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A Novel Electrospun Dendrimer-Gelatin Hybrid Nanofiber Scaffold for Tissue Regeneration and Drug Delivery

Published online by Cambridge University Press:  01 February 2011

Alicia P Smith-Freshwater ,
Gary L Bowlin  and
Hu Yang
Alicia P Smith-Freshwater
Affiliation:
smithfreshap@vcu.edu, Virginia Commonwealth University, Department of Biomedical Engineering, Richmond, VA, 23284, United States
Gary L Bowlin
Affiliation:
glbowlin@vcu.edu, Virginia Commonwealth University, Department of Biomedical Engineering, Richmond, VA, 23284, United States
Hu Yang
Affiliation:
hyang2@vcu.edu, Virginia Commonwealth University, Biomedical Engineering, 701 West Grace Street, PO Box 843067, Richmond, VA, 23284, United States
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Abstract

Gelatin has been widely used to develop tissue engineering scaffolds because it has many attractive properties. Dendrimer provides a versatile, compositionally and structurally controlled architecture to construct nanomedicine. This study was aimed at developing a novel electrospun dendrimer-gelatin nanofiber scaffold to best mimic natural extracellular matrix (ECM) to promote tissue formation and serve as a reservoir for controlled drug delivery. Starburst™ polyamidoamine (PAMAM) dendrimer G3.5 was covalently bonded to the gelatin backbone and electrospun into nanofibers. Doxycycline (DC), which is an effective antibiotic that has the ability to inhibit matrix metalloproteinase, was encapsulated into the nanofiber scaffold. The electrospun DC-gelatin scaffold provides a bacterial free environment for cell growth and tissue regeneration. The resulting dendrimer-gelatin nanofiber scaffold achieved a unique structural configuration where covalently bound three-dimensional dendritic nanospheres were evenly distributed along the elongated dimension of the nanofiber, and both dendrimer and gelatin had numerous functional groups suitable for accommodating multiple functional entities and high payload of drugs. The development of this new scaffold with the capability of delivering multiple functional entities was an important step towards the use of bioactive nanofibers to facilitate tissue regeneration and controlled drug release.

Keywords

biomaterialscanning electron microscopy (SEM)biomimetic (chemical reaction)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1094: Symposium DD – From Biological Materials to Biomimetic Material Synthesis , 2008 , 1094-DD09-07
Copyright
Copyright © Materials Research Society 2008

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References

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