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Effects of Charged Substrates on Attachment of Primary Bone Marrow Stromal Cells

Published online by Cambridge University Press:  15 February 2011

Q. Qiu ,
M. Sayer ,
J. Wan ,
M. D. Kawaja ,
X. Shen  and
J. E. Davies
Q. Qiu
Affiliation:
Department of Physics
M. Sayer
Affiliation:
Department of Physics
J. Wan
Affiliation:
Department of Biology
M. D. Kawaja
Affiliation:
Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6
X. Shen
Affiliation:
Center for Biomaterials, University of Toronto, Toronto, Ontario, Canada M5S 1A1
J. E. Davies
Affiliation:
Center for Biomaterials, University of Toronto, Toronto, Ontario, Canada M5S 1A1
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Abstract

The effects of charged substrates on the attachment of rat primary bone marrow stromal cells have been investigated using a novel cell culture chamber. Cells were cultured on the surfaces of a conductive and optically transparent indium tin oxide (ITO) coating. When a voltage was applied to the ITO electrodes, positive and negative charges were induced on the ITO electrodes. After 24 hours exposure to 0.8V, more cells attached to the anode than either the cathode or control both in presence or absence of serum in the culture medium. Protein desorption profiles also indicated that the enhanced attachment of cells to the anode was not controlled by the adsorption of serum proteins. The surface texture of ITO was analyzed using atomic force microscopy, the thickness by transmission electron microscopy, and crystallinity using glancing angle x-ray diffraction.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 414: Symposium Z – Thin Films and Surfaces for Bioactivity and Biomedical Applications , 1995 , 141
Copyright
Copyright © Materials Research Society 1996

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