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Screening of basidiomycetes and xylariaceous fungi for lignin peroxidase and laccase gene-specific sequences

Published online by Cambridge University Press:  15 February 2005

Stephen B. POINTING ,
Anna L. PELLING ,
Gavin J. D. SMITH ,
Kevin D. HYDE  and
C. Adinaryana REDDY
Stephen B. POINTING
Affiliation:
Department of Ecology and Biodiversity, University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China. E-mail: pointing@hkucc.hku.hk
Anna L. PELLING
Affiliation:
Department of Biochemistry, University of Hong Kong, Sassoon Road, Hong Kong, People's Republic of China.
Gavin J. D. SMITH
Affiliation:
Department of Ecology and Biodiversity, University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China. E-mail: pointing@hkucc.hku.hk
Kevin D. HYDE
Affiliation:
Department of Ecology and Biodiversity, University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China. E-mail: pointing@hkucc.hku.hk
C. Adinaryana REDDY
Affiliation:
Department of Microbiology and Molecular Genetics, and NSF Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824-4320, USA.
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Abstract

Lignin peroxidase and laccase gene-specific PCR primers were used to screen 38 diverse basidiomycetes and xylariaceous fungi. Lignin peroxidase gene-specific sequences were obtained for basidiomycetes only and were highly divergent. Possession of laccase genes was relatively widespread among basidiomycetes, and is shown for the first time in Xylariaceae. All sequences were highly conserved with no variation resulting in changes to predicted amino acid sequence. Those basidiomycetes shown to possess lignin peroxidase and laccase genes also produced the enzyme in vitro. Conversely none of the xylariaceous fungi shown to possess laccase genes were able to do so, whilst others decolorized Poly R yet yielded no PCR amplicons.

Type
Research Article
Information
Mycological Research , Volume 109 , Issue 1 , January 2005 , pp. 115 - 124
Copyright
© The British Mycological Society 2005

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