Wear and fatigue are important factors in determining the reliability of microelectromechanical systems (MEMS). While the reliability of MEMS has received extensive attention, the physical mechanisms responsible for these failure modes have yet to be conclusively determined. In our work, we use a combination of on-chip testing methodologies and electron microscopy observations to investigate these mechanisms. Our previous studies have shown that fatigue in polysilicon structural thin films is a result of a ‘reaction-layer’ process, whereby high stresses induce a room-temperature mechanical thickening of the native oxide at the root of a notched cantilever beam, which subsequently undergoes moisture-assisted cracking. Devices from a more recent fabrication run are fatigued in ambient air to show that the post-release oxide layer thicknesses that were observed in our earlier experiments were not an artifact of that particular batch of polysilicon. New in vacuo data show that these silicon films do not display fatigue behavior when the post release oxide is prevented from growing, because of the absence of oxygen. Additionally, we are using polysilicon MEMS side-wall friction test specimens to study active mechanisms in sliding wear at the microscale. In particular, we have developed in vacuo and in situ experiments in the scanning electron microscope, with the objective of eventually determining the mechanisms causing both wear development and debris generation.