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Carvacryl acetate nanoencapsulated with chitosan/chichá gum exhibits reduced toxicity in mice and decreases the fecal egg count of sheep infected with gastrointestinal nematodes

Published online by Cambridge University Press:  09 July 2021

Weibson P. P. André
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
José R. P. Junior
Affiliation:
Laboratório de Química Analítica e Ambiental, Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará, Fortaleza, Brazil
Géssica S. Cavalcante
Affiliation:
Laboratório de Química de Produtos Naturais, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Wesley L. C. Ribeiro
Affiliation:
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Brazil
José V. Araújo-Filho
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Selene M. Morais
Affiliation:
Laboratório de Química de Produtos Naturais, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Lorena M. B. Oliveira
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Flávia O. M. S. Abreu
Affiliation:
Laboratório de Química Analítica e Ambiental, Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará, Fortaleza, Brazil
Claudia M. L. Bevilaqua*
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
*
Author for correspondence: Claudia M. L. Bevilaqua, E-mail: bevilaqua.uece@gmail.com

Abstract

The nanoencapsulation of biocomposites with anthelmintic action has been proposed as an alternative for improving their efficiency. Thus, the current study aimed to evaluate the efficacy of carvacryl acetate nanoencapsulated with biopolymers (nCVA) in the control of sheep gastrointestinal nematodes. CVA was nanoencapsulated with chitosan/chichá gum and characterized in terms of its efficacy of encapsulation (EE), yield and zeta potential. The acute toxicity of nCVA was evaluated in mice. For the fecal egg count reduction test, 40 animals were divided into four groups (n = 10) and orally administered the following treatments: G1, 250 mg kg−1 CVA; G2, 250 mg kg−1 nCVA; G3, chitosan/chichá gum (negative control) and G4, 2.5 mg kg−1 monepantel (positive control). Feces were collected on days 0 and 16 posttreatment to determine the eggs per gram of feces (epg). The EE and yield of nCVA were 72.8 and 57.5%, respectively. The nanoparticles showed a size of 764.5 ± 302.5 nm, and the zeta potential at pH 3.2 was +22.0 mV. nCVA presented a 50% lethal dose (LD50) of 2609 mg kg−1. By 16 days posttreatment, CVA, nCVA and monepantel reduced the epg by 52.9.7, 71.5 and 98.7%, respectively, and the epg of sheep treated with nCVA differed from that of the negative control (P > 0.05) but did not differ from that of sheep treated with CVA. In conclusion, the nanoencapsulation of CVA reduced its toxicity, and nCVA showed anthelmintic activity.

Type
Research Article
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Abreu, FOMS, Bianchini, C, Forte, MMC and Kist, TBL (2008) Influence of the composition and preparation method on the morphology and swelling behavior of alginate–chitosan hydrogels. Carbohydrate Polymers 74, 283289.CrossRefGoogle Scholar
Adams, RP, (2007) Identification of Essential Oil Components by Gás Chromatography/Mass Spectrometry. Carol Stream: Allured Publishing Corporation. p. 804.Google Scholar
Andre, WPP, Ribeiro, WLC, Cavalcante, GS, Santos, JML, Macedo, ITF, Paula, HCB, Freitas, RM, Maia, SM, Melo, JV and Bevilaqua, CML (2016) Comparative efficacy and toxic effects of carvacryl acetate and carvacrol on sheep gastrointestinal nematodes and mice. Veterinary Parasitology 218, 5258.CrossRefGoogle ScholarPubMed
Andre, WPP, Junior, JRP, Cavalcante, GS, Ribeiro, WLC, Araújo-Filho, JV, Santos, JML, Alves, APNN, Monteiro, JP, Morais, SM, Silva, ING, Oliveira, LMB, Abreu, FOMS and Bevilaqua, CML (2020a) Anthelmintic activity of nanoencapsulated carvacryl acetate against gastrointestinal nematodes of sheep and its toxicity in rodents. Revista Brasileira de Parasitologia Veterinária 29, e013119.CrossRefGoogle Scholar
Andre, WPP, Junior, JRP, Cavalcante, GS, Ribeiro, WLC, Araújo-Filho, JV, Cavalcanti, BC, Morais, SM, Oliveira, LMB, Bevilaqua, CML and Abreu, FOMS (2020b) Chitosan nanoparticles loaded with carvacrol and carvacryl acetate for improved anthelmintic activity. Journal of the Brazilian Chemical Society 31, 16141622.Google Scholar
Araújo-Filho, JV, Ribeiro, WLC, André, WPP, Cavalcante, GS, Guerra, MCM, Muniz, CR, Macedo, ITF, Rondon, FCM, Bevilaqua, CML and Oliveira, LMB (2018) Effects of Eucalyptus citriodora essential oil and its major component, citronellal, on Haemonchus contortus isolates susceptible and resistant to synthetic anthelmintics. Industrial Crops and Products 124, 294299.CrossRefGoogle Scholar
Brito, ACF, Silva, DA, De Paula, RCM and Feitosa, JPA (2004) Sterculia striata exudate polysaccharide: characterization, rheological properties and comparison with Sterculia urens (karaya) polysaccharide. Polymer International 53, 10251032.CrossRefGoogle Scholar
Cabaret, J (2014) Reliable phenotypic evaluations of anthelmintic resistance in herbivores: how and when should they be done? In. Quick, W (ed.), Anthelmintics – Clinical Pharmacology, Uses in Veterinary Medicine and Efficacy. New York: Nova Science Publisher, pp. 126.Google Scholar
Camurça-Vasconcelos, AL, Bevilaqua, CM, Morais, SM, Maciel, MV, Costa, CT, Macedo, IT, Oliveira, LMB, Braga, RR, Silva, RA and Vieira, LS (2007) Anthelmintic activity of Croton zehntneri and Lippia sidoides essential oils. Veterinary Parasitology 148, 288294.CrossRefGoogle ScholarPubMed
Coles, GC, Jackson, F, Pomroy, WE, Prichard, RK, Von Samson-Himmelstjerna, G, Silvestre, A, Taylor, MA and Vercruysse, J (2006) The detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 136, 167185.CrossRefGoogle ScholarPubMed
Dash, KM, Hall, K and Barger, IA (1988) The role of arithmetic and geometric mean worm egg counts in faecal egg count reduction tests and in monitoring strategic drenching programs in sheep. Australian Veterinary Journal 65, 6668.CrossRefGoogle ScholarPubMed
Duarte, ER, Matias, AD, Bastos, GA, Maia, RC, Júnior, VSM and Soares, ACM (2019) Anthelmintic efficacy of trichlorfon and blood parameters of young lambs infected with Haemonchus contortus. Veterinary Parasitology 272, 4043.CrossRefGoogle Scholar
Hoste, H, Torres-Acosta, JF, Alonso-Diaz, MA, Brunet, S, Sandoval-Castro, C and Adote, SH (2008) Identification and validation of bioactive plants for the control of gastrointestinal nematodes in small ruminants. Tropical Biomedicine 25, 5672.Google ScholarPubMed
Katiki, LM, Barbieri, AME, Araujo, RC, Veríssimo, CJ, Louvandini, H and Ferreira, JFS (2017) Synergistic interaction of ten essential oils against Haemonchus contortus in vitro. Veterinary Parasitology 243, 4751.CrossRefGoogle ScholarPubMed
Katiki, LM, Araujo, RC, Ziegelmeyer, L, Gomes, ACP, Gutmanis, G, Rodrigues, L, Bueno, MS, Veríssimo, CJ, Louvandini, H, Ferreira, JFS and Amarante, AFT (2019) Evaluation of encapsulated anethole and carvone in lambs artificially- and naturally-infected with Haemonchus contortus. Experimental Parasitology 197, 3642.CrossRefGoogle ScholarPubMed
Keawchaoon, L and Yoksan, R (2011) Preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles. Colloids and Surfaces B: Biointerfaces 84, 163171.CrossRefGoogle ScholarPubMed
Luo, Y and Wang, Q (2014) Recent development of chitosan-based polyelectrolyte complexes with natural polysaccharides for drug delivery. International Journal of Biological Macromolecules 64, 353367.CrossRefGoogle ScholarPubMed
Macedo, ITF, Oliveira, LMB, André, WPP, Araújo Filho, JV, Santos, JML, Rondon, FCM, Ribeiro, WLC, Camurça-Vasconcelos, ALF, Oliveira, EF, Paula, HCB and Bevilaqua, CML (2019) Anthelmintic effect of Cymbopogon citratus essential oil and its nanoemulsion on sheep gastrointestinal nematodes. Revista Brasileira de Parasitologia Veterinária 28, 522527.CrossRefGoogle ScholarPubMed
Magalhães, GA Jr, Neto, EM, Sombra, VG, Richter, AR, Abreu, CMWS, Feitosa, JPA, Paula, HCB, Goycoolea, FM and Paula, RCM (2016). Chitosan/Sterculia striata polysaccharides nanocomplex as a potential chloroquine drug release device. International Journal of Biological Macromolecules 88, 244253.CrossRefGoogle ScholarPubMed
Mangas, MBP, Mello, VM and Suarez, PAZ (2013) Thermal behavior of Sterculia striata oil. Thermochimica Acta 564, 3942.CrossRefGoogle Scholar
Mesquita, MA, Silva Júnior, JB, Panassol, AM, Oliveira, EF, Camurça-Vasconcelos, ALF, Paula, HCB and Bevilaqua, CML (2013) Anthelmintic activity of Eucalyptus staigeriana encapsulated oil on sheep gastrointestinal nematodes. Parasitology Research 112, 31613165.CrossRefGoogle Scholar
Nixon, SA, Welz, C, Woods, DJ, Costa-Junior, L, Zamanian, M and Martin, RJ (2020) Where are all the anthelmintics? Challenges and opportunities on the path to new anthelmintics. International Journal for Parasitology: Drugs and Drug Resistance 14, 816.Google ScholarPubMed
Novato, T, Gomes, GA, Zeringóta, V, Franco, CT, Oliveira, DR, Melo, D, Carvalho, MG, Daemon, E and Monteiro, CMO (2018) In vitro assessment of the acaricidal activity of carvacrol, thymol, eugenol and their acetylated derivatives on Rhipicephalus microplus (Acari: Ixodidae). Veterinary Parasitology 260, 14.CrossRefGoogle Scholar
OECD (2008) Organization for Economic Cooperation and Development. Guidelines for the testing of chemicals: Repeated Dose 28–Day Oral Toxicity Study in Rodents. Paris: OECD, Guideline 407.Google Scholar
Paula, HCB, Oliveira, EF, Carneiro, MJM and Paula, RCM (2016) Matrix effect on the spray drying nanoencapsulation of Lippia sidoides essential oil in chitosan-native bum blends. Planta Medica 22, 17.Google Scholar
Ribeiro, JC, Ribeiro, WLC, Camurça-Vasconcelos, ALF, Macedo, ITF, Santos, JML, Paula, HCB, Aráujo-Filho, JV, Magalhães, RD and Bevilaqua, CML (2014) Efficacy of free and nanoencapsulated Eucalyptus citriodora essential oils on sheep gastrointestinal nematodes and toxicity for mice. Veterinary Parasitology 204, 243248.CrossRefGoogle ScholarPubMed
Ribeiro, WL, Camurça-Vasconcelos, AL, Macedo, IT, Santos, JM, Araújo-Filho, JV, Ribeiro, JC, Pereira, VA, Viana, DA, Paula, HC and Bevilaqua, CM (2015) In vitro effects of Eucalyptus staigeriana nanoemulsion on Haemonchus contortus and toxicity in rodents. Veterinary Parasitology 212, 444447.CrossRefGoogle ScholarPubMed
Ribeiro, WLC, Camurça-Vasconcelos, ALF, Santos, JML, Macedo, ITF, Ribeiro, JC, Oliveira, EF, Paula, HCB and Bevilaqua, CML (2017) The use of Eucalyptus staigeriana nanoemulsion for control of sheep haemonchosis. Pesquisa Veterinária Brasileira 37, 221226.CrossRefGoogle Scholar
Roberts, FHS and O'Sullivan, JP (1950) Methods for egg counts and larval cultures for strongyles infesting the gastrointestinal tract of cattle. Australian Journal of Agricultural Research 1, 99102.CrossRefGoogle Scholar
Sallé, G, Doyle, SR, Cortet, J, Cabaret, J, Berriman, M, Holroyd, N and Cotton, JA (2019) The global diversity of Haemonchus contortus is shaped by human intervention and climate. Nature Communications 10, 4811.CrossRefGoogle ScholarPubMed
Santos, JML, Vasconcelos, JF, Frota, GA, Freitas, EP, Teixeira, M, Vieira, Bevilaqua CML and Monteiro, JP (2019) Quantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus. Experimental Parasitology 205, 107734.CrossRefGoogle ScholarPubMed
Sharifi-Rad, M, Varoni, EM, Iriti, M, Martorell, M, Setzer, WN, Contreras, MM, Salehi, B, Soltani-Nejad, A, Rajabi, S, Tajbakhsh, M and Sharifi_Rad, J (2018) Carvacrol and human health: a comprehensive review. Phytotherapy Research 32, 16751687.CrossRefGoogle ScholarPubMed
Silva, FF, Bezerra, HMFF, Feitosa, TF and Vilela, VLR (2018) Nematode resistance to five anthelmintic classes in naturally infected sheep herds in Northeastern Brazil. Revista Brasileira de Parasitologia Veterinária 27, 423429.CrossRefGoogle Scholar
Sombra, FM, Richter, AR, Araújo, AR, Ribeiro, FOS, Mendes, JFS, Fontenelle, ROS, Silva, DA, Paula, HCB, Feitosa, JPA, Goycoolea, FM and Paula, RCM (2019) Nanocapsules of Sterculia striata acetylated polysaccharide as a potential monomeric amphotericin B delivery matrix. International Journal of Biological Macromolecules 130, 655663.CrossRefGoogle Scholar
Van Wyk, JA and Mayhew, E (2013) Morphological identification of parasitic nematode infective larvae of small ruminants and cattle: a practical lab guide. Onderstepoort Journal of Veterinary Research 80, 539.CrossRefGoogle ScholarPubMed
Ways, TMM, Lau, WM and Khutoryanskiy, VV (2018) Chitosan and its derivatives for application in mucoadhesive drug delivery systems. Polymers 10, 267.CrossRefGoogle Scholar

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Carvacryl acetate nanoencapsulated with chitosan/chichá gum exhibits reduced toxicity in mice and decreases the fecal egg count of sheep infected with gastrointestinal nematodes
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