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A genetic linkage map of Lentinula edodes (shiitake) based on AFLP markers

Published online by Cambridge University Press:  24 October 2002

Kazuhisa TERASHIMA ,
Teruyuki MATSUMOTO ,
Eiji HAYASHI  and
Yukitaka FUKUMASA-NAKAI
Kazuhisa TERASHIMA
Affiliation:
Japan Science and Technology Corporation (JST), Honmachi 4-1-8, Kawaguchi, Saitama 332-0012, Japan. The Tottori Mycological Institute, Kokoge 211, Tottori 689-1125, Japan.
Teruyuki MATSUMOTO
Affiliation:
The Tottori Mycological Institute, Kokoge 211, Tottori 689-1125, Japan.
Eiji HAYASHI
Affiliation:
Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan.
Yukitaka FUKUMASA-NAKAI
Affiliation:
The Tottori Mycological Institute, Kokoge 211, Tottori 689-1125, Japan.
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Abstract

A medium-dense genetic linkage map of Lentinula edodes (shiitake) was constructed based on 203 amplified fragment length polymorphism (AFLP) markers and two mating type factors. The segregation of these markers was generated from 95 progeny of a single cross of two distantly related strains. The map consists of 11 linkage groups comprised of eight large (over 100 centimorgans (cM)) and three small (less than 100 cM) groups, and the total genetic distance of the map was 1956.7 cM. The average rate of physical size to genetic distance could be roughly estimated to be less than 18.4 kb cM−1, which is low compared to the values obtained for other filamentous fungi. Seventeen of the AFLP markers showed highly distorted segregation ratios (χ2 values >6.63; P<0.01), and many of these were mapped in LG II (6 markers) and IV (6 markers).

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 8 , August 2002 , pp. 911 - 917
Copyright
© The British Mycological Society 2002

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Footnotes

Contribution No. 355 of The Tottori Mycological Institute.