the ORCID record for Markéta Ondračková [Opens in a new window] ,
Mária Seifertová ,
Anna Bryjová ,
Eric Leis  and
Pavel Jurajda
Markéta Ondračková*
Affiliation:
Czech Academy of Sciences, Institute of Vertebrate Biology, Květná 8, 60365Brno, Czech Republic
Mária Seifertová
Affiliation:
Department of Botany and Zoology, Masaryk University, Faculty of Science, Kotlářská 2, 61137Brno, Czech Republic
Anna Bryjová
Affiliation:
Czech Academy of Sciences, Institute of Vertebrate Biology, Květná 8, 60365Brno, Czech Republic
Eric Leis
Affiliation:
U.S. Fish and Wildlife Service, La Crosse Fish Health Center-Midwest Fisheries Center, Onalaska, Wisconsin, USA
Pavel Jurajda
Affiliation:
Czech Academy of Sciences, Institute of Vertebrate Biology, Květná 8, 60365Brno, Czech Republic Faculty of Agronomy, Mendel University, Zemědělská 1/1665, 603 65Brno, Czech Republic
*
Author for correspondence: Markéta Ondračková, E-mail: audrey@sci.muni.cz
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Abstract

Gyrodactylid parasites were observed on non-native populations of North-American freshwater catfishes, Ameiurus nebulosus and Ameiurus melas (Siluriformes: Ictaluridae), at several sites in the Elbe River basin, Czech Republic, Europe. Using a combination of morphological and genetic analyses, the parasites infecting A. nebulosus were determined to be Gyrodactylus nebulosus, a North American parasite co-introduced to Europe along with its Ameiurus fish hosts. Subtle morphometrical differences, as well as seasonal variations, were observed among parasites collected from A. nebulosus and A. melas. The host-related variation was further supported through genetic analysis of the partial 18S rDNA, ITS1-5.8S-ITS2 and COI, showing 0.2, 3.0 and 4.8% divergence, respectively. Consistent genetic differences indicated there were two distinct genotypes. Subtle morphological differences associated with the shape of sickle toe, anchor root and ventral bar membrane, according to host species, also supported the description of a new cryptic species, Gyrodactylus melas n. sp., infecting A. melas. Multivariate morphometrical analysis of haptoral hard parts showed significant differences between the anchor lengths of G. nebulosus and G. melas n. sp. However, the measurements of the haptoral hard structures partially overlapped between species, limiting the usage of these parameters for species delineation.

Keywords

Bullhead catfishIctaluridaegyrodactylidsnon-native hostNorth-America
Type
Research Article
Information
Parasitology , Volume 147 , Issue 14 , December 2020 , pp. 1700 - 1711
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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