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Genetic exchange in Metarhizium anisopliae strains co-infecting Phaedon cochleariae is revealed by molecular markers

Published online by Cambridge University Press:  01 April 2000

Soraya C. M. LEAL-BERTIOLI ,
Tariq M. BUTT ,
John F. PEBERDY  and
David J. BERTIOLI
Soraya C. M. LEAL-BERTIOLI
Affiliation:
Entomology and Nematology Department, IACR Rothamsted, Harpenden, Herts, AL5 2JQ, UK EMBRAPA/CENARGEN, SAIN Parque Rural, CP 02372, Brasília, DF, Brazil
Tariq M. BUTT
Affiliation:
Entomology and Nematology Department, IACR Rothamsted, Harpenden, Herts, AL5 2JQ, UK School of Biological Sciences, University Wales, Swansea, Singleton Park, Swansea SA2 8PP, UK
John F. PEBERDY
Affiliation:
School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
David J. BERTIOLI
Affiliation:
Entomology and Nematology Department, IACR Rothamsted, Harpenden, Herts, AL5 2JQ, UK EMBRAPA/CENARGEN, SAIN Parque Rural, CP 02372, Brasília, DF, Brazil
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Abstract

Recombination between two strains of Metarhizium anisopliae, subcultured on artificial medium and passed through an insect host was investigated. When grown on agar culture, or passed through a host, strains did not show detectable genetic changes. When passed through an insect in the presence of the other strain, genotypic changes in the isolates, as detected by esterase electrophoresis, restriction digestion of total genomic DNA and RAPD-PCR, were apparent. The instability of molecular markers suggests recombination between strains.

Type
Research Article
Information
Mycological Research , Volume 104 , Issue 4 , April 2000 , pp. 409 - 414
Copyright
© The British Mycological Society 2000

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