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Genetic diversity of cervid Trypanosoma theileri in Honshu sika deer (Cervus nippon) in Japan

Published online by Cambridge University Press:  27 July 2021

Imron Rosyadi ,
Aogu Setsuda ,
Mafie Eliakunda ,
Ai Takano ,
Ken Maeda ,
Atsuko Saito-Ito ,
Kazuo Suzuki  and
Hiroshi Sato Open the ORCID record for Hiroshi Sato [Opens in a new window]
Imron Rosyadi
Affiliation:
Laboratory of Parasitology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
Aogu Setsuda
Affiliation:
Department of Pathological and Preventive Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
Mafie Eliakunda
Affiliation:
Department of Pathological and Preventive Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
Ai Takano
Affiliation:
Department of Pathological and Preventive Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan Division of Pathogenic Microorganisms, Research Center for Thermotolerant Microbial Resources, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
Ken Maeda
Affiliation:
Department of Pathological and Preventive Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan Division of Pathogenic Microorganisms, Research Center for Thermotolerant Microbial Resources, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
Atsuko Saito-Ito
Affiliation:
Section of Microbiology, School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe, Hyogo650-8530, Japan
Kazuo Suzuki
Affiliation:
Hikiiwa Park Center, 1629 Inari-cho, Tanabe, Wakayama646-0051, Japan
Hiroshi Sato*
Affiliation:
Laboratory of Parasitology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan Department of Pathological and Preventive Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan Division of Pathogenic Microorganisms, Research Center for Thermotolerant Microbial Resources, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
*
Author for correspondence: Hiroshi Sato, E-mail: sato7dp4@yamaguchi-u.ac.jp
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Abstract

The taxonomy of ruminant Trypanosoma theileri and its relatives (Kinetoplastida: Trypanosomatidae) is controversial, with recent phylogenetic studies segregating T. theileri in cattle and other ruminants worldwide into two major genetic lineages (the TthI and TthII clades) based on genetic markers. In the present study, T. theileri-like trypanosomes isolated from Honshu sika deer (Cervus nippon) in the western Japan (YMG isolate) were genetically characterized using a number of genetic markers. Sika deer trypanosomes of the YMG isolate were genetically different from the Trypanosoma sp. TSD1 isolate previously recorded from Hokkaido sika deer in northern Japan, with the former trypanosome isolate being genetically closer to European cervid trypanosomes and the bovine T. theileri TthII lineage. In contrast, the latter isolate exhibited greater relatedness to North American cervid trypanosomes and the bovine T. theileri TthI lineage, although a clear genetic distinction between these was apparent. Furthermore, trypanosomes in Honshu sika deer from the central part of Japan harboured additional genetic diversity and were closer to either TSD1 or YMG isolates, while distinct from known T. theileri-related genotypes. Importantly, cervids and wild ruminants worldwide might harbour divergent descendants of a T. theileri ancestor, which exhibit rigid host specificity to either bovines or cervid species.

Keywords

Cervus nippongenetic diversitygGAPDHJapanMegatrypanumphylogenyrDNAsika deertaxonomyTrypanosoma theileri
Type
Research Article
Information
Parasitology , Volume 148 , Issue 13 , November 2021 , pp. 1636 - 1647
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These two authors contributed equally to this work.

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