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In vitro test for the evaluation of the efficacy of topical products for the control of Cochliomyia hominivorax larvae

Published online by Cambridge University Press:  01 April 2020

Sabrina Nathália Louzada Nogueira
Affiliation:
Universidade de Franca (UNIFRAN), Av. Dr Armando Salles Oliveira, 201, Parque Universitário, Franca, SP, 14404-600, Brazil
Maria Fernanda da Silva
Affiliation:
Universidade de Franca (UNIFRAN), Av. Dr Armando Salles Oliveira, 201, Parque Universitário, Franca, SP, 14404-600, Brazil
Ricardo de Andrade Furtado
Affiliation:
Universidade de Franca (UNIFRAN), Av. Dr Armando Salles Oliveira, 201, Parque Universitário, Franca, SP, 14404-600, Brazil
Daniel Paulino Júnior
Affiliation:
Universidade de Franca (UNIFRAN), Av. Dr Armando Salles Oliveira, 201, Parque Universitário, Franca, SP, 14404-600, Brazil
Maria Cecília Soares
Affiliation:
Universidade Federal de Uberlândia, Campus Umuarama, Uberlândia, Minas Gerais, Brazil
Maria Mariana Alves Andrade
Affiliation:
Universidade de Franca (UNIFRAN), Av. Dr Armando Salles Oliveira, 201, Parque Universitário, Franca, SP, 14404-600, Brazil
Eduardo Gilberti Nascimento
Affiliation:
Universidade de Franca (UNIFRAN), Av. Dr Armando Salles Oliveira, 201, Parque Universitário, Franca, SP, 14404-600, Brazil
Lorena Lopes Ferreira
Affiliation:
Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Vando Edésio Soares
Affiliation:
Universidade Brasil, Descalvado, São Paulo, Brazil
Welber Daniel Zanetti Lopes*
Affiliation:
Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil
Rafael Paranhos de Mendonça
Affiliation:
Universidade de Franca (UNIFRAN), Av. Dr Armando Salles Oliveira, 201, Parque Universitário, Franca, SP, 14404-600, Brazil
*
Author for correspondence: Welber Daniel Zanetti Lopes, E-mail: wdzlopes@hotmail.com

Abstract

Cochliomyia hominivorax larvae cause myiasis in animals and humans. To register a commercial product to control this dipteran is necessary to experiment on animals. The in vitro test was standardized to evaluate the larvicidal efficacy of commercial topical products. Five formulations were analysed in vitro and in vivo. For the in vitro test, a colony was formed and three replicates (n = 200) of each larval stage (L1, L2 and L3) were treated. The viability of the larvae was evaluated after 5 and 30 min, and at 1, 2, 6, 12, 24, 48, 60 and 72 h post-treatment (HPT). For the in vivo test, 30 bovines divided into six groups were castrated to achieve natural infestation with C. hominivorax. Animals in the treated groups received the product. Myiasis and efficacy were evaluated 12, 24, 36, 48, 60 and 72 HPT. Four formulations tested in the in vitro test achieved 100% efficacy at 24 HPT. In the in vivo experiment only one achieved 100% efficacy at 24 HPT. However, all products achieved the maximum efficacy by the end of study. The in vitro test developed here could be adopted to evaluate the efficacy of topical products for the control of C. hominivorax larvae.

Type
Research Article
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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