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Comparative analysis of polygalacturonases in isolates of seven species of Fusarium from Pinus pinea

Published online by Cambridge University Press:  20 February 2001

Martha L. POSADA
Affiliation:
Departamento de Microbiologia III, Facultad de Biología, Universidad Complutense, 28040-Madrid, Spain
Belén PATIÑO
Affiliation:
Departamento de Microbiologia III, Facultad de Biología, Universidad Complutense, 28040-Madrid, Spain
Salvador MIRETE
Affiliation:
Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040-Madrid, Spain
M. Carmen MUÑOZ
Affiliation:
Departamento de Zoologia, Enfermedades y Plagas Vegetales, Escuela Universitaria de Ingeniería Técnica Forestal, Universidad Politécnica, 28040-Madrid, Spain. E-mail: tegonja@eucmax.sim.ucm.es
Covadonga VÁZQUEZ
Affiliation:
Departamento de Microbiologia III, Facultad de Biología, Universidad Complutense, 28040-Madrid, Spain
M. Teresa GONZÁLEZ-JAÉN
Affiliation:
Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040-Madrid, Spain
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Abstract

Polygalacturonases (PGs) are important pectolytic enzymes produced by phytopathogenic fungi during the process of infection and colonisation of the host plants. In this work, PGs produced by isolates of seven Fusarium species associated to Pinus pinea have been analysed for: activity, isoform pattern observed by isoelectric focusing, endo- or exo-mode of action and their production in cultures growing on pectin and galacturonic acid. Of the seven isolates, those of F. oxysporum and F. moniliforme exhibited high PG activity and the most complex isoform patterns including acidic ones. These isolates were also more efficient in degrading the PG substrate, during which both endo and exo type polygalacturonase activities were detected. It is suggested that these features could be useful to the fungus during infection and colonisation of its host, specially during seed germination and early development.

Type
Research Article
Copyright
© The British Mycological Society 2001

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