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Phosphate-dependent DNA Immobilization on Hafnium Oxide for Bio-Sensing Applications

Published online by Cambridge University Press:  31 January 2011

Nicholas M Fahrenkopf ,
Serge Oktyabrsky ,
Eric Eisenbraun ,
Magnus Bergkvist ,
Hua Shi  and
Nathaniel C Cady
Nicholas M Fahrenkopf
Affiliation:
nfahrenkopf@uamail.albany.edu, College of Nanoscale Science and Engineering, Albany, New York, United States
Serge Oktyabrsky
Affiliation:
SOktyabrsky@uamail.albany.edu, College of Nanoscale Science and Engineering, Albany, New York, United States
Eric Eisenbraun
Affiliation:
eEisenbraun@uamail.albany.edu, College of Nanoscale Science and Engineering, Albany, New York, United States
Magnus Bergkvist
Affiliation:
mBergkvist@uamail.albany.edu, College of Nanoscale Science and Engineering, Albany, New York, United States
Hua Shi
Affiliation:
hshi@albany.edu, University at Albany, Biological Sciences, Albany, New York, United States
Nathaniel C Cady
Affiliation:
ncady@uamail.albany.edu, College of Nanoscale Science and Engineering, Albany, New York, United States
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Abstract

Hafnium(IV) oxide (HfO2) has replaced silicon oxide as a gate dielectric material in leading edge CMOS technology, providing significant improvement in gate performance for field effect transistors (FETs). We are currently exploring this high-k dielectric for its use in nucleic acid-based FET biosensors. Due to its intrinsic negative charge, label-free detection of DNA can be achieved in the gate region of high-sensitivity FET devices. Previous work has shown that phosphates and phosphonates coordinate specifically onto metal oxide substrates including aluminum and titanium oxides. This property can therefore be exploited for direct immobilization of biomolecules such as nucleic acids. Our work demonstrates that 5’ phosphate-terminated single stranded DNA (ssDNA) can be directly immobilized onto HfO2 surfaces, without the need for additional chemical modification or crosslinking. Non-phosphorylated ssDNA does not form stable surface interactions with HfO2, indicating that immobilization is dependent upon the 5’ terminal phosphate. Further work has shown that surface immobilized ssDNA can be hybridized to complementary target DNA and that sequence-based hybridization specificity is preserved. These results suggest that the direct DNA-HfO2 immobilization strategy can enable nucleic acid-based biosensing assays on HfO2 terminated surfaces. This work will further enable high sensitivity electrical detection of biological targets utilizing transistor-based technologies.

Keywords

HfsensorP
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1191: Symposium OO – Materials and Strategies for Lab-on-a-Chip–Biological Analysis, Cell-Material Interfaces and Fluidic Assembly of Nanostructures , 2009 , 1191-OO12-04
Copyright
Copyright © Materials Research Society 2009

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References

[1] Kamahori, M. Ishige, Y. and Shimoda, M. “Enzyme Immunoassay Using a Reusable Extended-gate Field-Effect-Transistor Sensor with a Ferrocenylalkanethiol-modified Gold Electrode,” Analytical Sciences, vol. 24, pp. 1073–1079, 2008.10.2116/analsci.24.1073Google Scholar
[2] Goncalves, D. Prazeres, D. M. F. Chu, V. and Conde, J. P. “Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors,” Biosensors and Bioelectronics, vol. 24, pp. 545–551, 2008.10.1016/j.bios.2008.05.006Google Scholar
[3] Migita, S. Ozasa, K. Tanaka, T. and Haruyama, T. “Enzyme-based Field-Effect Transistor for Adenosine Triphosphate (ATP) Sensing,” Analytical Sciences, vol. 23, pp. 4, 2007.10.2116/analsci.23.45Google Scholar
[4] Kang, S. J. P. B. S. Chen, J. J. Ren, F. Johnson, J. W. Therrien, R. J. Rajagopal, P. Roberts, J. C. Piner, E. L. and Linthicum, K. J. “Electrical detection of deoxyribonucleic acid hybridization with AlGaN/GaN high electron mobility transistors,” Applied Physics Letters, vol. 89, pp. 122102–122104, 2006.10.1063/1.2354491Google Scholar
[5] Zhang, Q. and Subramanian, V. “DNA hybridization detection with organic thin film transistors: Toward fast and disposable DNA microarray chips,” Biosensors and Bioelectronics, vol. 22, pp. 3182–3187, 2007.10.1016/j.bios.2007.02.015Google Scholar
[6] IM, X. J. H. Hyungsoon, Gu, Bonsang and Choi, Yang-Kyu, “A dielectricmodulated field-effect transistor for biosensing,” Nature Nanotechnology, vol. 2, pp. 5, 2007.10.1038/nnano.2007.180Google Scholar
[7] Cheng, Y. Xiong, P. Yun, C. S. Strouse, G. F. Zheng, J. P. Yang, R. S. and Wang, Z. L. “Mechanism and Optimization of pH Sensing Using SnO2 Nanobelt Field Effect Transistors,” Nano. Lett., vol. 8, pp. 4179–4184, 2008.10.1021/nl801696bGoogle Scholar
[8] Yoon, H. Kim, J. Lee, N. Kim, B. and Jang, J. “A Novel Sensor Platform Based on Aptamer-Conjugated Polypyrrole Nanotubes for Label-Free Electrochemical Protein Detection,” Chembiochem, vol. 9, pp. 634–641, 2008.10.1002/cbic.200700660Google Scholar
[9] Xuan, G. Kolodzey, J. Kapoor, V. and Gonye, G. “Characteristics of field-effect devices with gate oxide modification by DNA,” Applied Physics Letters, vol. 87, pp. 103903–103905, 2005.10.1063/1.2041826Google Scholar
[10] Sakata, T. Kamahori, M. and Miyahara, Y. “DNA Analysis Chip Based on Field-Effect Transistors,” Japanese Journal of Applied Physics, vol. 44, pp. 2854–2859, 2005.10.1143/JJAP.44.2854Google Scholar
[11] Baur, G. S. B. Hernando, J. Purrucker, O. Tanaka, M. Nickel, B. Stutzmann, M. and Eickhoff, M. “Chemical functionalization of GaN and AlN surfaces,” Applied Physics Letters, vol. 87, pp. 263901–263903, 2005.10.1063/1.2150280Google Scholar
[12] Bujoli, B. Lane, S. M. Nonglaton, G. Pipelier, M. Leger, J. Talham, D. R. and Tellier, C. “Metal Phosphonates Applied to Biotechnologies: A Novel Approach to Oligonucleotide Microarrays,” Chem. Eur. J., vol. 11, pp. 1980–1989, 2005.10.1002/chem.200400960Google Scholar
[13] Rao, K. S. Rani, S. U. Charyulu, D. K. Kumar, K. N. Lee, H. and Kawai, T. “A novel route for immobilization of oligonucleotides onto modified silica nanoparticles,” Analytica Chimica Acta, vol. 576, pp. 7, 2006.10.1016/j.aca.2006.06.019Google Scholar
[14] Jespersen, M. L. Inman, C. E. Kearns, G. J. Foster, E. W. and Hutchison, J. E. “Alkanephosphonates on Hafnium-Modified Gold: A New Class of Self-Assembled Organic Monolayers,” J. AM. CHEM. SOC., vol. 129, pp. 5, 2007.10.1021/ja065598aGoogle Scholar
[15] Xu, X. Jindal, V. Shahedipour-Sandvik, F., Bergkvist, M. and Cady, N. C. “Direct immobilization and hybridization of DNA on group III nitride semiconductors” Applied Surface Science, vol. 255, pp. 5905–5909, 2009.10.1016/j.apsusc.2009.01.029Google Scholar
[16] Rashband, W. S. “Image J,” Bethesda, MD: US National Institutes of Health, 1997-2008.Google Scholar