Hostname: page-component-77c89778f8-fv566 Total loading time: 0 Render date: 2024-07-21T05:46:23.212Z Has data issue: false hasContentIssue false
  • English
  • Français

RAPD-PCR polymorphism and vegetative compatibility group variation in Spanish isolates of Acremonium cucurbitacearum

Published online by Cambridge University Press:  01 September 1999

M. J. VICENTE ,
D. CIFUENTES ,
J. L. CENIS  and
P. ABAD
M. J. VICENTE
Affiliation:
Dept. de Producción Vegetal, Universidad Politécnica de Valencia, Camino de Vera 14, 46020 Valencia, Spain
D. CIFUENTES
Affiliation:
Dept. Ingeniería Aplicada, Universidad de Murcia, Alfonso XIII 34, 30203 Cartagena, Spain
J. L. CENIS
Affiliation:
Centro de Investigacion y Desarrollo Agroalimentario (CIDA), 30150 La Alberca, Murcia, Spain
P. ABAD
Affiliation:
Dept. de Producción Vegetal, Universidad Politécnica de Valencia, Camino de Vera 14, 46020 Valencia, Spain
Get access

Abstract

Muskmelon crops have sustained serious losses in Spain since 1980, due to a disease characterized by a sudden collapse of the plant as the fruit approaches maturity. The cause has been attributed to Acremonium cucurbitacearum. To evaluate the genetic variation of A. cucurbitacearum in Spain and its relationship with other species of Acremonium, a study of vegetative compatibility groups (VCGs) and random amplified polymorphic DNA (RAPD-PCR) was carried out on 29 isolates from diseased muskmelon plants. The species used in comparison with A. cucurbitacearum were A. charticola, A. strictum, A. kiliense, A. sclerotigenum and A. crotocinigenum. Spanish isolates of A. cucurbitacearum were grouped in eight different VCGs, with two isolates belonging to more than one VCG. In only two of the reference species was it possible to complete all of the complementation tests: A. charticola (VCG 100) and A. crotocinigenum (VCG 200). The isolates of A. sclerotigenum and A. kiliense appeared to be naturally resistant to chlorate and could not be tested for VCG. All strains of A. strictum assayed were heterokaryon self-incompatible. The RAPD data showed considerable genetic variation, with 14 isolates grouped in 11 haplotypes. They also supported the recognition of A. cucurbitacearum as a new species, as the genetic similarity between A. cucurbitacearum and the reference species was less than 0·05.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 9 , September 1999 , pp. 1173 - 1178
Copyright
© The British Mycological Society 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)