Skip to main content Accessibility help
×
Home
Hostname: page-component-768ffcd9cc-mqrwx Total loading time: 0.292 Render date: 2022-11-29T22:29:23.010Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "displayNetworkTab": true, "displayNetworkMapGraph": false, "useSa": true } hasContentIssue true

Article contents

Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis

Published online by Cambridge University Press:  21 November 2016

ALESSANDRA L. MORASSUTTI*
Affiliation:
Laboratório de Biologia Parasitária da Faculdade de Biociências e Laboratório de Parasitologia Molecular do Instituto de Pesquisas Biomédicas da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av Ipiranga 6690, 90690-900 Porto Alegre RS, Brasil
LISA N. RASCOE
Affiliation:
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, Georgia 30329, USA
SUKWAN HANDALI
Affiliation:
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, Georgia 30329, USA
ALEXANDRE J. DA SILVA
Affiliation:
U.S. Food & Drug Administration, Division of Virulence Assessment, Center for Foods Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Silver Spring, Maryland, USA
PATRICIA P. WILKINS
Affiliation:
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, Georgia 30329, USA
CARLOS GRAEFF-TEIXEIRA
Affiliation:
Laboratório de Biologia Parasitária da Faculdade de Biociências e Laboratório de Parasitologia Molecular do Instituto de Pesquisas Biomédicas da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av Ipiranga 6690, 90690-900 Porto Alegre RS, Brasil
*
*Corresponding author: Instituto de Pesquisas Biomédicas da PUCRS, Avenida Ipiranga 6690, 2 andar, Sala 20, CEP: 90690-900 Porto Alegre RS, Brazil. E-mail: almorassutti@gmail.com

Summary

The primary causative agent of eosinophilic meningoencephalitis (EoM) in endemic regions is the nematode Angiostrongylus cantonensis. The occurrence of EoM was previously restricted to countries in Southeast Asia and the Pacific Islands; however, more recently, it has been reported from other regions, including Brazil. The commonly used diagnosis is detection of specific antibody reactivity to the 31 kDa antigen, which is derived from female worm somatic extracts. Here we report the occurrence of cross-reactivity to this antigen in sera from other parasitic infections, especially those that may cause EoM, such as gnathostomiasis, toxocariasis, hydatidosis and strongyloidiasis. We also demonstrated that the cross-reactivity, in part, is dependent of the concentration of antigen used in Western blot assays. We discuss the importance of these findings on the interpretation of this test.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aguiar Prieto, P. H., Pascual Gispert, J., Dumenigo, B., Perera De Puga, G. and Gálvez Oviedo, M. D. (1981). Angiostrongylus cantonensis. Intermediate hosts in the 2 Havana provinces. Revista Cubana de Medicina Tropical 33, 173177.Google ScholarPubMed
de Savigny, D. H., Voller, A. and Woodruff, A. W. (1979). Toxocariasis: serological diagnosis by enzyme immunoassay. Journal of Clinical Pathology 32(3), 284288.CrossRefGoogle ScholarPubMed
Eamsobhana, P., Tungtrongchitr, A., Wanachiwanawin, D., Yong, H. S. and Mak, J. W. (1998). Characterization of 31 kda specific Antigen from Parastrongylus cantonensis (Nematoda: Metastrongylidae). Journal of International Medical Research 2(1), 912.Google Scholar
Eamsobhana, P., Yoolek, A. and Suvouttho, S. (2001). Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels. Southeast Asian Journal of Tropical Medicine and Public Health 32, 308313.Google ScholarPubMed
Espírito-Santo, M. C., Pinto, P. L., Mota, D. J. and Gryschek, R. C. (2013). The first case of Angiostrongylus cantonensis eosinophilic meningitis diagnosed in the city of São Paulo, Brazil. Revista do Instituto de Medicina Tropical de São Paulo 55, 129132.CrossRefGoogle ScholarPubMed
Graeff-Teixeira, C., Da Silva, A. C. and Yoshimura, K. (2009). Update on eosinophilic meningoencephalitis and its clinical relevance. Clinical and Microbiology Reviews 22, 322348. Table of Contents.CrossRefGoogle ScholarPubMed
Kim, D. Y., Stewart, T. B., Bauer, R. W. and Mitchell, M. (2002). Parastrongylus (=Angiostrongylus) cantonensis now endemic in Louisiana wildlife. Journal for Parasitology 88, 10241026.CrossRefGoogle ScholarPubMed
Jiang, L., Xu, X. N., Li, X., Xue, H. C. and Feng, Z. (2004). Identification of the immunodominant regions of the Em18 antigen and improved serodiagnostic specificity for alveolar echinococcosis. Parasite Immunology 10, 377385.CrossRefGoogle Scholar
Jin, E., Ma, D., Liang, Y., Ji, A. and Gan, S. (2005). MRI findings of eosinophilic myelomeningoencephalitis due to Angiostrongylus cantonensis. Clinical Radiology 60, 242250.CrossRefGoogle ScholarPubMed
Jin, E. H., Ma, Q., Ma, D. Q., He, W., Ji, A. P. and Yin, C. H. (2008). Magnetic resonance imaging of eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis following eating freshwater snails. Chinese Medical Jounal 121, 6772.Google ScholarPubMed
Lima, A. R., Mesquita, S. D., Santos, S. S., Aquino, E. R., Rosa, L. A. R., Duarte, F. S., Teixeira, A. O., Costa, Z. R. and Ferreira, M. L. (2009). Alicata disease: neuroinfestation by Angiostrongylus cantonensis in Recife, Pernambuco, Brazil. Arquivos de Neuro-Psiquiatria 67, 10931096.CrossRefGoogle ScholarPubMed
Lindo, J. F., Escoffery, C. T., Reid, B., Codrington, G., Cunningham-Myrie, C. and Eberhard, M. L. (2004). Fatal autochthonous eosinophilic meningitis in a Jamaican child caused by Angiostrongylus cantonensis . American Journal of Tropical Medicine and Hygiene 70, 425428.Google Scholar
Morassutti, A. L., Levert, K., Perelygin, A., Da Silva, A. J., Wilkins, P. and Graeff-Teixeira, C. (2012). The 31-kDa antigen of Angiostrongylus cantonensis comprises distinct antigenic glycoproteins. Vector Borne and Zoonotic Diseases 12, 961968.CrossRefGoogle ScholarPubMed
Morassutti, A. L., Perelygin, A., Levert, K., Lin, S. C., Lee, Y. M., Da Silva, A. J., Wilkins, P. P. and Graeff-Teixeira, C. (2013). Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report. Hawai'i Journal of Medicine & Public Health 72, 5862.Google ScholarPubMed
Morassutti Al Thiengo, Sc, Fernandez, M., Sawanyawisuth, K. and Graeff-Teixeira, C. (2014). Eosinophilic meningitis caused by Angiostrongylus cantonensis: an emergent disease in Brazil. Memorias do Instituto Oswaldo Cruz 109(4), 399407.CrossRefGoogle Scholar
Nuamtanong, S. (1996). The evaluation of the 29 and 31 kDa antigens in female A. cantonensis for serodiagnosis of human angiostrongyliasis. Southeast Asian Journal of Tropical Medicine and Public Health 27, 291296.Google ScholarPubMed
Qvarnstrom, Y., Xayavong, M., da Silva, A. C., Park, S. Y., Whelen, A. C., Calimlim, P. S., Sciulli, R. H., Honda, S. A., Higa, K., Kitsutani, P., Chea, N., Heng, S., Johnson, S., Graeff-Teixeira, C., Fox, L. M. and da Silva, A. J. (2016). Real-Time polymerase chain reaction detection of Angiostrongylus cantonensis DNA in cerebrospinal fluid from patients with eosinophilic meningitis. American Journal of Tropical Medicine and Hygiene 94(1), 176181. doi: 10.4269/ajtmh.15–0146.CrossRefGoogle ScholarPubMed
Sawanyawisuth, K., Takahashi, K., Hoshuyama, T., Senthong, V., Limpawattana, P., Intapan, P. M., Wilson, D., Tiamkao, S., Jitpimolmard, S. and Chotmongkol, V. (2009). Clinical factors predictive of encephalitis caused by Angiostrongylus cantonensis . American Journal of Tropical Medicine and Hygiene 81, 698701.CrossRefGoogle ScholarPubMed
Slom, T. J., Cortese, M. M., Gerber, S. I., Jones, R. C., Holtz, T. H., Lopez, A. S., Zambrano, C. H., Sufit, R. L., Sakolvaree, Y., Chaicumpa, W., Herwaldt, B. L. and Johnson, S. (2002). An outbreak of eosinophilic meningitis caused by Angiostrongylus cantonensis in travelers returning from the Caribbean. New England Journal of Medicine 346, 668675.CrossRefGoogle ScholarPubMed
Veríssimo, C. M., Morassutti, A. L., von Itzstein, M., Sutov, G., Hartley-Tassell, L., McAtamney, S., Dell, A., Haslam, S. M. and Graeff-Teixeira, C. (2016). Characterization of the N-glycans of female Angiostrongylus cantonensis worms. Experimental Parasitology 166, 137143. doi: 10.1016/j.exppara.2016.04.012 CrossRefGoogle ScholarPubMed
Wang, Q. P., Lai, D. H., Zhu, X. Q., Chen, X. G. and Lun, Z. R. (2008). Human angiostrongyliasis. Lancet Infectious Diseases 8, 621630.CrossRefGoogle ScholarPubMed
9
Cited by

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *