Skip to main content Accessibility help
×
Home
Hostname: page-component-79b67bcb76-pclkk Total loading time: 0.172 Render date: 2021-05-12T19:16:16.301Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true }

Article contents

Isolation of an aspartic proteinase precursor from the egg of a hard tick, Boophilus microplus

Published online by Cambridge University Press:  01 June 1998

C. LOGULLO
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690 Departamento de Biologia Celular e Molecular, IB-UFF, Outeiro São João Batista, S/N, Centro, Niterói, RJ, Brazil, C.P. 100180, CEP 24001-970
I. DA SILVA VAZ
Affiliation:
Centro de Biotecnologia do Estado do Rio Grande do Sul, UFRGS, Avenida Bento Gonçalves, 9500, prédio-II-A, Bloco A, Porto Alegre, RS, Brazil, C.P. 15005, CEP 91501-970 Faculdade de Veterinária, UFRGS, Avenida Bento Gonçalves, 9500, Porto Alegre, RS, Brazil, C.P. 15005, CEP 91501-970
M. H. F. SORGINE
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690
G. O. PAIVA-SILVA
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690
F. S. FARIA
Affiliation:
Departamento de Genética, IB-CCS-UFRJ, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690
R. B. ZINGALI
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690
M. F. R. DE LIMA
Affiliation:
Centro de Biotecnologia do Estado do Rio Grande do Sul, UFRGS, Avenida Bento Gonçalves, 9500, prédio-II-A, Bloco A, Porto Alegre, RS, Brazil, C.P. 15005, CEP 91501-970
L. ABREU
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690 Departamento de Biologia Celular e Molecular, IB-UFF, Outeiro São João Batista, S/N, Centro, Niterói, RJ, Brazil, C.P. 100180, CEP 24001-970
E. FIALHO OLIVEIRA
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690
E. W. ALVES
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690
H. MASUDA
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690
J. C. GONZALES
Affiliation:
Faculdade de Veterinária, UFRGS, Avenida Bento Gonçalves, 9500, Porto Alegre, RS, Brazil, C.P. 15005, CEP 91501-970
A. MASUDA
Affiliation:
Centro de Biotecnologia do Estado do Rio Grande do Sul, UFRGS, Avenida Bento Gonçalves, 9500, prédio-II-A, Bloco A, Porto Alegre, RS, Brazil, C.P. 15005, CEP 91501-970
P. L. OLIVEIRA
Affiliation:
Departamento de Bioquímica Médica, ICB-CCS-UFRJ, Bloco H, sala H2031, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-690

Abstract

An aspartic proteinase precursor, herein named BYC (Boophilus Yolk pro-Cathepsin) was isolated from eggs of the hard tick, Boophilus microplus. As judged by electrophoresis on sodium dodecyl sulfate polyacrylamide slab gel (SDS–PAGE), purified BYC presented 2 bands of 54 and 49 kDa, bearing the same NH2-terminal amino acid sequence. By Western blot analysis, BYC was also found in the haemolymph, indicating an extraovarian site of synthesis. Several organs were incubated in culture medium with [35S] methionine, and only the gut and fat body showed synthesis of BYC polypeptides. Protein sequencing of both the NH2-terminal and an internal sequence obtained after cyanogen bromide (CNBr) cleavage of BYC revealed homology with several aspartic proteinase precursors. Incubation at pH 3·5 resulted in autoproteolysis of BYC, which produced the mature form of the enzyme, that displayed pepstatin-sensitive hydrolytic activity against haemoglobin. Western blot analysis using anti-BYC monoclonal antibodies showed proteolytic processing of BYC during embryogenesis and suggested activation of the enzyme during development. A role of BYC in degradation of vitellin, the major yolk protein of tick eggs, is discussed.

Type
Research Article
Copyright
1998 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Isolation of an aspartic proteinase precursor from the egg of a hard tick, Boophilus microplus
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Isolation of an aspartic proteinase precursor from the egg of a hard tick, Boophilus microplus
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Isolation of an aspartic proteinase precursor from the egg of a hard tick, Boophilus microplus
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *