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Genetic characterization of Toxoplasma gondii in Iranian HIV positive patients using multilocus nested-PCR-RFLP method

Published online by Cambridge University Press:  18 November 2019

Seyed Abdollah Hosseini ,
Mehdi Sharif ,
Shahabeddin Sarvi ,
Saeid Abediankenari ,
Mohammad Bagher Hashemi-Soteh ,
Afsaneh Amouei ,
Mahboobeh Montazeri ,
Sargis A. Aghayan ,
Sara Gholami  and
Davoud Shaker
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Seyed Abdollah Hosseini
Affiliation:
Toxoplasmois Research Center, Mazandaran University of Medical Sciences, Sari, Iran Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Mehdi Sharif
Affiliation:
Department of Parasitology, School of Medicine, Sari Branch, Islamic AZAD University, Sari, Iran
Shahabeddin Sarvi
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Saeid Abediankenari
Affiliation:
Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, IR, Iran
Mohammad Bagher Hashemi-Soteh
Affiliation:
Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran
Afsaneh Amouei
Affiliation:
Toxoplasmois Research Center, Mazandaran University of Medical Sciences, Sari, Iran Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Mahboobeh Montazeri
Affiliation:
Toxoplasmois Research Center, Mazandaran University of Medical Sciences, Sari, Iran Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Sargis A. Aghayan
Affiliation:
Laboratory of Zoology, Research Institute of Biology, Yerevan State University, Yerevan, Armenia
Sara Gholami
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Davoud Shaker
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Azadeh Mizani
Affiliation:
Toxoplasmois Research Center, Mazandaran University of Medical Sciences, Sari, Iran Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Shafigheh Shabanzadeh
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Ahmad Daryani*
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
*
Author for correspondence: Ahmad Daryani, E-mail: daryanii@yahoo.com
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Abstract

The aim of the present study was to investigate the prevalence and genotyping of Toxoplasma gondii in Iranian human immunodeficiency virus (HIV)-positive patients using multilocus-nested polymerase chain reaction restriction fragment length polymorphism (Mn-PCR-RFLP). A total of 102 serum samples obtained from infected patients were collected from the laboratory centres in northern Iran. Anti-T. gondii antibodies and deoxyribonucleic acid (DNA) detection were accomplished by an enzyme-linked immunosorbent assay and PCR. The Mn-PCR-RFLP method was used for the genotyping of T. gondii. Overall, 68.6% (70/102) and 11.7% (12/102) of the individuals were tested positive for anti-T. gondii immunoglobulin G and T. gondii DNA, respectively. Complete genotyping was performed on 10/12 (83.3%) PCR-positive samples. Accordingly, the samples were classified as genotype #1 (type II clonal; n = 3, 30%), genotype #2 (type III clonal; n = 2, 20%), genotype #10 (type I clonal; n = 2, 20%), genotype #27 (type I variant; n = 1, 10%), genotype #35 (type I variant; n = 1, 10%) and genotype #48 (type III variant; n = 1, 10%). The results were indicative of the high frequency of the type I and type I variant of T. gondii strains in HIV-positive patients in northern Iran. Given the high prevalence of T. gondii and frequency of pathogenic types (pathogen in laboratory mice) in the patients, special measures should be taken to prevent the possible increased incidence of encephalitis by T. gondii.

Keywords

ELISAgenotypeHIVIranMn-PCR-RFLPToxoplasma gondii
Type
Research Article
Information
Parasitology , Volume 147 , Issue 3 , March 2020 , pp. 322 - 328
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Copyright
Copyright © Cambridge University Press 2019

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