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Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota)

Published online by Cambridge University Press:  14 June 2005

Francisco Adriano DE SOUZA
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Agrobiologia (CNPAB), Caixa Postal 74505, CEP 23851-970, Rodovia BR 465-RJ, km 7, Seropédica, Rio de Janeiro State, Brazil. E-mail: fdesouza@cnpab.embrapa.br
Stéphane DECLERCK
Affiliation:
Université Catholique de Louvain, Mycothèque de l'Université catholique de Louvain (MUCL*), Unité de microbiologie, 3 Place Croix du Sud, 1348 Louvain-La-Neuve, Belgium.
Eric SMIT
Affiliation:
National Institute for Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection, P.O. Box 1, NL-3720 BA, Bilthoven, The Netherlands.
George A. KOWALCHUK
Affiliation:
Netherlands Institute of Ecology (NIOO-KNAW), Centre for Terrestrial Ecology, Boterhoeksestraat 48, 6666 GA Heteren, The Netherlands.
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Abstract

The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular approaches. Spore ontogenesis was studied using Ri T-DNA transformed carrot roots and observations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreported pattern of outer spore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE), targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S. reticulata in a cluster together with S. cerradensis and S. heterogama, species that share similar spore wall organization and also possess ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA polymorphisms in four out of six Scutellospora species tested, and demonstrated that SSU nrDNA intragenomic polymorphism could be used as a marker to differentiate several closely related Scutellospora species.

Type
Research Article
Copyright
© The British Mycological Society 2005

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Footnotes

Part of the Belgian Coordinated Collections of Micro-organisms (BCCM).

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