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The combined treatment of praziquantel with osteopontin immunoneutralization reduces liver damage in Schistosoma japonicum-infected mice

Published online by Cambridge University Press:  06 February 2012

BO-LIN CHEN
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
GUI-YING ZHANG*
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
SHI-PING WANG
Affiliation:
Department of Parasitology, College of Xiangya Basic Medicine, Central South University, Changsha, 410008, Hunan Province, China
QIAN LI
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
MEI-HUA XU
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
YUE-MING SHEN
Affiliation:
Department of Gastroenterology, Changsha Central Hospital, Changsha, 410004, Hunan Province, China
LU YAN
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
HUAN GU
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
JIA LI
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
Y. L. HUANG
Affiliation:
Division of Phamaceutical Science, School of Pharmacy, University of Auckland, Private Bag 92019, Auckland, New Zealand
YI-BING MU
Affiliation:
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China
*
*Corresponding author: Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province,China. Tel: +86 1387 3186462. Fax: +86 0731 84327321. E-mail: guiyingzhang@hotmail.com

Summary

The aim of this study was to evaluate the therapeutic effects of osteopontin neutralization treatment on schistosome-induced liver injury in BALB/C mice. We randomly divided 100 BALB/C mice into groups A, B, C, D and group E. Mice in all groups except group A were abdominally infected with schistosomal cercariae to induce a schistosomal hepatopathological model. Mice in group C, D and group E were respectively administered with praziquantel, praziquantel plus colchicine and praziquantel plus neutralizing osteopontin antibody. We extracted mouse liver tissues at 3 and 9 weeks after the ‘stool-eggs-positive’ day, observed liver histopathological changes by haematoxylin-eosin and Masson trichrome staining and detected the expression of osteopontin, alpha-smooth muscle actin (α-SMA) and transforming growth factor-beta (TGF-β1) by immunohistochemistry, RT-PCR and Western blot. We found that praziquantel plus neutralizing osteopontin antibody treatment significantly decreased the granuloma dimension, the percentage of collagen and the expression of osteopontin, α-SMA and TGF-β1 compared to praziquantel plus colchicine treatment in both the acute and chronic stage of schistosomal liver damage (P<0·05). So we believe that the combined regimen of osteopontin immunoneutralization and anti-helminthic treatment can reduce the granulomatous response and liver fibrosis during the schistosomal hepatopathologic course.

Type
Research Article
Copyright
Published by Cambridge University Press 2012

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The combined treatment of praziquantel with osteopontin immunoneutralization reduces liver damage in Schistosoma japonicum-infected mice
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