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Electrophoretic characterization of chitinases as a tool for the identification of Trichoderma harzianum strains

Published online by Cambridge University Press:  01 March 1998

HEDVA SCHICKLER ,
BAT CHEN DANIN-GEHALI ,
SHOSHAN HARAN  and
ILAN CHET
HEDVA SCHICKLER
Affiliation:
The Kennedy-Leigh Center for Horticultural Research
BAT CHEN DANIN-GEHALI
Affiliation:
Otto Warburg Center for Agricultural Biotechnology, the Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Quality Sciences, Rehovot 76100, Israel
SHOSHAN HARAN
Affiliation:
Otto Warburg Center for Agricultural Biotechnology, the Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Quality Sciences, Rehovot 76100, Israel
ILAN CHET
Affiliation:
Otto Warburg Center for Agricultural Biotechnology, the Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Quality Sciences, Rehovot 76100, Israel
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Abstract

The identification of Trichoderma strains is important for their application as biocontrol agents. We used a two-dimensional analysis, in which extracellular proteins of Trichoderma harzianum strains T-35, Y and TM were first separated according to their isoelectric point and then according to their molecular mass. Chitinase activities were detected in situ after the second separation. Each of the three strains exhibited a unique pattern of 3–5 different chitinases (1 or 2 N-acetyl-β-glucosaminidases, and 2 or 4 endochitinases). These unique profiles can be used to differentiate among strains within this species. The suggested procedure makes use of a property of T. harzianum that is directly involved in the mycoparasitic interaction, making it suitable for specific biocontrol applications.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 3 , March 1998 , pp. 373 - 377
Copyright
The British Mycological Society 1998

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