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A Novel Microwave Device Designed to Preserve Cell Structure in Milliseconds

Published online by Cambridge University Press:  28 February 2011

Gary R. Login ,
Susan Kissell ,
Barbara K. Dwyer  and
Ann M. Dvorak
Gary R. Login
Affiliation:
Department of Pathology, Harvard School of Dental Medicine, Boston, MA 02115 Departments of Pathology, Harvard Medical School, and Beth Israel Hospital, and the Charles A. Dana Research Institute, Boston, MA 02215
Susan Kissell
Affiliation:
Departments of Pathology, Harvard Medical School, and Beth Israel Hospital, and the Charles A. Dana Research Institute, Boston, MA 02215
Barbara K. Dwyer
Affiliation:
Department of Pathology, Harvard School of Dental Medicine, Boston, MA 02115
Ann M. Dvorak
Affiliation:
Departments of Pathology, Harvard Medical School, and Beth Israel Hospital, and the Charles A. Dana Research Institute, Boston, MA 02215
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Abstract

We describe an innovative microwave instrument, designed in collaboration with and owned by Raytheon Company. The instrument permits the manipulation of biological specimens in their fluid milieu during the actual period of rapid tissue fixation. The specimen chamber is designed for sample containers up to 1.7 cm in diameter and 4.5 cm in height. Reflected power is reproducibly low, limiting the need for pretuning the microwave output to the sample. Microwave exposure can be controlled in 1 msecond increments with a range of 10 mseconds to 10 seconds. Mammalian cells and tissues fixed by this microwave device were evaluated by light and electron microscopy. Preliminary findings show large regions of excellent preservation in tissues and in cell suspensions in -100 mseconds.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 189: Symposium L – Microwave Processing of Materials II , 1990 , 329
Copyright
Copyright © Materials Research Society 1991

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References

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