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Electric Field-Assisted Positioning of Neurons on Pt Microelectrode Arrays

Published online by Cambridge University Press:  15 February 2011

Shalini Prasad ,
Mo Yang ,
Xuan Zhang ,
Yingchun Ni ,
Vladimir Parpura ,
Cengiz. S. Ozkan  and
Mihrimah Ozkan
Shalini Prasad
Affiliation:
Department of Electrical Engineering
Mo Yang
Affiliation:
Department of Mechanical Engineering
Xuan Zhang
Affiliation:
Department of Mechanical Engineering
Yingchun Ni
Affiliation:
Department of Cell Biology and Neuroscience
Vladimir Parpura
Affiliation:
Department of Cell Biology and Neuroscience
Cengiz. S. Ozkan
Affiliation:
Department of Mechanical Engineering
Mihrimah Ozkan
Affiliation:
Department of Electrical Engineering Department of Chemical and Environmental Engineering University of California Riverside, CA-92521
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Abstract

Characterization of electrical activity of individual neurons is the fundamental step in understanding the functioning of the nervous system. Single cell electrical activity at various stages of cell development is essential to accurately determine in in-vivo conditions the position of a cell based on the procured electrical activity. Understanding memory formation and development translates to changes in the electrical activity of individual neurons. Hence, there is an enormous need to develop novel ways for isolating and positioning individual neurons over single recording sites. To this end, we used a 3x3 multiple microelectrode array system to spatially arrange neurons by applying a gradient AC field. We characterized the electric field distribution inside our test platform by using two dimensiona l finite element modeling (FEM) and determined the location of neurons over the electrode array. Dielectrophoretic AC fields were utilized to separate the neurons from the glial cells and to position the neurons over the electrodes. The neurons were obtained from 0-2-day-old rat (Sprague-Dawley) pups. The technique of using electric fields to achieve single neuron patterning has implications in neural engineering, elucidating a new and simpler method to develop and study neuronal activity as compared to conventional microelectrode array techniques.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 773: Symposium N – Biomicroelectromechanical Systems (BioMEMS) , 2003 , N4.6
Copyright
Copyright © Materials Research Society 2003

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