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TEM Investigation of U6+ and Re7+ Reduction by Desulfovibrio desulfuricans, a Sulfate-Reducing Bacterium

Published online by Cambridge University Press:  10 February 2011

Huifang Xu ,
Larry L. Barton ,
Pengchu Zhang  and
Yifeng Wang
Huifang Xu
Affiliation:
Department of Earth and Planetary Sciences, The University of New Mexico, Albuquerque, New Mexico87131. E-mail: hfxu@unm.edu
Larry L. Barton
Affiliation:
Department of Biology, The University of New Mexico, Albuquerque, New Mexico87131
Pengchu Zhang
Affiliation:
Sandia National Laboratories, 115 North Main Street, Carlsbad, New Mexico88220, USA
Yifeng Wang
Affiliation:
Sandia National Laboratories, 115 North Main Street, Carlsbad, New Mexico88220, USA
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Abstract

Uranium and its fission product Tc in aerobic environment will be in the forms of UO22+ and TcO4−. Reduced forms of tetravalent U and Tc are sparingly soluble. As determined by transmission electron microscopy, the reduction of uranyl acetate by immobilized cells of Desulfovibrio desuifuricans results in the production of black uraninite nanocrystals precipitated outside the cell. Some nanocrystals are associated with outer membranes of the cell as revealed from cross sections of these metabolic active sulfate-reducing bacteria. The nanocrystals have an average diameter of 5 nm and have anhedral shape. The reduction of Re7+ by cells of Desulfovibrio desulfuricans is fast in the media containing H2 electron donor, and slow in the media containing lactic acid. It is proposed that cytochrome in these cells has an important role in the reduction of uranyl and Re7+ that is a chemical analogue for one uranium fission product Tc7+ through transferring electron from molecular hydrogen or lactic acid to the oxyions of UO22+ and ReO4-.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 608: Symposium QQ – Scientific Basis for Nuclear Waste Management XXIII , 1999 , 299
Copyright
Copyright © Materials Research Society 2000

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