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Microfabrication of Asymmetric, Homogeneous Cell-laden Hydrogel Microcapsules

Published online by Cambridge University Press:  31 January 2011

Tram T. Dang ,
Qiaobing Xu ,
Kaitlin M Bratlie ,
Esther S O'Sullivan ,
Xiao Y Chen ,
Robert Langer  and
Daniel G. Anderson
Tram T. Dang
Affiliation:
tramdang@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Qiaobing Xu
Affiliation:
qxu@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Kaitlin M Bratlie
Affiliation:
kbratlie@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Esther S O'Sullivan
Affiliation:
estherosullivan@hotmail.com, Harvard Medical School, slet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts, United States
Xiao Y Chen
Affiliation:
ivychen@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Robert Langer
Affiliation:
rlanger@mit.edu, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
Daniel G. Anderson
Affiliation:
dgander@mit.edu, Massachusetts Institute of Technology, David Koch Institute for Integrative Cancer Research, Cambridge, Massachusetts, United States
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Abstract

Cell encapsulation has been broadly investigated as a technology to provide immunoprotection for transplanted endocrine cells. Here we develop a new fabrication method that allows for rapid, homogenous microencapsulation of insulin-secreting cells with varying microscale geometries and asymmetrically modified surfaces. Micromolding systems were developed using polypropylene mesh, and the mesh material/surface properties associated with efficient encapsulation were identified. Cells encapsulated using these methods maintain desirable viability and preserve their ability to proliferate and secrete insulin in a glucose-responsive manner. This new cell encapsulation approach enables a practical route to an inexpensive and convenient process for the generation of cell-laden microcapsules without requiring any specialized equipment or microfabrication process.

Keywords

biomaterialbiomedicalmicrostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1239: Symposium VV – Micro- and Nanoscale Processing of Biomaterials , 2009 , 1239-VV01-13
Copyright
Copyright © Materials Research Society 2010

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