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A Multifunctional Microgripper Capable of Simultaneous Single Cell Manipulation and Associated Ion Sensing

Published online by Cambridge University Press:  30 July 2012

Rachael Daunton ,
Andrew J. Gallant ,
Ritu Kataky  and
David Wood
Rachael Daunton
Affiliation:
Department of Chemistry, Durham University, South Road, Durham DH1 3LE UK School of Engineering and Computing Sciences, Durham University, South Road, Durham DH1 3LE UK
Andrew J. Gallant
Affiliation:
School of Engineering and Computing Sciences, Durham University, South Road, Durham DH1 3LE UK
Ritu Kataky
Affiliation:
Department of Chemistry, Durham University, South Road, Durham DH1 3LE UK
David Wood
Affiliation:
School of Engineering and Computing Sciences, Durham University, South Road, Durham DH1 3LE UK
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Abstract

The successful modification of the tips of a cellular microgripper into ion selective electrodes capable of sensing calcium ions at concentrations as low as 8x10-5 M is described. The modification involves applying the process of adding the components of all solid state ion selective electrodes. Specifically, poly(3,4-ethylenedioxythiophene) (PEDOT) is added to a gold electrode protruding from the microgripper tip; this is then coated with a poly(vinyl chloride) PVC based calcium selective membrane. Excellent Nernstian response was observed from our devices, with calibration slopes of 29.5 ± 2.5 mV/dec.

Keywords

sensormicroelectro-mechanical (MEMS)electrodeposition
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1463: Symposium QQ – Mechanobiology of Cells and Materials , 2012 , mrss12-1463-qq03-03
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

1. Bobacka, J., (2006), Conducting polymer based solid state ion selective electrodes, Electroanal., 18, pp. 7–18 10.1002/elan.200503384Google Scholar
2. Durst, R. A., (2012), Ion-selective electrodes – The early years, Electroanal., 24(1), pp. 15–22 10.1002/elan.201100429Google Scholar
3. Buhlmann, P. and Chen, L. D., (2011), Ion-selective electrodes with ionophore-doped sensing membranes, Supramolecular devices, pp. 2539–2579 Google Scholar
4. Daunton, R.. Gallant, A. J. and Wood, D. (2012), “Manipulation of 10-40 micron diameter cells using a thermally actuated microgripper” Proc. Materials Research Society Meeting, San Francisco, USA, (accepted)Google Scholar
5. Daunton, R., Gallant, A. J., Wood, D. and Kataky, R., (2011), A thermally actuated microgripper as an electrochemical sensor with the ability to manipulate single cells, Chem. Commun., 47, pp. 6446–6448 10.1039/c1cc11904dGoogle Scholar
6. Sulaiman, Y. and Kataky, R., (2012), Effect of monomer modifications on the physical properties of plectropolymerised PEDOT films, J. Electrochem. Soc., 159(2), pp. F1–F9 10.1149/2.019202jesGoogle Scholar
7. Wang, S-H., Chou, T-C. and Liu, C-C, (2003), Development of a solid-state thick film calcium ion-selective electrode, Sens. Act. B, 96, pp. 709–716 10.1016/S0925-4005(03)00571-9Google Scholar
8. Paczosa-Bator, B., Peltonen, J., Bobacka, J., Lewenstam, A., (2006), Influence of morphology and topography on potentiometric response of magnesium and calcium sensitive PEDOT films doped with ATP, Anal. Chim. Acta, 555, pp. 118–127 10.1016/j.aca.2005.08.085Google Scholar