Hostname: page-component-76fb5796d-x4r87 Total loading time: 0 Render date: 2024-04-26T11:38:06.643Z Has data issue: false hasContentIssue false
  • English
  • Français

Enhancement of Antibody Binding on SiO2 Love Wave Sensor Surface using (3-glycidoxypropyl)trimethoxysilane

Published online by Cambridge University Press:  01 February 2011

Nicolas Moll ,
Duy Haï Dinh ,
Emilie Pascal ,
Corinne Déjous ,
Jean-Paul Pillot ,
Bernard Bennetau ,
Dominique Rebière ,
Daniel Moynet ,
Yan Mas  and
Jacques Pistré
Nicolas Moll
Affiliation:
moll@ixl.fr, Laboratoire IXL, Equipe capteur, 351, cours de la Libération, Talence, N/A, 33405, France, +33(0)5 40 00 65 40, +33(0)5 56 37 15 45
Duy Haï Dinh
Affiliation:
d.dinh@lcoo.u-bordeaux1.fr, Laboratoire de Chimie Organique et Organométallique, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Emilie Pascal
Affiliation:
e.pascal@yahoo.fr, Laboratoire d'Immunologie et Parasitologie, Université Bordeaux 2 Victor Segalen, 146, rue Léo Saignat, Bordeaux, N/A, 33076, France
Corinne Déjous
Affiliation:
dejous@ixl.fr, Laboratoire IXL, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Jean-Paul Pillot
Affiliation:
j-p.pillot@lcoo.u-bordeaux1.fr, Laboratoire de Chimie Organique et Organométallique, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Bernard Bennetau
Affiliation:
b.bennetau@lcoo.u-bordeaux1.fr, Laboratoire de Chimie Organique et Organométallique, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Dominique Rebière
Affiliation:
rebiere@ixl.fr, Laboratoire IXL, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Daniel Moynet
Affiliation:
daniel.moynet@immol.u-bordeaux2.fr, Laboratoire d'Immunologie et Parasitologie, Université Bordeaux 2 Victor Segalen, 146, rue Léo Saignat, Bordeaux, N/A, 33076, France
Yan Mas
Affiliation:
yan.mas@agur.fr, AGUR, 5, rue de la Feuillée, Bayonne, N/A, 64100, France
Jacques Pistré
Affiliation:
pistre@ixl.fr, Laboratoire IXL, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Get access

Abstract

An acoustic Love wave immunosensor dedicated to detect biological species such as bacteria, viruses or toxins is described in this paper. We present and analyze results of antibody grafting on the Love wave device using GPTS as coupling agent between SiO2 sensor surface and the sensitive biomembrane layer composed of antibodies. Goat anti-mouse (GAM) antibodies at concentrations from 2μg/ml to 50 μg/ml were introduced on the sensor brass cell. The saturation of the sensor surface by antibodies appears at a concentration of 20 μg/ml. The detection threshold of this primary antibody is around 2 μg/ml, and the lowest concentration allowing antigen detection is around 5 μg/ml.

Keywords

sensoracousticbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 915: Symposium R – Nanostructured Materials and Hybrid Composites for Gas Sensors and Biomedical Applications , 2006 , 0915-R01-08
Copyright
Copyright © Materials Research Society 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Homola, J., Present and future of surface plasmon resonance biosensors, Anal. Bioanal. Chem. (2003) 377: 528–539 Google Scholar
2 Zimmermann, C., Rebiére, D., Déjous, C., Pistré, J., Chastaing, E., Planade, R., “A love-wave gas sensor coated with functionalized polysiloxane for sensing organophosphorus compounds,” Sens. Actuators B, Vol. 76, pp. 86–94, 2001.Google Scholar
3 Tamarin, O., Déjous, C., Rebiére, D., Pistré, J., Comeau, S., Moynet, D., Bezian, J., “Study of acoustic Love wave devices for real time bacteriophage detection,” Sens. Actuators B, Vol. 91, Issues 1-3, pp. 275–284, 2003.Google Scholar
4 Harding, G.L., Du, J., Dencher, P.R., Barnett, D., Howe, E., “Love wave acoustic immunosensor operating in liquid,” Sens. Actuators A, Volume 61, June 1997, pp 279–286 Google Scholar
5 Moll, N., Déjous, C., Rebiére, D., Pistré, J., Planade, R. “An improved Love wave oscillator for low concentration chemical sensing application” Proc. of DCIS2004, Nov. 2004, pp 276–280 Google Scholar
6 Darder, M., Takada, K., Pariente, F., Lorenzo, F., Abruna, H.D., “Dithiobissuccinimidyl propionate as an anchor for assembling peroxidases at electrodes surfaces and its application in a H2O2 biosensor” Anal Chem. 1999 Dec 15;71(24): 5530–7.Google Scholar
7 Akerstrom, B., Brodin, T., Reis, K., Bjorck, L., “Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies” J. Immunology, Vol 135, Issue 4 2589–2592.Google Scholar
8 Tsukruk, V.V., Luzinov, I., Julthongpiput, D., “Sticky Molecular Surfaces: Epoxysilane Self-Assembled Monolayers,” Langmuir 15, 1999, pp 3029–3032 Google Scholar
9 Kusnezow, W., Jacob, A., Walijew, A., Diehl, F., Hoheisel, J., “Antibody microarrays: An evaluation of production parameters,” Proteomics, 3, 2003, pp 254–264 Google Scholar