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Isolation, characterisation and expression patterns of a RAD51 ortholog from Pleurotus ostreatus

Published online by Cambridge University Press:  02 September 2002

Thomas S. P. MIKOSCH ,
Anton S. M. SONNENBERG  and
Leo J. L. D. VAN GRIENSVEN
Thomas S. P. MIKOSCH
Affiliation:
Glaucus Proteomics B.V., P.O. Box 54, 3980 CB Bunnik, The Netherlands. E-mail: tm@glaucusprot.com
Anton S. M. SONNENBERG
Affiliation:
PPO, Mushroom Research Unit, P.O. Box 6042, 5960 AA Horst, The Netherlands
Leo J. L. D. VAN GRIENSVEN
Affiliation:
PRI, P.O. Box 16, 6700 AA Wageningen, The Netherlands.
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Abstract

Using degenerated primers for conserved regions of RecA homologs we have isolated a gene from Pleurotus ostreatus that shows characteristic features of RAD51 homologs. The encoded amino acid sequence of P. ostreatusRAD51 (PoRAD51) shows greatest sequence similarities with RAD51 from Coprinus cinereus (89% identity). Furthermore the genomic organisation of PoRAD51 is almost identical to that of RAD51 from C. cinereus. Northern analysis shows that the expression of PoRAD51 is found in vegetative mycelium, and fruit body tissue, and that it is expressed at elevated levels in lamellae/basidia and following DNA damage. A sporulation deficient mutant strain of P. ostreatus (ATTC 58937) showed expression patterns of the RAD51 gene that are similar those of the normal sporulating strain.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 6 , June 2002 , pp. 682 - 687
Copyright
© The British Mycological Society 2002

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