Lipids were extracted from needles of Norway spruce plants of different provenances. The plants, seeds of which were originally collected from all over Europe, were grown at two sites in England at the same longitude but one 350 km north of the other. Significant correlations were observed between monogalactosyl diglyceride (MGDG) and digalactosyl diglyceride (DGDG) ratios on the one hand and, on the other, the lowest extreme winter temperature at the original site of seed collection (EWTS).

In the case of lipid changes, however, environmental differences due to site were more important than the inherent genotypic characteristics of the planted trees while provenances with the lowest EWTS values had more MGDG than DGDG. This implies that existing winter hardiness under mild climatic conditions does not come from changes in this ratio although DGDG formation from MGDG is known to be an important feature of winter hardening in Norway spruce elsewhere in Europe. However, closer analysis of the fatty acids in lipids from provenances at individual sites did reveal significant correlations between those provenances with low EWTS values and certain molar amounts or ratios of fatty acids in DGDG, some of which could be traced to genotypic expression. These partly involved the unusual Δ5 desaturations which are mainly confined to the fatty acids of those conifers like Norway spruce that harden to very low temperatures.