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Diphenyl diselenide and sodium selenite associated with chemotherapy in experimental toxoplasmosis: influence on oxidant/antioxidant biomarkers and cytokine modulation

Published online by Cambridge University Press:  08 August 2014

Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
Department of Small Animal Clinical, Universidade Federal de Santa Maria, Brazil
Department of Animal Science, Universidade do Estado de Santa Catarina, Brazil
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
Department of Chemistry, Universidade Federal de Santa Maria, Brazil
Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Brazil
Department of Clinical and Toxicological Analysis, Universidade Federal de Santa Maria, Brazil
Universidade do Extremo Sul Catarinense, UNESC, Brazil
Universidade do Extremo Sul Catarinense, UNESC, Brazil
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
Universidade do Extremo Sul Catarinense, UNESC, Brazil
Department of Chemistry, Universidade Federal de Santa Maria, Brazil
Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Brazil
Universidade Luterana do Brasil, ULBRA, Brazil
Department of Clinical and Toxicological Analysis, Universidade Federal de Santa Maria, Brazil
Department of Small Animal Clinical, Universidade Federal de Santa Maria, Brazil
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
*Corresponding author: Departamento de Clínica de Pequenos animais/LacVet – Laboratório de Análises Clínicas Veterinária – Hospital Veterinário UFSM Sala 108, Universidade Federal de Santa Maria (UFSM), Campus Universitário, CEP 97105-900, Santa Maria, RS, Brazil. E-mail:


The aim of this study was to assess the effect of sulfamethoxazole/trimethoprim (ST) supplemented with diphenyl diselenide and sodium selenite in experimental toxoplasmosis, on oxidant/antioxidant biomarkers and cytokine levels. Eighty-four BALB/c mice were divided in seven groups: group A (negative control), and groups B to G (infected). Blood and liver samples were collected on days 4 and 20 post infection (p.i.). Levels of thiobarbituric acid (TBA) reactive substances and advanced oxidation protein products (AOPP) were assessed in liver samples. Both biomarkers were significantly increased in infected groups on day 4 p.i., while they were reduced on day 20 p.i., compared with group A. Glutathione reductase (GR) activity significantly (P<0·01) increased on day 4 p.i., in group G, compared with group A. INF-γ was significantly increased (P<0·001) in both periods, day 4 (groups B, C, F and G) and 20 p.i. (groups C, F and G). IL-10 significantly reduced (P<0·001) on day 4 p.i. in group B; however, in the same period, it was increased (P<0·001) in groups C and G, compared with group A. On day 20 p.i., IL-10 increased (P<0·001) in groups F and G. Therefore, our results highlighted that these forms of selenium, associated with the chemotherapy, were able to reduce lipid peroxidation and protein oxidation, providing a beneficial immunological balance between the production of pro- and anti-inflammatory cytokines.

Research Article
Copyright © Cambridge University Press 2014 

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